A microbial solidification kit and method for in-situ solidification of calcareous sand

An in-situ solidification and microbial technology, applied in the interdisciplinary field of geological engineering and microbiology, can solve the problems of high cost, long construction period, and great environmental hazards, and achieve the effect of increasing pore channels and saving costs

Active Publication Date: 2021-03-12
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional foundation reinforcement method is to use mechanical energy or artificial materials to physically or chemically reinforce the soil. Physical reinforcement methods include measures such as dynamic compaction of the foundation, replacement of soil cushions, and installation of cement mixing piles. The construction period is long, the cost is high, and the site is required. Supports the deployment of large machinery and equipment, in many cases the application is limited
The chemical reinforcement method is to pour chemical grout into the target rock and soil mass, but because most of the grouting materials are strongly alkaline and biologically toxic, the solidified rock and soil mass cannot grow vegetation, which is too harmful to the environment

Method used

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  • A microbial solidification kit and method for in-situ solidification of calcareous sand
  • A microbial solidification kit and method for in-situ solidification of calcareous sand
  • A microbial solidification kit and method for in-situ solidification of calcareous sand

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The cured object in this embodiment is 95.13% of the calcium-containing amount of 20.13%, and the particle-grade symbols are obtained.

[0065] Table 2 Calcium sand grade used in Example 1

[0066]

[0067] A method of microbial in situ curing calcium sand, using microbial inducing mineralization techniques, with a particle size of greater than 0.50 mm, with a particle size of less than 2 mm, less particle content, less than 25% thickness Sand, or particle size of greater than 0.25 mm exceeds 50% of total weight, particle size of greater than 0.50 mm less than total weight of 50%, in the calcium sand body 2, sequentially spray mass fraction of 20% The acetic acid solution, microbial fluid, and a curable liquid consisting of urea and nutrient solution, curing the calcium sand body 2 to form a glue cubicle 1, and the specific structure is figure 1 As shown, the method of in situ curing calcium sand is specifically:

[0068] 1) figure 2 As shown, at 25 ° C, a acetic acid s...

Embodiment 2

[0074] The cured object in this embodiment is a calcium content of 95.13% of China South China Sea calcium sand, and the particle-grade symbols are compiled.

[0075] Table 3 Calcium sand grade used in Example 2

[0076] The particle content of the particle size is more than 0.075 mm exceeds the total weight of 85%, the particle size of more than 0.25 mm is less than the fine sand of 50%, or the particle size of greater than 0.075 mm exceeds 50%, less than all Raising 85% of the sand, uniformly opened the grout hole on the sand body to be treated, the inner diameter of the grouting hole is 0.1m, the hole is 0.2m, and the adjacent grouting hole is 1.0m, and the square mesh is uniform. The method of in situ curing calcium sand is specifically:

[0077] 1) image 3 As shown, a sufficient calcium sand is collected in the target area, poured into a stirring container containing a acetic acid solution having a mass fraction of 20%, soaking 12h, waiting for acetic acid to react back to...

Embodiment 3

[0083] This embodiment is directed to large-scale construction projects with higher ground base carriers and stability requirements, cured targets are 95.13% of China South China Sea calcium sand, particle grade is equipped with medium sand, see Table 2, use spray and note The slurry binding method is formed on the calcium sand body to be treated, the inner diameter of the grout hole is 0.2m, the hole is 1.0m, and the adjacent grout hole is 1.5m, and the square mesh has uniformly.

[0084] 1) figure 2 As shown, at 25 ° C, a acetic acid solution of 25% is sprayed in the target region calcium sand surface, and the spray amount is 2500 kg / m. 2 Keep 12h;

[0085] 2) The liquid medium mixture of the activated Bacrebaclococci is injected into the soil along the grouting tube by the water pump 10 injecting the grouting hole in the grouting tube. The injection is 500 kg / m. 2 Maintain 1.5 h; the microorganism is gradually diffused along the original pore and acetic acid dissolved, and...

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Abstract

The invention discloses a microbial solidification kit and a method for in-situ solidification of calcareous sand, belonging to the interdisciplinary field of geological engineering-microbiology. A microbial solidification kit of the present invention is used for in-situ solidification of calcareous sand, comprising: a first component containing carboxylic acid; a second component containing microorganisms capable of producing urease; a third component containing urea and nutrient solution components. The method for in-situ solidification of calcareous sand by microorganisms includes the step of partially dissolving the calcareous sand by using the above-mentioned first component. The present invention can efficiently obtain calcium source in the in-situ calcareous sandy soil or in the sandy soil in the nearby area, and then react with microorganisms to deposit calcium carbonate to realize the in-situ solidification effect.

Description

Technical field [0001] The invention belongs to the field of geological engineering-microbial cross disciplines, and more particularly to a method of microbial curing kit and in situ curable calcium. Background technique [0002] Microbiological mineralization is widely existent in nature. Some microorganisms can use their own physiological activities or metabolism in vitro to generate a variety of minerals, these crystalline products have a certain impact on geophysical mechanical properties of geotechnical, in recent years The academic community is widely concerned. Since the properties of the square, there is better strength and durability, well-cured sand, so microorganisms induce calcium carbonate deposition (MICP), which has been produced by Microbial Induced CalciumCarbonate Precipitation, and the simultaneous spirit of microbial geotechnical research. MICP methods utilize some specific ferrous enzyme bacteria, such as sporosarcinapasteurii, hydrolyzing urea in an alkaline...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): E02D3/12C12N1/20C12R1/01
CPCC12N1/20E02D3/12
Inventor 唐朝生李昊尹黎阳吕超刘博谢约翰程青张宽施斌
Owner NANJING UNIV
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