Combined diagnosis paper-based micro-fluidic chip and detection method

A microfluidic chip and paper-based technology, which is applied in the field of biomedical detection, can solve the problems of complex auxiliary sample injection structure and large instrument volume, and achieve the effects of saving detection time, simplifying detection steps, and great practicability

Active Publication Date: 2019-12-13
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microfluidic chip technology has promoted the miniaturization and high-throughput development of this type of instrument. Such commercial instruments combined with microfluidic chip technology have appeared in the domestic market. Complicated injection structure and other issues

Method used

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  • Combined diagnosis paper-based micro-fluidic chip and detection method
  • Combined diagnosis paper-based micro-fluidic chip and detection method
  • Combined diagnosis paper-based micro-fluidic chip and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The invention provides a combined diagnostic paper-based microfluidic chip. It is mainly composed of various functional areas of the paper base (7), and the sample feeding part (2) and the sample dividing part (6). The chip (1) is disc-shaped, and the paper base (7) and the The chip substrate (9) of the paper base (7) and the sealing film (8) on the paper base (7) have a total of three layers; a round hole is set at the center of the chip (1), and the sampling component is placed in the round hole (2), more than 2 sample processing areas (3) are uniformly and symmetrically arranged along the diameter extension line around the sample inlet part (2); each sample processing area (3) is connected to 1 at the outlet along the diameter extension line Each sampling part (6); each sampling part is connected to a detection area (5); the chip (1) is also provided with more than two screw holes (4) along the direction of the extension line of the diameter for fixing;

[0061] The...

Embodiment 2

[0069] The invention provides a detection method.

[0070] Full quantitative detection of D-dimer (D-Dimer), troponin I (cTnI), myoglobin (MYO), creatine kinase isoenzyme (CKMB), C Reactive protein (CRP), procalcitonin (PCT), the contents of a total of 6 protein markers to be detected.

[0071] The first step, preparation: choose a chip 1 with 4 sedimentation equalization pools 2-4 and detection areas 5, and each detection area 5 has 2 detection channels 11. Label quantum dots on D-dimer (D-Dimer), troponin I (cTnI), myoglobin (MYO), creatine kinase isoenzyme (CKMB), C-reactive protein (CRP), calcitonin Primogen (PCT) mouse monoclonal antibody, two by two groups are sprayed in the nanofiber hole 3-9 of quantum dot reaction channel 3-3 before a detection area 5; The anti-antibody is sprayed in the detection reaction chamber 5-1 and the quality inspection reaction chamber 5-2 of the detection channel 11 of the same detection area 5, and dried at 37 degrees. Install the sampli...

Embodiment 3

[0077] Test results

[0078] According to the detection method and steps of Example 2, the following experimental results were obtained.

[0079] The coefficients of variation of the six target markers ranged from 5.20% to 8.30%, the lowest detection limit of which was 0.26ng / mL, and the correlation coefficient r≥0.9992 within the linear range of 2.50ng / mL to 108.00ng / mL.

[0080] The test results of creatine kinase isoenzymes were tested by the same batch of whole blood samples as those in the hospital, and the experimental data were compared with the original test results of the hospital one by one. The linear correlation fitting equation is y=1.0050x+0.1098, and the correlation coefficient r = 0.9970, ie R 2 = 0.9940. It shows that there is no significant difference between the creatine kinase isoenzyme test results and the hospital test results. See Figure 11 .

[0081] The linear correlation fitting equation between C-reactive protein test results and hospital measu...

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Abstract

The invention belongs to the technical field of biomedical detection and relates to a micro-fluidic chip for detecting a human whole blood sample and a detection method. The chip is mainly composed ofvarious functional areas of a paper base (7), a sample injection component (2) and sample separation components (6). The chip is characterized in that the chip (1) is disc-shaped; the chip (1) is provide with the paper base (7), a chip substrate (9) for placing the paper base (7) and a sealing film (8) arranged on the paper base (7); a circular hole is formed in the circle center of the chip (1);the sample injection component (2) is arranged in the circular hole; more than two sample treatment areas (3) are uniformly and symmetrically arranged around the sample injection component (2) alongthe diameter extension lines of the sample injection component (2); the outlet of each sample treatment area (3) is connected with one sample separation component (6) along a diameter extension line;each sample separation component is connected with a detection area (5); and more than two screw holes (4) are formed in the chip (1) in the direction of the diameter extension lines so as to be usedfor fixation.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and relates to a microfluidic chip for detecting human whole blood samples and a detection method. Background technique [0002] The excellent fluorescent properties of quantum dots in detection make them widely used in the field of in vitro immunodiagnosis. A fast and stable detection method based on quantum dots as a signal medium has emerged - quantum dot immunofluorescence. The quantum dot immunofluorescence method is to mark the quantum dots on the detection antibody, and the complex made of quantum dot antibody is sprayed on the quantum pad in advance, and the mouse monoclonal antibody is coated on the nitrocellulose membrane to make a solid-phase antibody, that is, the detection line. Then immobilize biotin antibody on the upper end of the nitrocellulose membrane as a quality control line, the sample is dropped onto the sample pad, and flows through the quantum pad and the nit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/53G01N33/58B01L3/00
CPCG01N33/6803G01N33/6887G01N33/5302G01N33/588B01L3/502753
Inventor 廖晓玲徐文峰张圆圆黄金霞
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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