Method for detecting 16 kinds of local anesthetic components in adult external products
A detection method and local anesthesia technology, applied in measuring devices, material separation, analysis of materials, etc., can solve the problems of no uniform quality standards for adult external use products, and achieve the effects of strong practicability, simple operation process, and improved sensitivity
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[0032] High performance liquid chromatography analysis method
[0033] Instruments and conditions:
[0034] Shimadzu LC 20-AT high performance liquid chromatography (with diode array detector). Methanol is chromatographically pure; phosphoric acid, dipotassium hydrogen phosphate and sodium heptanesulfonate are analytically pure. The ingredients of local anesthesia are listed in Table 1.
[0035] Table 1.16 Local Anesthetic Standard Substances
[0036]
[0037]
[0038] Chromatographic column: Agilent Poroshell 120EC-C18 (50mm×4.6mm, 2.7μm);
[0039] Flow rate: 1.5mL / min;
[0040] Column temperature: 35°C;
[0041] Injection volume: 10μL;
[0042] The wavelength range of qualitative analysis is 200-400nm, the wavelength of quantitative analysis is 210nm (lidocaine, prilocaine, ropivacaine, bupivacaine and pramoxine) and 280nm (procainamide, procaine chloroprocaine, benzocaine, buprocaine, oxybucaine, tetracaine, eugenol, dibucaine, eugenol formaldehyde and isoeugen...
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