EPA/DHA type lysophospholipid composition and preparation method thereof

A technology of lysophospholipid and phospholipid mixture is applied in the field of biocatalytic preparation of lipids, which can solve the problems of reducing the GPC content of phosphoglycerol choline in modified phospholipids, and achieve the advantages of reducing GPC content, reducing production cost and improving repeatability. Effect

Active Publication Date: 2019-12-13
大渔华创(广州)海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Partial glyceride lipase can be divided into two categories: monoacylglycerol lipase (Monoacylglycerol lipase) and monoglyceride-diglyceride lipase (Mono-and diacylglyce

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Weigh 1.0 g of krill oil phospholipid, preheat to 50°C, add 0.1 mL of Tris-HCL buffer solution with pH 5.0;

[0039] (2) Add 1.8mL of ethanol (add in several times) and 3mL of n-hexane;

[0040] (3) Add 10 mg of phospholipase A1 to the above mixture, and start the reaction;

[0041] (4)) When at least 80% of the phospholipids in the krill oil phospholipids are hydrolyzed, the reaction is completed, cold acetone is added, and the mixture is left standing or centrifuged to obtain the lower phospholipid mixture A;

[0042] (5) Weigh 50 mg of phospholipid mixture A and 150 mg of EPA / DHA ethyl ester, and mix thoroughly to obtain oil mixture B.

[0043] (6) Preheat the oil mixture B obtained in step (4) to 30°C, add 20 mg of immobilized partial glyceride lipase Lipase G and mix, and react at a temperature of 30°C for 24 hours. After the reaction is completed, add Cold acetone, or centrifugation, to obtain the lower layer phospholipid mixture C, that is, to obtain compos...

Embodiment 2

[0046] (1) Weigh 1.0 g of krill oil phospholipid, preheat to 50°C, add 0.1 mL of Tris-HCL buffer solution with pH 5.0;

[0047] (2) Add 1.8mL of ethanol (add in several times) and 3mL of n-hexane;

[0048] (3) Add 10 mg of phospholipase A1 to the above mixture, and start the reaction;

[0049] (4) After at least 80% of the phospholipids in the krill oil phospholipids are hydrolyzed, the reaction is terminated, cold acetone is added, and the mixture is left to stand or centrifuged to obtain the lower phospholipid mixture A;

[0050] (5) Weigh 50 mg of phospholipid mixture A and 100 mg of EPA / DHA ethyl ester, and mix thoroughly to obtain oil mixture B.

[0051] (6) Preheat the oil mixture B obtained in step (4) to 30°C, add 15 mg of immobilized partial glyceride lipase Lipase G and mix, and react at a temperature of 30°C for 36 hours. After the reaction is completed, add Cold acetone, or centrifugation, to obtain the lower layer phospholipid mixture C, that is, to obtain compo...

Embodiment 3

[0054] (1) Weigh 1.0 g of krill oil phospholipid, preheat to 50°C, add 0.1 mL of Tris-HCL buffer solution with pH 5.0;

[0055] (2) Add 1.8mL of ethanol (add in several times) and 3mL of n-hexane;

[0056] (3) Add 20 mg of phospholipase A1 to the above mixture, and start the reaction;

[0057] (4) After at least 80% of the phospholipids in the krill oil phospholipids are hydrolyzed, the reaction is terminated, cold acetone is added, and the mixture is left to stand or centrifuged to obtain the lower phospholipid mixture A;

[0058] (5) Weigh 50 mg of phospholipid mixture A and 150 mg of EPA / DHA ethyl ester, and mix thoroughly to obtain oil mixture B.

[0059] (6) Preheat the oil mixture B obtained in step (4) to 30°C, add 40 mg of immobilized partial glyceride lipase Lipase G and mix, and react at a temperature of 30°C for 24 hours. After the reaction is completed, add Cold acetone, or centrifugation, to obtain the lower layer phospholipid mixture C, that is, to obtain compo...

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PUM

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Abstract

The invention discloses an EPA/DHA type lysophospholipid composition and a preparation method thereof, the composition comprises the following components: less than or equal to 25% of phospholipid PC,more than or equal to 65% of lysophospholipid LPC, and less than or equal to 10% of glycerocholine phosphate GPC, and the content of EPA/DHA in the lysophospholipid LPC is greater than 60%. The method comprises the following steps: taking phospholipid in the krill oil as a raw material, taking phospholipase A1 as a catalyst to catalyze an alcoholysis reaction of the phospholipid, ending the reaction when 80% of phospholipid substances in the krill oil are subjected to the alcoholysis reaction, adding cold acetone, performing standing or centrifuging, and taking a lower-layer phospholipid mixture; taking a phospholipid mixture obtained in the step 1 as a raw material, taking immobilized partial glyceride lipase as a catalyst, catalyzing the reaction of a byproduct GPC in the phospholipid mixture obtained in the step 1 with EPA/DHA and ester derivatives thereof, after the reaction is finished, adding cold acetone, performing standing or centrifuging, and taking the lower-layer phospholipid mixture, thereby obtaining the composition taking EPA/DHA lysophospholipid as a main component. The composition provided by the invention is rich in EPA/DHA type lysophospholipid and has an important health-care function.

Description

technical field [0001] The invention belongs to the field of lipid preparation by biocatalysis, and specifically relates to the preparation of EPA by using biological enzyme preparations as catalysts and using phospholipids in krill oil, EPA / DHA and their ester derivatives as raw materials through a two-step enzymatic method / DHA type lysophospholipid composition. Background technique [0002] Since the 1970s, scientists have begun to study the physiological effects of Omega-3 fatty acids. The results show that Omega-3 fatty acids have antithrombotic properties, lower blood lipids, and promote cardiovascular health. Necessary for the proper functioning of the system. In the brain and retina, DHA accounts for about 20% and about 35% of the total amount of fatty acids there, respectively, and mainly exists in the form of phospholipids; DHA plays an important role in the rapid development of the brain of mammals and human infants. During prenatal growth and early postnatal g...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12P13/00
CPCC12P7/6481C12P13/001
Inventor 杨继国利敏华朱东奇
Owner 大渔华创(广州)海洋生物科技有限公司
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