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Separation method and application of edible mushroom agarics basidiospores

A technology of edible mushroom umbrella and separation method, which is applied in the direction of microorganism-based methods, fungi, and separation of microorganisms, which can solve the problems of easy pollution, long time-consuming, and complicated operation, and achieve the goal of overcoming ejection basidiospores and miscellaneous bacteria Mixing, efficiency-enhancing, and controllable effects

Inactive Publication Date: 2019-11-12
INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional spore separation method is to rely on its own biological characteristics to eject the basidiospores, and the obtained basidiospores germinate to form pure mycelium. The conventional spore separation method has the situation that the basidiospores are good and bad. It takes 24-24-24 hours to collect the basidiospores after spore ejection. 48h, it takes a long time, and the operation is prone to pollution and other problems
In the prior art, there are many devices used to assist spore separation. The Chinese patent No. CN105969654B discloses a fungal spore washing filter and a fungal spore separation method. The fungal spores are separated by using the washing bottle and the water flow. Separation and collection, but the device and method are likely to cause problems such as spore pollution; the Chinese patent with the patent number CN201770697U discloses a fungal spore separator, including a material pulverizer, a sedimentation separator, an air circulation system and a fan, which can meet the experimental requirements. Room fungal isolation is complex with small batches and multiple varieties, but the operation is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for isolating edible fungus agaric basidiospores, the specific steps are as follows:

[0026] (1) Selection of parents: select half-ripe fruiting bodies as seed mushrooms according to breeding objectives;

[0027] (2) Collection of basidiospores: cut the cap of the fruiting body by aseptic operation, cut the gills 1-2 mm at the joint between the cap and the stipe, place the cut gills in a test tube containing sterile water, and pass through ultrasonic Shake the washer for 15-30 minutes to make basidiospore suspension;

[0028] (3) Obtaining pure mycelia: the basidiospore suspension was diluted by ten-fold dilution method to make 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 For spore suspensions with different gradient concentrations, the prepared spore suspensions were cultured on plates, cultured at 23-25°C for 7-10 days until the spores germinated, and single colonies with good growth were picked to obtain pure mycelia;

[0029] (4) Confirmation of monokaryotic my...

Embodiment 2

[0038] A method for isolating basidiospores of Agaricus blazei and self-breeding of polyspores, the specific steps are as follows;

[0039] (1) In a sterile environment, use a sterile scalpel to cut out 1-2 mm gills at the joint between the cap and the stipe of the mature fruiting bodies of Agaricus blazei, and put them into a test tube of 10 ml sterile water; Place the test tube in an ultrasonic cleaner and vibrate for 20 minutes to prepare Agaricus basidiospore suspension;

[0040] (2) Add 1ml of the Agaricus blazei basidiospore suspension obtained in step (1) into a 200ml liquid medium Erlenmeyer shaker flask, and perform polyspore self-fertilization shaker shaker. The shaker conditions are speed 150 / min, temperature 25 ℃, cultured for 3 days; the obtained Agaricus blazei mixed mycelia liquid was implanted into PDA plate and cultured for 10 days, and the single colony with fast growth rate and thick and white mycelium was selected;

[0041] (3) Check under a microscope (60...

Embodiment 3

[0044] A method for separating basidiospores of Stropharia grandis and self-breeding of polyspores, the specific steps are as follows;

[0045] (1) In a sterile environment, use a sterile scalpel to cut out 1-2mm gills at the joint between the basically mature fruiting body cap and the stipe of Stropharia stropharia, and put them into a test tube of 10 ml sterile water ; Place the test tube in an ultrasonic cleaner and vibrate for 30 minutes to prepare a suspension of Stropharia grandis basidiospores;

[0046] (2) Add 1ml of the Stropharia stropharia basidiospore suspension obtained in step (1) into a 200ml liquid medium conical shaker flask, and shake the bacteria on a polyspore self-fertilization shaker, the shaker condition is 150rpm / min , the temperature was 22°C, and cultured for 1 day; the obtained mixed Stropharia mycelia liquid was implanted into a PDA plate and cultured for 7 days, and a single colony with fast growth and dense and white mycelium was selected;

[004...

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PUM

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Abstract

The invention relates to a separation method and application of edible mushroom agarics basidiospores. The separation method comprises the steps of selecting parents, collecting basidiospores, obtaining pure mycelia, confirming monocaryon hypha and dicaryon hypha and the like. According to the separation method, the basidiospores are collected simply and quickly through a physical mode, so that the process of collecting the basidiospores is shortened from 2 days to 20-30min, the factors, which make the basidiospores difficult to release, such as unsuitable growth and development time and temperature can be overcome, the pollution rate is reduced, and the basidiospores are obtained efficiently and conveniently.

Description

technical field [0001] The invention belongs to the technical field of edible fungus breeding, and in particular relates to a method for separating basidiospores of edible fungi and its application. Background technique [0002] my country is a big country in the cultivation of edible fungi in the world, and it is the country with the most varieties of edible fungi and the highest output in the world. The production of edible fungi is a cultivation system involving many aspects such as strains, compost, growth environment and cultivation techniques. Among the many link factors that meet the requirements of edible fungi production, strains with high quality, high yield and strong stress resistance are a must. The most important factor is that the pure culture of cultivars with excellent genetic and comprehensive traits is the guarantee for the high-quality development of the edible fungus industry. In production practice, bacteria are often used to isolate the required strai...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12R1/645
CPCC12N1/02C12N1/14
Inventor 杨菁肖冬来张迪黄小菁
Owner INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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