A control method for delaying the age of aphid
A technology of age and age of aphids, applied in the field of regulation and control of delaying the age of aphids, to achieve the effects of increasing parasitism rate, improving consistency and low cost
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Embodiment 1
[0030] Example 1 Effects of Juvenile Hormone Analogues on Age of Aphid
[0031] Test method: five reagents, ZR-777, ZR-512, ZR-515, juvenile hormone Ⅲ and fenoxycarb, were used to detect the effects of different juvenile hormone analogues on the age of aphids. Treatment group: Dilute the mother solutions of the five reagents with distilled water to 5 concentrations of 5000, 1000, 200, 40, and 8ng / μl; Control group: Mix distilled water and acetone at a volume ratio of 9:1 to make a control solution .
[0032] Immerse in each prepared control solution or treatment solution, 2nd instar larvae without wings of the same size, soak for 5-6 seconds, take out, and blot dry with filter paper, set up 3 repetitions; , Place 6 tobacco leaf discs with a diameter of 2.5 cm at intervals, then pick up 6 treated larvae, and repeat 3 times for each treatment. After treatment, under the conditions of temperature 25±1℃, relative humidity 70±5%, and photoperiod 14L:10D, observe and record the ef...
Embodiment 2
[0062] Example 2 Effects of Juvenile Hormone Analogues on Litter Size of Aphis persicae
[0063] Test method: in embodiment 1, when the 5th instar female aphid grown from the treated Aphis persicae F0 produces the offspring F1, the 1st instar larvae produced by it are connected to the petri dishes containing tobacco leaves respectively, and the control And the treatment settings are the same as "using larvae liquid immersion method", 18 animals in each treatment or control, repeated 3 times, 324 animals in 6 treatments (including controls). And after inoculation treatment, observe and record the growth of aphid (molting, calving).
[0064] test results:
[0065] Juvenile hormone analogues against aphids contemporary (F 0 ) effects on litter size, such as figure 1 shown. From figure 1 It can be seen that different reagents have significant effects on the average litter size of Aphis persicae. The average total litter size per aphid in the control group was 28.45. Compar...
Embodiment 3
[0067] Example 3 Effects of Juvenile Hormone Analogs on the Growth and Development of Potted Plant Aphids
[0068]Test method: According to the reagent with good effect of delaying the age of Aphis persicae screened and obtained in Example 1, the spray treatment test of A. persicae on potted tobacco plants was carried out.
[0069] Treatment group: prepare ZR-515 with a concentration of 5000 ng / μl, ZR-515 with a concentration of 1000 ng / μl and ZR-777 with a concentration of 200 ng / μl;
[0070] Control group: Control group: Distilled water and acetone were mixed at a volume ratio of 9:1 to make a control solution.
[0071] The control and treatment solutions were evenly sprayed with a hand-held spray bottle on the leaf cores of tobacco plants connected with 30 2nd instar nymphs of the same size until no water dripped. For each treatment or control, 3 tobacco plants were repeated 3 times, a total of 12 plants. Observe and record the growth of Aphis persicae (molting and calvin...
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