Method for separating and purifying zeaxanthin from marigold oleoresin
A technology for the separation and purification of zeaxanthin, which is applied in the direction of organic chemistry, can solve the problems of raw material waste cost, increase, and unsatisfactory yield, and achieve the effect of improving purity, high purity, and speeding up the resolution rate
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Embodiment 1
[0034]1) Mix marigold oleoresin with ethyl acetate-ethanol solution (ethyl acetate:ethanol V / V, 6.5:1) at a mass ratio of 1:7, stir and dissolve; then transfer to a 6 kg macropore In the analysis column of the adsorption resin, the adsorption flow rate is 2BV / h, and the temperature is 50°C; after the adsorption is completed, rinse with deionized water until it is colorless; use ethyl acetate-ethanol analysis solution (ethyl acetate:ethanol V / V, 6.5:1 ) for analysis (marigold oleoresin: ethyl acetate-ethanol analysis solution w / w, 1:5), the analysis flow rate is 1BV / h, and the zeaxanthin analysis solution is collected;
[0035] 2) Concentrate the zeaxanthin ester analysis solution to obtain zeaxanthin ester extract; the residual volatiles in the zeaxanthin ester extract are controlled at 8% w / w;
[0036] 3) Hydrolyze zeaxanthin extract, propylene glycol, and 45% w / w potassium hydroxide aqueous solution (w / w, 1:0.5:0.7) in a reactor filled with carbon dioxide, and the temperatur...
Embodiment 2
[0040] 1) Mix marigold oleoresin with ethyl acetate-ethanol solution (ethyl acetate:ethanol V / V, 6:1) at a mass ratio of 1:8, stir and dissolve; then transfer to a 6 kg macropore In the analytical column of the adsorption resin, the adsorption flow rate is 1.5BV / h, and the temperature is 50°C; after the adsorption is completed, rinse with deionized water until it is colorless; use ethyl acetate-ethanol analysis solution (ethyl acetate: ethanol V / V, 6: 1) Perform analysis (marigold oleoresin: ethyl acetate-ethanol analysis solution w / w, 1:6), analysis flow rate 0.8BV / h, and collect zeaxanthin analysis solution;
[0041] 2) Concentrate the zeaxanthin ester analysis solution to obtain zeaxanthin ester extract; the residual volatile matter in the zeaxanthin ester extract is controlled at 6% w / w;
[0042] 3) The zeaxanthin extract, propylene glycol, and 40% w / w sodium hydroxide aqueous solution (w / w, 1:0.7:0.4) were hydrolyzed in a reactor filled with carbon dioxide, and the temper...
Embodiment 3
[0046] 1) Mix and dissolve marigold oleoresin and ethyl acetate-ethanol solution (ethyl acetate:ethanol V / V, 7:1) at a mass ratio of 1:6; In the analysis column of the resin, the adsorption flow rate is 2.5BV / h, and the temperature is 53°C; after the adsorption is completed, rinse with deionized water until it is colorless; use ethyl acetate-ethanol analysis solution (ethyl acetate:ethanol V / V, 7:1 ) for analysis (marigold oleoresin: ethyl acetate-ethanol analysis solution w / w, 1:4), the analysis flow rate is 1BV / h, and the zeaxanthin analysis solution is collected;
[0047] 2) Concentrate the zeaxanthin ester analysis solution to obtain zeaxanthin ester extract; the residual volatiles in the zeaxanthin ester extract are controlled at 5% w / w;
[0048] 3) Hydrolyze zeaxanthin extract, butanol, and 50% w / w potassium hydroxide aqueous solution (w / w, 1:0.3:0.8) in a reactor filled with carbon dioxide, and the temperature of the hydrolysis reaction is 80°C, the hydrolysis reaction...
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