Method for extracting total flavonoids from aerial parts of liquorice and preparing liposomes of total flavonoids
A technology of aerial parts and total flavonoids, which is applied in the field of extracting active ingredients, can solve the problems that active ingredients cannot be well absorbed by the body, low bioavailability of total licorice flavonoids, waste of resources, etc., achieve good bioavailability, and simple preparation method , the effect of dispersion system stability
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Embodiment 1
[0025] 1. a method for extracting total flavonoids from aerial parts of Radix Glycyrrhizae, the steps are as follows:
[0026] (1) Preparation of crude extract of licorice shoots
[0027] Cut the aerial parts of licorice into small pieces and place them in an oven at 60°C to dry overnight, crush them through a 60-mesh sieve with a pulverizer, accurately weigh 100 g of the aerial parts of licorice, add 20 times the amount of 80% ethanol solution, and reflux at 85°C for 2 h, vacuum suction filtration to separate the filtrate and filter residue, repeat extraction of the medicinal residue once, and combine the two supernatants to obtain the crude extract of the aerial part of licorice.
[0028] (2) Separation of flavonoids in the extract of licorice shoots
[0029] Concentrate under reduced pressure the crude extract of the aboveground part of licorice obtained in step (1) to 1 / 10 of the original volume, add 3 times the amount of ethyl acetate solution for extraction, take the up...
Embodiment 2
[0033] A preparation method for total flavonoid liposomes of licorice shoots, the steps are as follows:
[0034] Precisely weigh 400 mg of lecithin and 100 mg of cholesterol into a pear-shaped bottle, add 20 ml of absolute ethanol as an organic solvent, ultrasonically dissolve until completely dissolved, add 5 ml of 1 mg / ml licorice shoot flavonoid solution, shake evenly until the solution The mixed solution was yellow and transparent, and the reaction solvent was removed under reduced pressure at 35°C until a yellow transparent film was formed on the inner wall of the bottle, then 40 ml of PBS solution with pH 6.8 was added, reacted in a water bath at 63°C for 30 minutes, and left for 3 hours to fully hydrate , the sample was passed through a 0.45 μm microporous membrane to obtain liposomes of total licorice flavonoids prepared by the film dispersion method, and the encapsulation efficiency was determined to be 72.33%.
[0035] Result analysis: by scanning electron microscope...
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