High-expression cellulose endonuclease gene as well as recombinant vector and protein thereof

A technology of cellulase endonuclease and recombinant vector, which is applied in vectors, nucleic acid vectors, genetic engineering, etc. It can solve the problems of target product inactivation, low protein yield, troublesome purification steps, etc., achieve strong biological activity and reduce costs , to achieve the effect of mass production
CN110592121AActive Publication Date: 2019-12-20HUNAN BUTIAN PHARMA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUNAN BUTIAN PHARMA
Publication Date
2019-12-20

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Abstract

The invention relates to a high-vitality cellulose endonuclease artificially synthetic gene shown in a nucleotide sequence in SEQ ID NO.1 in a sequence table or a sequence which has 90% homology or above and coding the same biological functional protein with the nucleotide sequence shown in the SEQ ID NO.1. The nucleotide sequence shown in the SEQ ID NO.1 in the sequence table can use pichia pastoris constitutive expression plasma pGAPZaA as a vector and realize high-level recombination expression of target protein in pichia pastoris host bacteria X33. Meanwhile, a screened high-level expression transformant can realize high-level secretion of recombinant protein by use of inorganic salt high-density fermentation, and by simple nickel affinity chromatography purification, a novel recombinant cellulose endonuclease protein pure product with high vitality can be obtained. The recombinant protein has very high capacity for hydrolyzing cellulose substances and producing glucose and has important application value in biological energy source, feed and food industries.
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Description

technical field

[0001] The invention belongs to the technical field of biogenetic engineering, and relates to a novel high-expression cellulase gene derived from poria cocos and its recombinant carrier and protein. Background technique

[0002] The annual output of crop straw alone in my country is as high as 6×10 8 ~7×10 8 tons, but these valuable cellulose resources have not been effectively utilized. Using cellulase to decompose cellulose is an efficient and environmentally friendly method. The use of cellulase to degrade cellulose and convert it into fuel, food and chemicals, such as sugar, ethanol, feed protein, etc., has great economic significance for alleviating the energy crisis and the shortage of food and feed resources. Cellulase will be used in food , Feed, environmental protection, energy and resource development and other fields play an important role. According to the structure of cellulase, cellulase can be divided into two categories: cellulase complex ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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