Analysis method for extracting lipids in freeze-dried food based on ultrasonic single solvent

An analytical method and freeze-drying technology, applied in the field of analysis and detection, can solve problems such as unsatisfactory lipid recovery rate, and achieve the effects of wide coverage of detected lipids, shortened extraction time, fast and accurate qualitative and quantitative

Pending Publication Date: 2019-12-20
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Methanol, butanol, MTBE and their mixtures have been used as single-phase extraction solvents for the extraction of lipids from human plasma. rate is still not ideal

Method used

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  • Analysis method for extracting lipids in freeze-dried food based on ultrasonic single solvent
  • Analysis method for extracting lipids in freeze-dried food based on ultrasonic single solvent
  • Analysis method for extracting lipids in freeze-dried food based on ultrasonic single solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] An ultra-high performance liquid chromatography-high resolution quadrupole time-of-flight mass spectrometry analysis method based on ultrasonic single solvent extraction of lipids in vacuum freeze-dried DHA egg yolk powder, the method specifically includes the following steps:

[0037] 1) Ultrasonic single-solvent extraction of lipids in freeze-dried food: separate the egg yolk from DHA eggs provided by Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, vacuum freeze-dry to obtain solid egg yolk, grind it into powder, weigh 10mg, and add internal standard Mixture 10μL, internal standard contains: TAG 15:0 / 18:1-D7 / 15:0, Cer d18:1 / 17:0, SM d18:1 / 12:0, DAG 15:0 / 18:1- D7, FFA 16:0-D4, PC 15:0 / 18:1-d7, PE 15:0-18:1-d7, PS 15:0-18:1-d7 and PA-15:0-18: 1-d7 are 100 μg / mL, PI 15:0-18:1-d7 and PG 15:0-18:1-d7 are 10 μg / mL, CE17:0 is 1000 μg / mL; then add 5.2 mL single Solvent extraction solution - isopropanol, vortex and mix well, then ultrasonically as...

Embodiment 2

[0050] The difference between Example 2 and Example 1 is that: DHA eggs are replaced with hidden eggs as samples to be tested. Freeze-dried Tibetan egg yolk powder detected 184 lipids in positive ion mode, including 99 TAGs, 44 PCs, 23 DAGs and MAGs, 14 SMs, 4 CEs ( Figure 4A ); freeze-dried Tibetan egg yolk powder detected 218 lipids in negative ion mode, including 61 Cer, 33 FFA, 66 PE, 31 PI, 12 PG, 8 PA, 7 PS ( Figure 4A ); 12 classes of lipids were quantitatively analyzed by the added internal standard, and the content figure (mg / g) of the 12 classes of lipids detected by freeze-dried Tibetan egg yolk powder was as follows: Figure 4B As shown, the fatty acid composition and content of each lipid are shown in Table 3.

[0051] Lipid qualitative and quantitative results in the sample in Table 3

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[0065] The above data...

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Abstract

The invention relates to an ultra-high performance liquid chromatography-high-resolution quadrupole flight time mass spectrometry method for extracting lipids in freeze-dried food based on an ultrasonic single solvent, which is characterized in that the lipids in the freeze-dried food are extracted by using an ultrasonic-assisted single solvent, and then positive and negative ions are quickly scanned by using ultra-high performance liquid chromatography-high-resolution quadrupole flight time mass spectrometry within 34 min to realize rapid qualitative and quantitative analysis of the lipids. The method is fast and simple to operate, the extraction is not affected by protein, the extraction time is short, the toxicity of the solvent is small, the repeatability is good, the recovery rate ofvarious lipids is high, and the purpose of high coverage of the lipids is achieved.

Description

technical field [0001] The invention relates to an analysis method for extracting lipid in freeze-dried food based on ultrasonic single solvent, and its application in qualitative and quantitative analysis of lipid in different types of freeze-dried food, belonging to the field of analysis and detection. [0002] technical background [0003] Freeze-dried food is obtained by vacuum freeze-drying technology, rapid freezing, and dehydration under vacuum. Its nutritional components have been preserved to the maximum extent, and it can be stored for a long time without adding preservatives, which has attracted the attention of researchers. As one of the three major nutrients, lipid is of great significance to human nutrition and health. The structure and composition of lipids are closely related to their nutritional and healthy qualities. Different types of lipids have different bioavailability in the human body. Therefore, it is very important to analyze the lipids in freeze-dri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/04G01N30/06G01N30/14G01N30/34G01N30/86
CPCG01N30/02G01N30/04G01N30/06G01N30/14G01N30/34G01N30/72G01N30/8696G01N2030/045G01N2030/146
Inventor 魏芳谢亚吕昕陈洪黄凤洪邓乾春郑明明
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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