Piceatannol oligomer, preparation method therefor and application of piceatannol oligomer

A piceatannol and oligomer technology, which is applied in the field of separation and purification and extraction of plant components, can solve the problems of difficult piceatannol oligomers and high polarity, and achieves high sensitivity, fast separation speed and high The effect of separation

Active Publication Date: 2019-12-03
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the fact that picatanol components have multiple hydroxyl groups and high polarity, it is difficult to quickly obtain high-purity picatanol oligomers by existing separation methods

Method used

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  • Piceatannol oligomer, preparation method therefor and application of piceatannol oligomer
  • Piceatannol oligomer, preparation method therefor and application of piceatannol oligomer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The preparation method of the piceatanol oligomer of the present embodiment, comprises the steps:

[0069] Step 1: High Speed ​​Counter-Current Chromatography Separation

[0070] The separated two-phase solvent system is obtained by mixing n-hexane, ethyl acetate, methanol and water in a volume ratio of 10:20:10:28, and then standing to separate layers.

[0071] The upper phase of the two-phase solvent system is used as the stationary phase, and the lower phase is used as the mobile phase.

[0072] First inject the stationary phase into a high-speed countercurrent chromatography column (300mL) at a temperature of 25°C at a flow rate of 30mL / min. After the stationary phase is filled with the high-speed countercurrent mL / min into the high-speed countercurrent chromatography column.

[0073] After the dynamic equilibrium of the whole system is established, 72.5 mL of the stationary phase flows out at this time, and the retention rate of the stationary phase is S f The c...

Embodiment 2

[0121] The preparation method of the piceatanol oligomer of the present embodiment, comprises the steps:

[0122] Step 1: High Speed ​​Counter-Current Chromatography Separation

[0123] The separated two-phase solvent system is obtained by mixing n-hexane, ethyl acetate, methanol and water in a volume ratio of 10:18:10:24, and then standing to separate.

[0124] The upper phase of the two-phase solvent system is used as the stationary phase, and the lower phase is used as the mobile phase.

[0125] First inject the stationary phase into a high-speed countercurrent chromatography column (300mL) at a temperature of 25°C at a flow rate of 30mL / min. After the stationary phase is filled with the high-speed countercurrent chromatography column, adjust the rotation speed to 800r / min, and then inject the mobile phase at a flow rate of 2.0 mL / min into the high-speed countercurrent chromatography column.

[0126] After the dynamic equilibrium of the whole system is established, 77.4 mL ...

Embodiment 3

[0136] The preparation method of the piceatanol oligomer of the present embodiment, comprises the steps:

[0137] Step 1: High Speed ​​Counter-Current Chromatography Separation

[0138] The separated two-phase solvent system is obtained by mixing n-hexane, ethyl acetate, methanol and water in a volume ratio of 10:22:10:32, and then standing to separate.

[0139] The upper phase of the two-phase solvent system is used as the stationary phase, and the lower phase is used as the mobile phase.

[0140] First inject the stationary phase into a high-speed countercurrent chromatography column (300mL) at a temperature of 25°C at a flow rate of 30mL / min. After the stationary phase is filled with the high-speed countercurrent chromatography column, adjust the rotation speed to 900r / min, and then inject the mobile phase at a flow rate of 2.0 mL / min into the high-speed countercurrent chromatography column.

[0141] After the dynamic equilibrium of the whole system is established, 76.5 m...

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Abstract

The invention discloses a piceatannol oligomer, a preparation method therefor and an application of the piceatannol oligomer and belongs to the technical field of extraction, separation and purification of plant ingredients. A specific structural formula of the piceatannol oligomer is any one selected from structures as follows: formulae shown in the description. The invention further discloses the preparation method for the piceatannol oligomer and the application of the piceatannol oligomer. The piceatannol oligomer disclosed by the invention is derived from natural plants and is of a brand-new structure; a pair of new structural isomers can be formed through forming a five-membered ring by 7,8-site double bonds of cis-piceatannol and a phenolic hydroxyl group of epicatechin and are monopolymer derivatives of piceatannol; or a pair of new isomers with high-symmetrical structures are formed through forming a five-membered ring by 7,8-site double bonds of cis-(trans-)piceatannol and aphenolic hydroxyl group and are trimers of the piceatannol.

Description

technical field [0001] The invention relates to a piceatanol oligomer, a preparation method and application thereof, and belongs to the technical field of extraction, separation and purification of plant components. Background technique [0002] Picetannol (3,3',4,5'-tetrahydroxystilbene, English name piceatannol), resveratrol 3-hydroxylation analogue, is a stilbene compound with multiple hydroxyl substitutions , mainly found in passion fruit, grapes, sugar cane and other plants. Pharmacological studies have shown that picatanol can effectively scavenge free radicals, exert anti-inflammatory, anti-cancer and cardiovascular protection biological activities, and has high development and utilization value. Because the structure of picatanol contains multiple hydroxyl groups, it is easy to undergo polymerization to form a series of compounds with variable chemical structures, that is, oligomers of picatanol (including monomers and dimers of picatanol) , trimer, tetramer or the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61K8/49A61Q19/02A61P3/10A61K31/352A61K31/343A23L33/105
CPCA23L33/105A23V2002/00A61K8/4973A61K8/498A61P3/10A61Q19/02C07B2200/07C07D493/04A23V2200/328A23V2250/2132
Inventor 宁德生潘争红符毓夏李连春
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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