Preparation method of amperometric aptasensor for detecting pesticide residues
An aptamer sensor and pesticide residue technology, applied in the field of electrochemical biosensors, can solve problems such as cell, nerve axon influence, behavior change, etc., and achieve the effects of low price, fast response, and simple preparation method
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[0027] The present application provides a preparation method of an amperometric aptamer sensor for detecting pesticide residues, comprising the following steps in sequence:
[0028] 1) Polishing, cleaning, activating and testing the glassy carbon electrode, and obtaining a pretreated glassy carbon electrode after completion;
[0029] 2) The ordered mesoporous carbon is added to the chitosan solution and mixed to obtain the ordered mesoporous carbon-chitosan suspension, and then the ordered mesoporous carbon-chitosan suspension is drop-coated on the step 1) to prepare. The surface of the obtained pretreated glassy carbon electrode is then dried, and the ordered mesoporous carbon-chitosan suspension becomes an ordered mesoporous carbon-chitosan layer after drying;
[0030] 3) adding nano titanium dioxide to the chitosan solution and mixing to obtain a titanium dioxide-chitosan suspension, and then drop-coating the titanium dioxide-chitosan suspension on the ordered mesoporous ca...
Embodiment 1
[0046] Example 1: Cyclic voltammetry characterization of the preparation process of amperometric aptamer sensors
[0047] The CV electrochemical characterization of the bare glassy carbon electrode and the glassy carbon electrode modified in each step is carried out, and the curves are as follows figure 2 As shown, curve a in the figure is the CV curve measured by the pretreated glassy carbon electrode. At this time, the glassy carbon electrode has not been treated with any treatment, the conductivity is poor, and the measured current is small;
[0048] When the ordered mesoporous carbon-chitosan suspension droplets were coated on the surface of the glassy carbon electrode ( figure 2 Middle curve b), the current increases significantly due to the good electrical conductivity of mesoporous carbon;
[0049] When the titanium dioxide-chitosan suspension was drop-coated on the above glassy carbon electrode ( figure 2 In the middle curve c), the current increases significantly...
Embodiment 2
[0053] Example 2: Working curve of amperometric aptamer sensor for detecting chlorpyrifos
[0054] like image 3 As shown in A, under the optimized test conditions, the electrodes were incubated in standard solutions of chlorpyrifos with different concentrations for 40 min and then tested by DPV.
[0055] image 3 In B, it can be seen that the difference between the current peaks and the concentration of chlorpyrifos has a good linear relationship, and a good linear relationship is obtained in the range of 0ng / mL to 90ng / mL. The linear regression equation is Y=4.637X+1.7, its The linear correlation number was 0.994, and the detection limit was 0.04 ng / mL (S / N=3).
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