A new method and application of producing lactic acid by fermenting straw with Pyrocystida sp.

The invention relates to a technology of Trichoderma piriformis and straw, which is applied in the field of biotechnology renewable energy, can solve the problems of low technical efficiency, high pollution, high consumption and the like, and achieves the effects of simple fermentation process, improved economic benefits and low equipment requirements.

Active Publication Date: 2020-07-14
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, a large number of sorghum straw resources in rural areas of our country are completely in a state of high consumption, high pollution, low utilization rate, and low output, and sorghum straw has not been rationally developed and utilized as an energy source
Sorghum straw can be regenerated as resources through anaerobic digestion treatment, but the existing anaerobic digestion technology has problems of low technical efficiency and difficulty in popularization

Method used

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  • A new method and application of producing lactic acid by fermenting straw with Pyrocystida sp.

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Effect test

Embodiment 1

[0035] Embodiment 1, the preparation of pirocystis spirochete Piromyces CY1 inoculum

[0036]Draw 1mL of Piromyces CY1 culture and inoculate it into 9mL of anaerobic medium with air-dried and crushed sorghum stalks as the substrate in a 20mL Heinz anaerobic tube. The final concentrations of the medium were 1600IU / mL and 2000IU / mL, respectively, and the final concentration of chloramphenicol in the medium was 50 μg / mL. After anaerobic culture at 39°C for 72 hours, the growth peak is reached, and the fermentation broth is a high-activity bacterial agent at this time.

Embodiment 2

[0037] Embodiment 2, the method for producing lactic acid by fermenting straw with Piromyces CY1

[0038] 1. Method for producing lactic acid by anaerobic fermentation of sorghum stalks

[0039] 90 mL of liquid basic medium was placed in a 100 mL volume anaerobic fermentation bottle, and 1.0 g of crushed and air-dried sorghum straw was used as a substrate. Deoxygenation. Sterilize. Inoculate 10 mL of Piromyces CY1 subcultured for 72 h into the above-mentioned anaerobic medium with sorghum straw, wheat straw, corn straw and rice straw as substrates, and compound antibiotics were added at the same time to make Its final concentration in the anaerobic medium solution is 1600 IU / mL of penicillin and 2000 IU / mL of streptomycin sulfate, the final concentration of chloramphenicol in the medium is 50 μg / mL, and cultured anaerobically at 39° C. for 7 days. A total of 3 parallel experiments were set up, and the lactic acid production in the fermentation broth was measured every 24 ho...

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Abstract

The invention relates to the field of renewable energy sources of biotechnology, in particular to a method for producing lactic acid by fermenting straw with Piromyces CY1 and application thereof. Theinvention discloses a method for producing lactic acid by carrying out anaerobic fermentation on straw with the Piromyces CY1 and application thereof in preparation of lactic acid. The Piromyces CY1is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the Piromyces CY1 is CGMCC NO.18141. The Piromyces CY1 disclosed by the invention can be preserved in vitro for passage and survival. The fermented straw can produce a large amount of high-concentration lactic acid. The fermentation process is simple, low in equipment requirement and convenient to popularize, and has important industrial application value and development prospect in the industrial field.

Description

technical field [0001] The invention relates to the field of biotechnology renewable energy, in particular to a method and application for producing lactic acid by anaerobic fermentation of straw. Background technique [0002] Lignocellulose is the main component of straw, and the hydrolysis of lignocellulose is the rate-limiting step in the whole anaerobic digestion, and it is also the difficulty of the whole technology. Lignocellulosic biomass is mainly composed of cellulose, hemicellulose and lignin. The covalent bonds combined by lignin and hemicellulose embed cellulose molecules in it. The ether bonds and carbon-carbon bonds in lignin have Three-dimensional structure of high molecular aromatic compounds, these strong bonds inhibit the action of hydrolytic enzymes. Therefore, lignocellulose needs to be pretreated. The common pretreatment methods of lignocellulose include mechanical method, heat treatment method and chemical treatment, which can effectively promote anae...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12N1/14C12R1/645
CPCC12N1/14C12P7/56Y02P20/59
Inventor 魏亚琴王治业张静荣
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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