Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Geobacillus thermoleovorans G1201 and application thereof

A technology of Geobacillus and bacteria powder, which is applied in the fields of application, bacteria, animal feed, etc., can solve the problems of lack of decomposed strains, long natural fermentation cycle, and odor, so as to improve germination rate, reduce odor, and wide application foreground effect

Active Publication Date: 2019-11-15
QINGDAO MARINE BIOPHARMACEUTICAL RES INST +1
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, in the process of fermenting and producing organic fertilizer, the natural fermentation method has problems such as too long cycle, serious odor and lack of special decomposing strains.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Geobacillus thermoleovorans G1201 and application thereof
  • Geobacillus thermoleovorans G1201 and application thereof
  • Geobacillus thermoleovorans G1201 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1, Identification of Geobacillus oleophilus G1201

[0026] 1. Morphological identification

[0027] Refer to "Bergey's OF Systematic Bacteriology Second Edition "VolumeThree" carried out morphological identification on G1201, and observed its morphology with transmission electron microscope. The strain was yellowish on LB solid medium, translucent, about 3-4 mm in diameter, irregular, with nicks on the edges ( figure 1 ). Under the transmission electron microscope, the cells are rod-shaped, 0.8-1.2 μm wide, 3.0-3.5 μm long, terminal spores, with flagella ( figure 2 ).

[0028] 2. Amplification and sequence analysis of 16S rRNA gene

[0029] 1) The bacterial genome was extracted referring to the operating instructions of the kit; 2) The genome extracted in the above step was used as a template, and the bacterial universal primer 27F (5'-AGAGTTTGATCCTGGCTCAG-3', SEQ ID No: 2) and 1492R (5'-GGTTACCTTGTTACGACTT- 3', SEQ ID No: 3) for PCR amplification. The ...

Embodiment 2

[0030] Embodiment 2, protease characteristics of Geobacillus oleophilus G1201

[0031] Geobacillus oleophilus G1201 was inoculated on a solid medium plate of skim milk powder, cultured at 65°C for 48 hours, and its protease activity was observed by the size of the transparent circle ( image 3 ). Protease activity was measured by the GB / T23527-2009 Folin phenol method.

[0032] Table 1 Results of enzyme production on bacterial strain G1201 plate

[0033]

Embodiment 3

[0034] Example 3, Preparation of Geobacillus oleophilus G1201 liquid fermentation agent

[0035] Take the preserved Geobacillus oleophilus G1201 and activate it in LB liquid medium, culture it on a shaker at 50-70°C and 180-200rpm for 12-18 hours, and inoculate 30L Fermentation tank, cultivated under aeration conditions for 18 hours, used as seed liquid, and then inoculated in a 1000L fermentation tank with an inoculum amount accounting for 1% of the total mass of the medium, cultured for more than 6 hours, and the cell density was greater than or equal to 10 6 CFU / mL.

[0036] Table 2 Bacteria density under different culture conditions

[0037]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a strain of Geobacillus thermoleovorans G1201 and an application thereof, which relate to the field of microorganisms, and a strain preservation number is CGMCC No. 17647. TheGeobacillus thermoleovorans G1201 can be applied to degradation of feathers and feather meal, livestock manure treatment and organic fertilizer production. A fermenting agent of the invention can degrade feather or feather meal, and the content of a sulfhydryl group after fermentation is 2 to 4 times that before fermentation, and a degradation product can be used as a good feed protein material. The fermenting agent is applied to fermenting chicken manure, the odor can be obviously removed, an extract of chicken manure after fermentation is used for seed germination test, the germination rateis twice that of a control group, which can effectively improve the maturity of an organic fertilizer.

Description

technical field [0001] The invention relates to a strain of Geobacillus oleophilus G1201 and its application, in particular to the application of the strain and starter in the fields of degradation of feathers and / or feather meal, preparation of feed protein raw materials, treatment of livestock and poultry manure, and the like. Background technique [0002] Proteases account for about 75% of the types of industrial enzyme preparations, and in recent years sales have accounted for more than 65% of the entire enzyme preparation market. In addition, protease has a wide range of applications and plays an important role in many fields such as food, tanning, feed, washing, textile and medicine. Proteases can be divided into low-temperature proteases, medium-temperature proteases and high-temperature proteases according to their optimum action temperature. The optimum action temperature of high-temperature proteases is 60-80°C or even higher. Since many production processes such ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A23K10/12A23K10/26A23K10/18C05F15/00C05F11/08C12R1/01
CPCA23K10/12A23K10/18A23K10/26C05F3/00C05F11/08C12N1/205C12R2001/01C05F17/00
Inventor 何增国刘国瑞戴宝汤伟卢德鹏唐涛孙晓雯张军
Owner QINGDAO MARINE BIOPHARMACEUTICAL RES INST
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com