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Immature orange fruit endophytic fungi for generating hesperidinase and method for generating hesperidinase through fermentation

A technology of hesperidinase and endophytic fungi, which is applied in the field of hesperidinase produced by endophytic fungi and its fermentation in Citrus aurantii, which can solve the problems that the production technology is not very mature

Active Publication Date: 2019-11-12
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the continuous deepening of research on hesperetin, it has been found that hesperetin has a good preventive and therapeutic effect on vascular disease. Foreign countries use chemical methods to produce hesperetin. Hesperetin is mainly used as a drug raw material, and related drugs have health care and other curative effects, but the production technology is not very mature. Therefore, there is a large space for the production of hesperetin

Method used

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  • Immature orange fruit endophytic fungi for generating hesperidinase and method for generating hesperidinase through fermentation
  • Immature orange fruit endophytic fungi for generating hesperidinase and method for generating hesperidinase through fermentation
  • Immature orange fruit endophytic fungi for generating hesperidinase and method for generating hesperidinase through fermentation

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Experimental program
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Effect test

Embodiment 1

[0019] Isolation and Screening of Endophytic Fungi from Fructus Aurantii

[0020] Select an appropriate amount of fresh citrus aurantium of equal size and no disease, and clean it with distilled water; soak the obtained citrus aurantium with 75% alcohol under aseptic conditions for 120 seconds, rinse with sterile water for 3 times; then soak with 10% sodium hypochlorite for 60 seconds , and then rinsed 6 times with sterile water. Under aseptic conditions, use the sterile water rinsed for the last time to spread on the PDA plate medium. After cultivation, no microorganisms grow out, indicating that the surface is thoroughly disinfected. Then the sterilized citrus aurantium is pulverized with a pulverizer, the powder of citrus aurantium is placed in a petri dish, added sterile water to make it moist, and then placed in an incubator for natural solid-state fermentation to make it Mildew. Then take an appropriate amount of natural moldy Fructus Citrifolia powder, make a bacteria...

Embodiment 2

[0023] Optimization of fermentation conditions for the production of hesperidinase by endophytic fungi from Citrus aurantium

[0024] The effect of initial pH on the fermentative production of hesperidinase by strain ZS002

[0025] After activating the strains stored on the slant of the test tube, they were inoculated into PDA medium, cultivated in a constant temperature incubator at 30°C for 120 h, and after spore formation, made 10 6 cfu / mL of spore suspension.

[0026] Inoculate the spore suspension in a liquid fermentation medium (100mL / 250mL Erlenmeyer flask) with a hesperidin concentration of 5g / L according to 5% inoculum amount, adjust the pH with sodium hydroxide and hydrochloric acid, and place it at a constant temperature of 34°C Shaking at 150 r / min on a shaker for 96 hours. Fermentation liquid (crude enzyme liquid) was obtained by centrifugation in a refrigerated centrifuge. The crude enzyme solution was inoculated into the liquid transformation culture contain...

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Abstract

The invention discloses immature orange fruit endophytic fungi for generating hesperidinase. The bacterial strain is Aspergillus tamarii( Aspergillus tamarii ) ZS002, and is preserved in the China General Microbiological Culture Collection Center(CGMCC) on June 28, 2019, with the preservation No. of CGMCC NO.18105. In the scheme, enzymes generated by microorganisms are used as a catalyst to catalyze transformation of the hesperidin into hesperetin, the reaction efficiency is high, the selectivity is high, few by-products exist, the steps are simple, and the immature orange fruit endophytic fungi is green, environmental-friendly and pollution-free.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to an endophytic fungus strain producing hesperidinase and a method for fermenting and producing hesperidinase. Background technique [0002] Hesperetin has anti-inflammatory, antibacterial, antioxidant, anti-allergic, anti-viral, anti-bacterial, anti-protozoal, anti-fungal and anti-carcinogenic effects and can prevent bone loss. Hesperetin is the hydrolyzate of hesperidin which removes a molecule of rhamnose and glucose. Its medicinal effect is basically similar to that of hesperidin, but its solubility is better. It is obtained by enzymatic hydrolysis of hesperidin with hesperidinase The anticancer activity of hesperetin has been verified in animal experiments. At present, hesperetin is mainly used in food, medicine and cosmetics. In recent years, with the continuous deepening of research on hesperetin, it has been found that hesperetin has a good preventive and therapeutic effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/24C12P17/06C12R1/66
CPCC12N9/2402C12P17/06C12R2001/66C12N1/145
Inventor 李海云张蕴勃王克猛李子院董新红李霞李培骏单杨
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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