Preparation method for extracting flavonoid glycoside from pteris cretica var. laeta

A technology of Pteris fern and flavonoid glycosides, which is applied in the field of preparation of flavonoid glycosides extracted from Pteris vulgaris rough, can solve the problems of high production cost, low yield, cumbersome purification process, etc., and achieve great reference value, high purity, and experimental The effect of accurate data

Active Publication Date: 2019-12-03
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on Pteris genus, and the existing flavonoid glycoside extraction methods or purification processes are relatively cumbersome, time-consuming, high production costs, or low yields, and there is no large-scale production

Method used

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  • Preparation method for extracting flavonoid glycoside from pteris cretica var. laeta
  • Preparation method for extracting flavonoid glycoside from pteris cretica var. laeta
  • Preparation method for extracting flavonoid glycoside from pteris cretica var. laeta

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Effect test

Embodiment 1

[0024] A preparation method for extracting flavonoid glycosides from Pteris vulgaris; the specific steps are:

[0025] (1) extracting and extracting the rough Pteris fern stem and leaf with an organic solvent;

[0026] Dry and rough 25kg of Pteris fern stems and leaves, pulverize, place the pulverized raw material particles in a 200L extraction tank, soak for 6 hours with 95% ethanol with a material-to-liquid ratio of 1:5 (kg / L), and then heat and reflux for extraction 3 times, each time for 1 hour, the extract was recovered under reduced pressure, and concentrated to obtain 1.2 kg of the crude extract of the fern fern stem and leaf extract. The extract was extracted with petroleum ether, ethyl acetate and n-butanol respectively to obtain four parts of petroleum ether (260g), ethyl acetate (400g), n-butanol (300g) and water (350g).

[0027] (2) Polyamide resin column chromatographic separation and medium pressure preparation;

[0028] Wherein the ethyl acetate part is eluted...

Embodiment 2

[0034] A preparation method for extracting flavonoid glycosides from Pteris vulgaris; the specific steps are:

[0035] (1) extracting and extracting the rough Pteris fern stem and leaf with an organic solvent;

[0036] Dry and rough 25kg of Pteris fern stems and leaves, pulverize, place the pulverized raw material particles in a 200L extraction tank, soak for 8 hours with 95% ethanol with a material-to-liquid ratio of 1:5 (kg / L), then heat and reflux for extraction 3 times, each time for 1 hour, the extract was recovered under reduced pressure, and concentrated to obtain the crude extract of the stem and leaf of Pteris vulgaris; 1.2kg. The extract was extracted with petroleum ether, ethyl acetate and n-butanol respectively to obtain four parts of petroleum ether (260g), ethyl acetate (400g), n-butanol (300g) and water (350g).

[0037] (2) Polyamide resin column chromatographic separation and medium pressure preparation;

[0038] Wherein the ethyl acetate part is eluted by po...

Embodiment 3

[0042] A preparation method for extracting flavonoid glycosides from Pteris vulgaris; the specific steps are:

[0043] (1) extracting and extracting the rough Pteris fern stem and leaf with an organic solvent;

[0044]Dry and rough 25kg of Pteris fern stems and leaves, pulverize, place the pulverized raw material particles in a 200L extraction tank, soak for 8 hours with 95% ethanol with a material-to-liquid ratio of 1:5 (kg / L), then heat and reflux for extraction 4 times, 1 hour each time, the extract was recovered under reduced pressure, and concentrated to obtain the crude extract of the stem and leaf of Pteris vulgaris; 1.2kg. The extract was extracted with petroleum ether, ethyl acetate and n-butanol respectively to obtain four parts of petroleum ether (260g), ethyl acetate (400g), n-butanol (300g) and water (350g).

[0045] (2) Polyamide resin column chromatographic separation and medium pressure preparation;

[0046] Wherein the ethyl acetate part is eluted by polyami...

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Abstract

The invention belongs to the application field of compound extraction, and discloses a preparation method for extracting flavonoid glycoside from pteris cretica var. laeta. The preparation method comprises the specific steps of (1) collecting and extracting pteris cretica var. laeta stems and leaves with an organic solvent; (2) performing polyamide resin column chromatography separation and middle-pressure liquid chromatography preparation; and (3) performing high efficiency liquid phase chromatographic detection chromatographic detection preparation. The prepared flavonoid glycoside is high in purity, and through high efficiency liquid phase chromatography, the content of the flavonoid glycoside can achieve 95% or above through detection. The extraction and preparation method is simple tooperate, and the prepared flavonoid glycoside is high in purity, low in production cost and convenient for industrial production.

Description

technical field [0001] The invention belongs to the application field of compound extraction, and specifically relates to a preparation method for extracting flavonoid glycosides from Pteris crassa. Background technique [0002] Flavonoids are a class of polyphenolic antioxidants, and they are the main active ingredients of commonly used clinical traditional Chinese medicinal materials such as kudzu root, psoraleae, scutellaria radix, ginkgo biloba, seabuckthorn, and sophora japonica. Such compounds are numerous and complex in structure, and have many important physiological activities, such as anti-tumor, anti-diabetes, liver protection, prevention and treatment of cardiovascular and cerebrovascular diseases, anti-oxidation, anti-bacterial, anti-inflammation, etc. Modern pharmacological studies have shown that flavonoids, as a class of important natural compounds, have significant curative effects on chemical liver injury, immune liver injury, drug-induced liver injury and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H17/07C07H1/08Y02A50/30
Inventor 李杨吴娇尹瀚林石玉生李春斌
Owner DALIAN NATIONALITIES UNIVERSITY
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