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A kind of detection method of flavonoid compound type and content in rattan tea

A flavonoid compound and detection method technology, which is applied in the field of detection of the type and content of flavonoid compounds in vine tea, can solve the problems of inability to quantitatively detect the content, restrict the development of vine tea industry, and have huge differences in the types and contents of flavonoid active ingredients.

Active Publication Date: 2021-08-20
湖北省农业科学院中药材研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the types and contents of flavonoid active ingredients in vine tea from different production areas vary greatly, and the quality of vine tea is also different.
Traditional detection methods cannot accurately detect the types of flavonoid active ingredients in vine tea, nor can they quantitatively detect the content of flavonoid active ingredients in vine tea, so it is impossible to accurately evaluate the quality of vine tea, which seriously restricts the development of vine tea industry

Method used

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  • A kind of detection method of flavonoid compound type and content in rattan tea
  • A kind of detection method of flavonoid compound type and content in rattan tea
  • A kind of detection method of flavonoid compound type and content in rattan tea

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Embodiment 1

[0064] Collect vine tea samples from Lichuan and Xi’an origins and number them “Lichuan vine tea” and “Xi’an vine tea” respectively, and use ethanol to ultrasonically extract flavonoids: Grind vine tea into powder, pass through a 400-mesh sieve, and weigh 5 grams of the sieve Put the lower matter in a 250mL beaker, soak in 250mL ethanol solution with a mass concentration of 60% for 18h, and extract with 200w ultrasonic wave for 30min; centrifuge the extract at a speed of 8000r / min for 5min, take the supernatant and rotate it under reduced pressure to obtain the extracted Crude flavonoid extract 4.4378g.

[0065] Dissolve 0.1g of crude flavonoid extract with 100g of methanol, take a sample and use HPLC (model: Agilent 1260), AgilentPoroshell 120EC-C18 (4.6×150mm, 4μm) reverse-phase chromatographic column analysis and detection, the injection volume is 5μL, the detector The detection wavelength is 290nm; the gradient elution program is shown in Table 1:

[0066] Table 1 Gradien...

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Abstract

The invention provides a method for detecting the type and content of flavonoids in vine tea, belonging to the technical field of analysis and detection. The detection method provided by the invention comprises the following steps: roughly extracting the flavonoids in the vine tea sample to obtain the flavonoid extract; dissolving the flavonoid extract, and detecting the Analysis, preliminarily speculate the type of flavonoids in the vine tea sample; compare the retention time and UV absorption spectrum of the estimated flavonoids with the standard respectively to determine the type of flavonoids in the vine tea sample; calculate the vine tea according to the standard curve The concentration of flavonoids in the sample. The method provided by the invention can simultaneously detect the types and contents of the flavonoids in the vine tea sample, and the detection results of the method provided by the invention have high precision, and the relative standard deviations are all below 2.56%.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for detecting the types and contents of flavonoids in vine tea. Background technique [0002] Vine tea is processed from the dried leaves and stems of plants of the family Vitaceae or Snake Grape. Vine tea is widely used as a medicinal and edible drink. Studies have found that vine tea has a series of physiological effects such as anti-oxidation, anti-inflammation, liver protection, anti-tumor, anti-tyrosinase, anti-diabetes, and lowering blood lipids. A series of flavonoids with rich content and important physiological functions, such as dihydromyricetin and taxifolin, were isolated and identified in vine tea. [0003] However, the types and contents of flavonoid active ingredients in vine tea from different production areas vary greatly, and the quality of vine tea is also different. Traditional detection methods cannot accurately detect the types of f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/72G01N30/74
CPCG01N30/7266G01N30/74G01N2030/047
Inventor 李宇何美军黄东海艾伦强张宇
Owner 湖北省农业科学院中药材研究所
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