Analysis method for extracting lipids in freeze-dried food based on ultrasonic single solvent
An analytical method and freeze-drying technology, applied in the field of analysis and detection, can solve problems such as unsatisfactory lipid recovery rate, and achieve the effects of wide coverage of detected lipids, shortened extraction time, fast and accurate qualitative and quantitative
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Embodiment 1
[0036] An ultra-high performance liquid chromatography-high resolution quadrupole time-of-flight mass spectrometry analysis method based on ultrasonic single solvent extraction of lipids in vacuum freeze-dried DHA egg yolk powder, the method specifically includes the following steps:
[0037] 1) Ultrasonic single-solvent extraction of lipids in freeze-dried food: separate the egg yolk from DHA eggs provided by Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, vacuum freeze-dry to obtain solid egg yolk, grind it into powder, weigh 10mg, and add internal standard Mixture 10μL, internal standard contains: TAG 15:0 / 18:1-D7 / 15:0, Cer d18:1 / 17:0, SM d18:1 / 12:0, DAG 15:0 / 18:1- D7, FFA 16:0-D4, PC 15:0 / 18:1-d7, PE 15:0-18:1-d7, PS 15:0-18:1-d7 and PA-15:0-18: 1-d7 are 100 μg / mL, PI 15:0-18:1-d7 and PG 15:0-18:1-d7 are 10 μg / mL, CE17:0 is 1000 μg / mL; then add 5.2 mL single Solvent extraction solution - isopropanol, vortex and mix well, then ultrasonically as...
Embodiment 2
[0050] The difference between Example 2 and Example 1 is that: DHA eggs are replaced with hidden eggs as samples to be tested. Freeze-dried Tibetan egg yolk powder detected 184 lipids in positive ion mode, including 99 TAGs, 44 PCs, 23 DAGs and MAGs, 14 SMs, 4 CEs ( Figure 4A ); freeze-dried Tibetan egg yolk powder detected 218 lipids in negative ion mode, including 61 Cer, 33 FFA, 66 PE, 31 PI, 12 PG, 8 PA, 7 PS ( Figure 4A ); 12 classes of lipids were quantitatively analyzed by the added internal standard, and the content figure (mg / g) of the 12 classes of lipids detected by freeze-dried Tibetan egg yolk powder was as follows: Figure 4B As shown, the fatty acid composition and content of each lipid are shown in Table 3.
[0051] Lipid qualitative and quantitative results in the sample in Table 3
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[0065] The above data...
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