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Substance that prevents antigen-antibody reaction inhibition by body fluid

A technology of body fluid and immune response, which is applied in the fields of biomaterial analysis, preparation of test samples, measuring devices, etc. It can solve the problems of failing to provide a wide range of antigen-antibody responses and hindering powerful inhibitors

Pending Publication Date: 2019-11-15
NIPPON HAM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, although the above-mentioned technologies have a certain effect on limited target antigens such as specific pathogens, they have not been able to provide a strong inhibitor against a wide range of antigen-antibody reactions caused by body fluid components such as saliva. A powerful reaction inhibitor for a wide range of protein antigens and various pathogens containing the antigens

Method used

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  • Substance that prevents antigen-antibody reaction inhibition by body fluid
  • Substance that prevents antigen-antibody reaction inhibition by body fluid
  • Substance that prevents antigen-antibody reaction inhibition by body fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0168] (Example 1) Immunological reaction hindered by saliva

[0169] (1-1) Detection hindrance of Campylobacter antigen due to saliva

[0170] In this example, in order to observe the inhibition of the antigen-antibody reaction in the immunochromatography for the detection of Campylobacter, 8×10 6 The amount of cfu / mL was added to Campylobacter jejuni (Campylobacter jejuni; ATCC29428) dead bacteria solution and stirred to make a sample, which was added dropwise to the Campylobacter detection immunochromatography (NH immunochromatography Campylobacter (Japanese ham Co., Ltd.), the detection line and the quality control line were measured by an immunochromatography reader (manufactured by Hamamatsu PHOTONICS, C10066) ( figure 1 ). The detection line is coated with anti-Campylobacter antibody, and the quality control line is coated with anti-mouse IgG antibody, which reacts with Campylobacter bacteria and anti-Campylobacter antibody respectively.

[0171] As a result, althoug...

Embodiment 2

[0196] (Example 2) Screening of Immune Response Hindering Inhibitors

[0197] (2-1) Screening from surfactants

[0198] First, various surfactants that were conventionally known to have an inhibitory effect on immune-reaction-inhibiting substances in body fluids such as saliva were tried as candidates for immune-reaction-suppressing inhibitors.

[0199] As surfactants, negative tests and positive tests were performed using various nonionic surfactants and amphoteric surfactants confirmed in Patent Document 1 to have a solubilizing effect on mucin in saliva.

[0200] As in Example (1-2) etc., negative test and positive test were performed using saliva sample (G) under the same conditions as in Example (1-1) using NH immunochromatographic Campylobacter and Campylobacter dilution.

[0201] As a result, sufficient effects could not be confirmed for almost all surfactants (data not shown). Those with relatively higher effects are shown below (Table 4).

[0202] When using sodium t...

Embodiment 3

[0227] (Example 3) Study on the inhibitory effect of immune reaction inhibition of compounds having sulfonic acid groups

[0228] (3-1) Restoration effect of reactivity hindrance caused by saliva sample

[0229] With regard to various sulfonic acid group-containing low-molecular-weight compounds having a straight-chain alkyl group, which are expected to have a high effect of inhibiting immune response inhibition from the results of Example (2-4), we observed the compounds used in Example 1 (1-1). Is the impediment of reactivity to quality control lines caused by bovine saliva samples recovered in immunochromatographic assays for foot-and-mouth disease antigens.

[0230] As a result, it was the linear dodecylbenzene sulfonate that recovered the reaction block of the quality control line at the lowest concentration. Therefore, it is estimated that linear dodecylbenzenesulfonic acid has the highest inhibitory removal effect (Table 8).

[0231] Furthermore, regarding various alk...

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Abstract

The purpose of the present invention is to: provide an agent that effectively suppresses inhibition of antigen-antibody reaction in an immunoassay using a sample containing a body fluid, in particular, a component derived from a biological mucosal membrane, such as saliva; and to suppress false positive and false negative results in the immunoassay. The present invention provides an immune reaction inhibition suppressing agent that suppresses immune reaction inhibition action derived from a body fluid in an immunoassay sample, the immune reaction inhibition suppressing agent being characterized by including either compound (1) or (2). (1) Sulfonic acid compound represented by the formula R1-SO3H or a salt thereof. (In the formula, R1 is: a straight-chain C5-C30 alkyl group; a straight-chain C1-C3 0 alkyl group substituted by a carbocyclic aromatic group having at least one C5-C30 alkyl group; or a carbocyclic aromatic group having at least one straight-chain C5-C30 alkyl group. These groups may include a substituent group.) (2) Quaternary ammonium ion represented by the formula N+-R2R3R4R5 or a salt thereof. (In the formula, R2-R5 are each independently a straight-chain C1-C30 alkyl group, or a carbocyclic aromatic group substituted by at least one straight-chain C5-C30 alkyl group. These groups may include a substituent group.)

Description

technical field [0001] The present invention relates to providing an inhibition inhibitory substance that inhibits antigen-antibody reaction inhibition caused by body fluid components (especially saliva and other components derived from biological mucosa) in immunoassays, and a rapid, simple and accurate method for using the inhibition inhibitory substance. Immunoassay. Background technique [0002] In the diagnosis of human and animal diseases, various simple examination methods are currently developed. Simple diagnostic methods are mainly diagnostic methods that use specific antibody binding reactions (antigen-antibody reactions) against target substances (pathogens, etc.) to be detected. agglutination) etc. are widely known. Since the results can be obtained quickly and easily after samples are collected from patients and animals, it is possible to start treatment when the symptoms are in the initial state. Therefore, the importance of simple test reagents and kits is i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/566
CPCG01N33/54393G01N33/56922G01N33/56916G01N2800/24G01N2800/26G01N2333/205G01N2333/245G01N2333/09G01N33/56983G01N33/566G01N1/28G01N33/569G01N33/56911
Inventor 竹内江里浦山佳那松本贵之森冈一树山川睦吉田和生菅野彻深井克彦
Owner NIPPON HAM
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