Method for detecting fat-soluble vitamins in blood sample

Abstract

The invention discloses a method for detecting fat-soluble vitamins in a blood sample and belongs to the technical field of vitamin detection. The method includes the pretreatment of the blood samplewith a simple liquid-liquid extraction method, and the quantitative analysis with an internal standard curve method with the relative retention time of five fat-soluble vitamins and detected quantitative ion-pair information by using the methods of chromatographic separation and mass spectrometry detection. The technical indicators such as a recovery rate, a detection limit and precision of the method of the invention meet the requirements, the operation is simple, the error is small, the analysis time is short, the reproducibility is good, the requirements of clinical sample detection are met, and the method is easy to promote and popularize.

Description

technical field [0001] The invention belongs to the technical field of vitamin detection, in particular to a method for detecting fat-soluble vitamins in blood samples. Background technique [0002] Vitamins participate in the normal metabolism of the human body and are one of the essential nutrients to maintain normal life activities. They are divided into two categories: fat-soluble vitamins and water-soluble vitamins. Fat-soluble vitamins include vitamins A, D, E, and K, and water-soluble vitamins include B vitamins. and vitamin C. Vitamins participate in the metabolism of substances and energy in the body, and are indispensable nutrients for normal metabolic regulation and maintenance of physiological functions. When the body lacks vitamins, it will lead to metabolic disorders and even serious diseases. [0003] Among the fat-soluble vitamins, vitamin A plays a very important role in maintaining normal vision and light sensitivity, hematopoietic system function, promoti...

AI Technical Summary

Problems solved by technology

But there are mainly the following problems: first, vitamins in the blood such as 25-hydroxyvitamin D are mainly combined with the serum binding protein DBP in the human body, and there are a large number of high-affinity binding proteins in the human body, so there is serious matrix interference in the detection; second , complex pretreatment process, long analysis time, low throughput, and poor method specificity

However, the current ultra-high performance liquid chromatography tandem mass spectrometry can only quantitatively analyze one or two vitamins at the same time, and the detection time is long, which increases the complexity of the operation, prolongs the detection time and increases the detection time when multiple vitamins are determined. Cost, not conducive to clinical promotion and popularization

[0005] For example, the authorized announcement number CN105158394B, which is called a method for simultaneously detecting multiple fat-soluble vitamins in blood samples, discloses a method capable of detecting six fat-soluble vitamins in human blood, which is also suitable for detection by chromatography and mass spectrometry, but it is not The use of isotopic internal standards will cause some sample loss during sample pretreatment, especially during n-hexane extraction; during mass spectrometry ionization, isotopic internal standards can also offset the matrix effect of the background, so failure to use isotopic internal standards will cause detection results It has certain uncontrollable risks; secondly, the content of some vitamins such as 25-hydroxyvitamin D2 in human blood samples is low, and the lower limit of quantification needs to be lowered to meet the needs of different clinical samples; in addition, according to the gradient elution conditions provided by it It shows that it takes 4.8mim, which takes a long time

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