Method for performing ovulation at same period and performing semen deposition in timing manner
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A technology of timed insemination and estrus cycle, applied in the field of animal husbandry, can solve the problems of long production cycle and low conception rate in cattle breeding
Inactive Publication Date: 2019-11-26
JILIN AGRICULTURAL UNIV
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[0005] The purpose of the present invention is to provide a method of synchronous ovulation and timing insemination with a high conception rate to solve the problems of long production cycle in the cattle breeding industry and low conception rate of existing synchronous ovulation-timed insemination
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Embodiment 1
[0027] Example 1 A method of synchronous ovulation timing insemination
[0028] A method for timing insemination during synchronous ovulation, which includes:
[0029] 1) Take healthy young Simmental cattle with moderate body condition (body condition score between 2.5-4.0), and check the basic condition of the ovaries by B-ultrasound at the beginning, and divide them into luteal phase (luteal body diameter > 0.3 cm), follicular phase ( Follicle diameter > 0.3 cm), resting phase (no corpus luteum and follicles), normal reproductive organs, no reproductive diseases;
[0030] 2) Determine any day of the estrus cycle as 0d, and inject melatonin subcutaneously on the 4th day (-4d) counting from 0d, and the injection dose is 12mg / 50kg cattle body weight;
[0031] 3) Inject GnRH (100µg) intramuscularly into the cattle on the 0th day, inject PG (0.25mg) intramuscularly into the cattle on the 7th day (+7d) counting from 0d, and inject GnRH (100µg) intramuscularly and subcutaneously i...
Embodiment 2
[0032] Example 2 Beef cattle synchronous ovulation timing insemination experiment
[0033] 1. Experimental animals
[0034] A total of 86 young Simmental cattle aged over 18 months who were healthy and moderate in body condition (body condition score between 2.5 and 4.0) were selected. At the beginning of the experiment, the basic condition of the ovary was checked by B-ultrasound in all experimental cattle, and they were divided into luteal phase (luteal body diameter > 0.3 cm), follicular phase (follicle diameter > 0.3 cm), quiescent phase (without corpus luteum and follicles), normal reproductive organs, and no reproductive diseases.
[0035] 2. Grouping
[0036] The selected 86 test cows were randomly divided into 4 groups: control group (CTR, n=25), melatonin group (MT, n=20), melatonin+HCG group (MT+HCG, n=21) , twice melatonin group (2MT, n=20) ( figure 1 );
[0037] 1) Control group: GnRH (100 µg) was injected intramuscularly for the first time on day 0, PG (0.25 m...
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Abstract
The invention discloses a method for performing ovulation at the same period and performing semen deposition in a timing manner. The method comprises the steps of (1) injecting melatonin of which thedosage is determined according to the standard of 10-20mg / 50kg based on the weight of cattle under the cattle skin at the time of 4d before any day of the estrus of the cattle; (2) performing intramuscular injection of 80-200 [mu]g of GnRH to the cattle at the time of 0d, and performing intramuscular injection of 0.1-0.5mg of PG to the cattle at the time of 7d; and (3) after 50-60h, performing intramuscular injection of 80-200 [mu]g of GnRH to the cattle, performing subcutaneous injection of melatonin of which the dosage is determined according to the standard of 10-20mg / 50kg based on the weight of the cattle, and performing artificial insemination. According to the method disclosed by the invention, after insemination, the pregnancy rate reaches 75%, through determination, the MDA concentration in blood of cows is reduced, and the SOD concentration, the GSH-PX concentration and the CAT concentration are increased. The method disclosed by the invention has the advantages that oestrus identification before semen deposition can be omitted when the method is used; and fertilization and pregnancy are at the same period, management and breeding at the lactation period can be unified, and scientific raising management of calves and breeding cattle in a large scale is well done through concentrating energy.
Description
technical field [0001] The invention belongs to the technical field of animal husbandry, and in particular relates to a method for synchronous ovulation and timing insemination. Background technique [0002] my country's beef cattle breeding model is mainly based on stocking and house feeding. The stocking mode is mainly in mountainous areas. Due to factors such as early spring, the breeding rate of postpartum estrus is low, and the cows are raised on the mountain. After the cows are in estrus, they mate naturally, which seriously limits the promotion and reproduction of artificial insemination technology for beef cattle. Increased efficiency. House feeding is the main breeding mode in beef cattle farming. Long-term captivity of cows leads to malnutrition, lack of trace elements, insufficient exercise, etc., which affects the reproductive ability of cows. It mainly causes cows with no obvious estrus symptoms or only ovulation without estrus, and persistent corpus luteum i...
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