Separation method and application of edible mushroom agarics basidiospores

A technology of edible mushroom umbrella and separation method, which is applied in the direction of microorganism-based methods, fungi, and separation of microorganisms, which can solve the problems of easy pollution, long time-consuming, and complicated operation, and achieve the goal of overcoming ejection basidiospores and miscellaneous bacteria Mixing, efficiency-enhancing, and controllable effects

Inactive Publication Date: 2019-11-12
INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional spore separation method is to rely on its own biological characteristics to eject the basidiospores, and the obtained basidiospores germinate to form pure mycelium. The conventional spore separation method has the situation that the basidiospores are good and bad. It takes 24-24-24 hours to collect the basidiospores after spore ejection. 48h, it takes a long time, and the operation is prone to pollution and other problems
In the prior art, there are many devices used to assist spore separation. The Chinese patent No. CN105969654B discloses a fungal spore

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] Example 1

[0025] A method for separating basidiomycetes of edible fungi toadstools, the specific steps are as follows:

[0026] (1) The selection of parents: select the fruiting body of eighth maturity as the seed mushroom according to the breeding goal;

[0027] (2) Collection of basidiomycetes: cut the fruit body caps aseptically, cut 1-2mm gills at the junction of cap and stipe, put the cut gills in a test tube containing sterile water, and ultrasonic Shake the washer for 15-30 minutes to prepare a basidiospore suspension;

[0028] (3) Obtaining pure mycelium: Dilute the basidiospore suspension by ten-fold dilution method to make 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 Spore suspensions with different gradient concentrations were cultured on a plate, cultured at 23~25°C for 7~10 days until the spores germinated, and single colonies that grew well were picked from them to obtain pure mycelium;

[0029] (4) Confirmation of mono- and double-karyotic hyphae: The pure mycelium obt...

Example Embodiment

[0037] Example 2

[0038] A method for separating basidiospores of Agaricus blazei and self-breeding of polyspores, the specific steps are as follows;

[0039] (1) In a sterile environment, use a sterile scalpel to cut out 1-2mm gills at the junction between the cap and the stalk of the mature fruit body of Agaricus blazei, and put it into a test tube of 10 ml sterile water; Place the test tube in an ultrasonic cleaner and oscillate for 20 minutes to prepare a basidiospore suspension of Agaricus blazei;

[0040] (2) Add 1ml of Agaricus blazei Murrill Basidiospore suspension obtained in step (1) into a 200ml liquid medium triangular shaker flask, and perform polyspore self-crossing shaker shaking. The condition of the shaker is 150 / min and temperature 25 Cultivate for 3 days; implant the mixed mycelium liquid of Agaricus blazei Murrill into a PDA plate and culture for 10 days, and select single colonies with fast growth and dense and white hyphae;

[0041] (3) Check under a microscope...

Example Embodiment

[0043] Example 3

[0044] A method for separating basidiomycetes of Stropharia sphaerocarpa and self-breeding of polyspores, the specific steps are as follows;

[0045] (1) In a sterile environment, use a sterile scalpel to cut 1-2mm gills at the junction of the mature fruit body cap and stalk of Stropharia sphaerocarpa, and put them in a test tube of 10 ml sterile water ; Then put the test tube in an ultrasonic cleaner and oscillate for 30 minutes to prepare a basidiospore suspension of Stropharia sphaerocarpa;

[0046] (2) Add 1ml of the basidiospore suspension of Stropharia sphaerocarpa obtained in step (1) into a 200ml liquid medium triangular shaker flask, and perform a multisporous self-shaking shaker. The shaker condition is 150rpm / min. , The temperature is 22℃, culture for 1d; the obtained Stropharia stropharia mixed mycelium liquid is implanted on a PDA plate and cultured for 7d, and a single colony with a fast growth rate and dense and white hyphae is selected;

[0047] (3)...

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Abstract

The invention relates to a separation method and application of edible mushroom agarics basidiospores. The separation method comprises the steps of selecting parents, collecting basidiospores, obtaining pure mycelia, confirming monocaryon hypha and dicaryon hypha and the like. According to the separation method, the basidiospores are collected simply and quickly through a physical mode, so that the process of collecting the basidiospores is shortened from 2 days to 20-30min, the factors, which make the basidiospores difficult to release, such as unsuitable growth and development time and temperature can be overcome, the pollution rate is reduced, and the basidiospores are obtained efficiently and conveniently.

Description

technical field [0001] The invention belongs to the technical field of edible fungus breeding, and in particular relates to a method for separating basidiospores of edible fungi and its application. Background technique [0002] my country is a big country in the cultivation of edible fungi in the world, and it is the country with the most varieties of edible fungi and the highest output in the world. The production of edible fungi is a cultivation system involving many aspects such as strains, compost, growth environment and cultivation techniques. Among the many link factors that meet the requirements of edible fungi production, strains with high quality, high yield and strong stress resistance are a must. The most important factor is that the pure culture of cultivars with excellent genetic and comprehensive traits is the guarantee for the high-quality development of the edible fungus industry. In production practice, bacteria are often used to isolate the required strai...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12R1/645
CPCC12N1/02C12N1/14
Inventor 杨菁肖冬来张迪黄小菁
Owner INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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