A kind of method and application of enriching anammox bacteria

An anaerobic ammonium oxidizing bacteria and enrichment technology, applied in the field of ammonia nitrogen treatment, can solve the problems of slow growth and long start-up time of anaerobic nitrogen oxidation reaction

Active Publication Date: 2022-07-12
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, anammox bacteria are a type of slow-growing microorganisms with a generation cycle of about 11 days. They are sensitive to light and oxygen, and have strict requirements on the living environment, resulting in a long start-up time for anaerobic nitrogen oxidation reactions.

Method used

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  • A kind of method and application of enriching anammox bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] step one:

[0052] Take the sludge at the bottom of the sedimentation tank in a domestic sewage treatment plant and let it stand for concentration, discard the supernatant, and take the bottom thickened sludge with a pre-configured inorganic salt solution (NaCl: 1000-1500 mg / L, KH 2 PO 4 : 10mg / L, CaCl 2 : 50mg / L, MgSO 4 : 300mg / L) for 4 times of panning, and after each panning, let stand for 1.5 hours to discard the supernatant to remove inorganic impurities and organic pollutants adsorbed on the sludge surface as much as possible. The elutriated and concentrated sludge is loaded into the UASB reactor, and the amount of sludge accounts for about 1 / 2 of the effective volume of the reactor.

[0053] Step 2:

[0054] Preparation of trace element nutrient solutions I and II:

[0055] Trace element nutrient solution I: EDTA 5000mg / L, FeSO 4 5000mg / L;

[0056] Trace element nutrient solution II: EDTA 5000mg / L, MnCl 2 ·4H 2 O 990mg / L, ZnSO 4 ·7H 2 O 430mg / L, CoCl ...

Embodiment 2

[0064] step one:

[0065] Take the sludge at the bottom of the sedimentation tank in a domestic sewage treatment plant and let it stand for concentration, discard the supernatant, and take the bottom thickened sludge with a pre-configured inorganic salt solution (NaCl: 1000-1500 mg / L, KH 2 PO 4 : 10mg / L, CaCl 2 : 50mg / L, MgSO 4 : 300mg / L) for 4 times of panning, and after each panning, let stand for 1.5 hours to discard the supernatant to remove inorganic impurities and organic pollutants adsorbed on the sludge surface as much as possible. The elutriated and concentrated sludge is loaded into the UASB reactor, and the amount of sludge accounts for about 1 / 2 of the effective volume of the reactor.

[0066] Step 2:

[0067] Preparation of trace element nutrient solutions I and II:

[0068] Trace element nutrient solution I: EDTA 5000mg / L, FeSO 4 5000mg / L;

[0069] Trace element nutrient solution II: EDTA 5000mg / L, MnCl 2 ·4H 2 O 990mg / L, ZnSO 4 ·7H 2 O 430mg / L, CoCl ...

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Abstract

The invention discloses a method and application for enriching anammox bacteria. By controlling the concentration of ammonia nitrogen and / or nitrite nitrogen in simulated wastewater and different hydraulic mixing conditions, the enrichment of anammox bacteria can be achieved. The cultivation is optimized in stages, which significantly improves the anammox bacteria enrichment culture and the start-up process of the anammox process, and the process start-up can usually be completed in about 3 to 4 months.

Description

technical field [0001] The invention belongs to the technical field of ammonia nitrogen treatment, and relates to a method and application for enriching anaerobic ammonia oxidizing bacteria. Background technique [0002] Nitrogen pollution is one of the important factors that cause water eutrophication, which has attracted widespread attention. The removal of ammonia nitrogen in traditional wastewater treatment is generally achieved through nitrification and denitrification processes. In the nitrification stage, ammonia nitrogen is oxidized to nitrite by ammonia oxidizing bacteria, and then nitrite is oxidized to nitrate by nitrite oxidizing bacteria; The product nitrate is converted into nitrogen by heterotrophic denitrifying bacteria to realize the denitrification of wastewater. The conversion of ammonia nitrogen into nitrate nitrogen is realized by aeration, which consumes a lot of electric energy. The denitrification process must have sufficient carbon source. In urban ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/28C02F101/16
CPCC02F3/28C02F2101/16Y02W10/10
Inventor 曹宗仑孙杰赵璞张英雄
Owner CHINA PETROLEUM & CHEM CORP
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