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Separation and purification method of jujube polysaccharide with antioxidant effect

An anti-oxidation, separation and purification technology, applied in the field of food deep processing, can solve the problems of low purity and extraction rate, complex components of crude polysaccharides, limited types of polysaccharides, etc.

Active Publication Date: 2019-12-24
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polysaccharides are important bioactive substances in jujube, which have various biological activities such as immune regulation, anti-oxidation, anti-tumor, liver protection, hypoglycemia, and regulation of intestinal flora. Effective control, currently there are few studies on Jiaxian Jujube, a variety with great potential medicinal value
[0004] At present, there are many methods of separation and purification of jujube polysaccharides. However, the types of polysaccharides obtained after separation and purification are limited, and the purity and extraction rate are not high. Good Jujube Antioxidant Active Polysaccharide

Method used

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  • Separation and purification method of jujube polysaccharide with antioxidant effect
  • Separation and purification method of jujube polysaccharide with antioxidant effect
  • Separation and purification method of jujube polysaccharide with antioxidant effect

Examples

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Effect test

Embodiment 1

[0032] A preparation method for separation and purification of jujube polysaccharide, the steps are:

[0033] 1. Preparation of jujube polysaccharide:

[0034] (1) Ultrasonic-assisted hot water extraction: select the dried Jiaxian jujube, dry the jujube raw material at 50°C, and after cooling, use a high-speed pulverizer to crush the raw material, pass the crushed raw material through a 100-mesh sieve, and pass the sieve The slag in the upper part is put back into the pulverizer for re-crushing. A certain quality of jujube jujube powder sample was leached in a 70°C water bath for 30 minutes with the aid of ultrasound at a material-to-liquid ratio of 23:1, and the ultrasonic power was 420W. After centrifugal filtration, the obtained supernatant was concentrated to obtain a concentrate.

[0035] (2) Alcohol precipitation: add absolute ethanol to the concentrated solution in step (1) while stirring until the volume fraction of ethanol in the system reaches 85%, stay overnight a...

Embodiment 2

[0041] A preparation method for separation and purification of jujube polysaccharide, the steps are:

[0042] 1. Preparation of jujube polysaccharide:

[0043] (1) Ultrasonic assisted hot water extraction: select the dried Jiaxian jujube, dry the raw material of jujube at 60°C, and after cooling, use a high-speed pulverizer to crush the raw material, pass the crushed raw material through a 100-mesh sieve, and pass the sieve The slag in the upper part is put back into the pulverizer for re-crushing. A certain quality of jujube jujube powder sample was leached in a 60°C water bath for 20 minutes with the aid of ultrasound at a solid-to-liquid ratio of 23:1, and the ultrasonic power was 420W. After centrifugal filtration, the obtained supernatant was concentrated to obtain a concentrate.

[0044] (2) Alcohol precipitation: add absolute ethanol to the concentrated solution in step (1) while stirring until the volume fraction of ethanol in the system reaches 85%, stay overnight a...

Embodiment 3

[0050] A preparation method for separation and purification of jujube polysaccharide, the steps are:

[0051] 1. Preparation of jujube polysaccharide:

[0052] (1) Ultrasonic-assisted hot water extraction: select the dried Jiaxian jujube, dry the jujube raw material at 50°C, and after cooling, use a high-speed pulverizer to crush the raw material, pass the crushed raw material through a 100-mesh sieve, and pass the sieve The slag in the upper part is put back into the pulverizer for re-crushing. A certain quality of jujube jujube powder sample was leached in a 70°C water bath for 30 minutes with the aid of ultrasound at a material-to-liquid ratio of 23:1, and the ultrasonic power was 420W. After centrifugal filtration, the obtained supernatant was concentrated to obtain a concentrate.

[0053] (2) Alcohol precipitation: add absolute ethanol to the concentrated solution in step (1) while stirring until the volume fraction of ethanol in the system reaches 85%, stay overnight a...

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Abstract

The invention provides a separation and purification method of jujube polysaccharide with an antioxidant effect. The method comprises the following steps: separating and purifying the jujuba polysaccharide; the method for separating and purifying the jujube polysaccharide comprises the following steps: separating and purifying jujube polysaccharide; enabling the jujuba polysaccharide to pass through a DEAE Sepharose Fast Flow anion exchange column, sequentially carrying out gradient elution by using a PBS solution and a NaCl-PBS solution for separation, and purifying the jujuba polysaccharideby using a Sepharol S-300 gel column. According to the preparation method disclosed by the invention, three jujube antioxidant active polysaccharides, PZMP1, PZMP2 and PZMP3, can be obtained through one-time separation and purification; the three jujube antioxidant active polysaccharides obtained through separation and purification are high in purity, the purity can reach 90% or above, the yield is high, the jujube crude polysaccharides serve as the base number, the yield of PZMP1 is 2.95%, the yield of PZMP2 is 14.15%, and the yield of PZMP3 is 14.61%. In addition, the three kinds of jujube antioxidant active polysaccharides obtained through separation and purification have high oxidation resistance, raw materials are provided for antioxidant functional food, and sustainable development of jujubes is promoted.

Description

technical field [0001] The invention belongs to the field of food deep processing, and relates to polysaccharide extraction technology, and more specifically, relates to a method for separating and purifying jujube polysaccharides with antioxidative effects. Background technique [0002] Jujuba (Ziziphus Jujuba Mill.), also known as Huazao, Guanzao, and Cizao, is the fruit of the jujube tree of the Rhamnaceae Jujuba plant. In addition to water, cellulose and a variety of vitamins, red dates also contain flavonoids, triterpenoids, polysaccharides, and nucleosides. Jujube has a good health care effect and is used to treat poor appetite, fatigue, anemia and other diseases. my country is the only country in the world that has excellent jujube varieties cultivated in large areas. According to the data of the National Bureau of Statistics of the People's Republic of China, the current national jujube output is 7.3453 million tons (2014) and 6.340 million tons (2013), accounting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125
CPCC08B37/0003A23L33/125A23V2002/00A23V2200/30A23V2250/51
Inventor 冀晓龙侯春彦周晓微闫溢哲杨留枝史苗苗
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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