Cell membrane surface modification method mediated by transglutaminase

Abstract

The invention discloses a cell membrane surface modification method mediated by glutamine transaminase. The cell membrane surface modification method mediated by transglutaminase provided by the present invention comprises the following steps: using transglutaminase to connect the modification to the substrate protein on the cell membrane surface of the target cell; the modification contains glutamine Amide residues; the substrate protein contains lysine residues. The present invention has developed a precise and controllable cell membrane surface modification method based on natural enzymatic reactions. The platform is guided by the improvement of cell function, without changing the physiological characteristics of the cell itself, and based on cell basic research and cell therapy. With the diversity and variability of functional fragments as the medium, it is characterized by simple operation, fast response and high repeatability, and provides a new idea for regenerative medicine such as drug development, cell therapy and basic research.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a cell membrane surface modification method mediated by glutamine transaminase. Background technique [0002] Currently reported cell modification methods include: 1. Conditional stimulation; 2. Gene modification; 3. Cell membrane modification. [0003] Conditional stimulation mainly refers to the stimulation of cells through physical or chemical factors, so that cells respond to specific stimuli. Such as hypoxic culture, drug intervention, growth factor induction, etc., are beneficial for cells to up-regulate the expression of certain target molecules to achieve the purpose of changing cell characteristics. But such stimuli can lead to a series of uncontrollable changes in cells in terms of overall genes and epigenetics, so there are safety risks. For example, pre-stimulating cell differentiation will greatly improve its immunogenicity. If it is used for cell therapy, it ...

AI Technical Summary

Problems solved by technology

The most conventional method of cell membrane surface modification is mainly chemical method, but the process of chemical modification is very complicated, requiring multi-step operations to connect polypeptides to the cell membrane, which will affect the production of cell activity to varying degrees, and the most obvious The disadvantage is the randomness of the connection substrate

At present, the physical method is mainly based on the principle of similarity and compatibility between the cell membrane and the modifier, so that the modifier is embedded on the cell membrane. In this method, the treatment requirements of the modified object are relatively high, and after modification, it exists on the cell membrane for a long time, and the half-life is relatively short. long

However, the existing biological modification methods are mainly aimed at the specific enzyme molecules of a specific cell, which is not universal.

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