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Temperature control device for picoliter-grade liquid

A temperature control device and level technology, applied in the direction of temperature control, non-electric variable control, control/regulation system, etc., can solve the problems of large sample size, poor portability, heavy weight, etc., and achieve small size, good portability, and sample size. small amount of effect

Active Publication Date: 2019-12-20
NORTHEASTERN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0003] Aiming at the problems of high power consumption, heavy weight, large sample size and poor portability existing in existing temperature controllers, the present invention proposes a picogram-level liquid temperature control device

Method used

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  • Temperature control device for picoliter-grade liquid
  • Temperature control device for picoliter-grade liquid
  • Temperature control device for picoliter-grade liquid

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Embodiment Construction

[0024] In order to make the above objects and advantages more comprehensible, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Apparently, the examples described in the present invention are only some examples of the present invention, not all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained in the field without creative efforts fall within the protection scope of the present invention.

[0025] The invention is a temperature control device for picoliter level liquid. The specific implementation is as follows: the system includes a C-type optical fiber microcavity unit 1 and a temperature control unit 2, and its system diagram is as follows figure 1 shown.

[0026] fiber optic microcavity unit such as figure 2 As shown, the optical fiber microcavity unit 1 is used to fill the solution to be reacted, and it is proposed to use multimode fiber A (MMF)...

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Abstract

The invention provides a temperature control device for a picoliter-grade liquid. The temperature control device comprises a C-type optical fiber micro-cavity unit and a temperature circulation unit,wherein the C-shaped optical fiber micro-cavity unit is used as a reaction container and is filled with a picoliter-grade reaction liquid; and the temperature circulation unit realizes ultrafast heating of the reaction liquid filled in the C-shaped optical fiber micro-cavity unit and temperature measurement of the reaction liquid, and further forms temperature circulation. The temperature controldevice for the picoliter-grade liquid has the advantages, superior to that of an existing temperature control system, that: the conduction of the experiment on the picoliter-grade liquid sample is realized, and the required sample volume is significantly reduced; the heating rate is ultrafast and is at a millisecond level; and the all-fiber system is small size and has good biocompatibility, portability and electromagnetic interference resistance.

Description

technical field [0001] The invention belongs to the field of optical fiber detection equipment technology and biological testing equipment, and relates to a temperature control device for picogram-level liquids. Background technique [0002] In the biological field, in some reactions, there are strict requirements on the reaction temperature of the liquid. For example, in the polymerase chain reaction (PCR), it is necessary to control the temperature of a small volume of liquid. PCR technology was invented by Kary Mullis1 in 1984 (Mullis ,K.,Faloona,F.,Scharf,S.,Saiki,R.,Horn,G.et al.Specific enzymatic amplification of DNA in vitro:the polymerase chain reaction.Cold SpringHarbSymp Quant Biol51,263-273(1986)) , used to amplify single deoxyribonucleic acid (DNA) fragments, this technique can be used for sequencing, genotyping and cloning. In 1993, Kary Mullis won the Nobel Prize in Chemistry for his invention of PCR technology. PCR is one of the most basic and important tech...

Claims

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Application Information

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IPC IPC(8): G05D23/27C12M1/38C12M1/00
CPCC12Q1/686G05D23/27C12Q2565/607C12Q2527/101
Inventor 李雪刚赵勇周雪
Owner NORTHEASTERN UNIV
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