Time-resolved CARS microscopy imaging device and method based on fiber birefringence effect

Abstract

The invention discloses a time-resolved CARS microscopic imaging device and method based on fiber birefringence effect. The device at least includes a femtosecond laser, a power adjustment part, a Stokes optical path part, a pump light pulse and a detection light pulse optical path. part, short-pass dichroic mirror and CARS microscopic imaging system; a part of the ultrashort pulse is incident along the angular bisector of the fast axis and slow axis of the polarization-maintaining fiber, and the difference in refractive index between the fast axis and slow axis of the polarization-maintaining fiber is used to The fast-axis light pulse and the slow-axis light pulse at the output end of the polarization-maintaining fiber are delayed relative to each other, and are used as pump light pulses and detection light pulses respectively. At the same time, the other part of the ultra-short pulse is used to input highly nonlinear polarization-maintaining photons. Crystalline fiber generates wavelength red-shifted optical soliton pulses through the soliton self-frequency shift effect, and the wavelength red-shifted optical soliton pulses are used as Stokes light pulses. The present invention reduces the complexity of the traditional time-resolved CARS microscopy imaging system while realizing the measurement of time-resolved CARS signals.

Description

technical field [0001] The invention belongs to the field of nonlinear optical fiber and spectral resonance microscopic imaging, in particular to a time-resolved CARS microscopic imaging device and method. Background technique [0002] CARS microscopic imaging technology is a label-free, non-contact and chemically selective microscopic imaging technology that uses the resonance energy level information of molecules in substances to perform resonance spectrum imaging. Its essence is pump light, probe light, and the resonance energy level of the sample to be measured The four-wave mixing effect, when the frequency difference between the pump light and the Stokes light is equal to the resonance energy level of the target chemical bond in the sample to be tested, and the three meet the phase matching conditions, the anti-Stokes of the sample to be tested will be excited X Raman scattering signal, that is, CARS signal. However, in the traditional CARS microscopic imaging system,...

AI Technical Summary

Problems solved by technology

In the commonly used time-resolved CARS microscopic imaging system, in order to generate the time delay between the pump light pulse and the probe light pulse, the method of adding a pump laser or adding a spatial optical delay line is usually used, which undoubtedly increases the space optical path of the system. The complexity and the cost of operation and maintenance are not conducive to the use and development of time-resolved CARS microscopy imaging system

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