A kind of detection method of flavonoid compound type and content in rattan tea
A flavonoid compound and detection method technology, which is applied in the field of detection of the type and content of flavonoid compounds in vine tea, can solve the problems of inability to quantitatively detect the content, restrict the development of vine tea industry, and have huge differences in the types and contents of flavonoid active ingredients.
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[0064] Collect vine tea samples from Lichuan and Xi’an origins and number them “Lichuan vine tea” and “Xi’an vine tea” respectively, and use ethanol to ultrasonically extract flavonoids: Grind vine tea into powder, pass through a 400-mesh sieve, and weigh 5 grams of the sieve Put the lower matter in a 250mL beaker, soak in 250mL ethanol solution with a mass concentration of 60% for 18h, and extract with 200w ultrasonic wave for 30min; centrifuge the extract at a speed of 8000r / min for 5min, take the supernatant and rotate it under reduced pressure to obtain the extracted Crude flavonoid extract 4.4378g.
[0065] Dissolve 0.1g of crude flavonoid extract with 100g of methanol, take a sample and use HPLC (model: Agilent 1260), AgilentPoroshell 120EC-C18 (4.6×150mm, 4μm) reverse-phase chromatographic column analysis and detection, the injection volume is 5μL, the detector The detection wavelength is 290nm; the gradient elution program is shown in Table 1:
[0066] Table 1 Gradien...
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