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Saccharomyces cerevisiae for feeds, and preparation method and application of saccharomyces cerevisiae

A technology for Saccharomyces cerevisiae and feed, applied in the field of Saccharomyces cerevisiae and its preparation, can solve problems such as hidden dangers of feed safety, unstable active ingredients, and difficulty in releasing active ingredients, so as to improve nutritional and economic value, facilitate rapid absorption, deepen The effect of egg yellowness

Active Publication Date: 2019-12-31
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in our research, we found that when the dried cells of recombinant Saccharomyces cerevisiae are made into feed without any treatment, due to the thick cell wall of the yeast, it is difficult to release the required active ingredients in animals (such as chickens, ducks), causing animals to absorb low efficiency
However, when the traditional method is used to break the wall and prepare it into feed, because most of the required active ingredients are unstable or easily oxidized, and the active substances in the dried bacteria after breaking the wall have been exposed to the environment. The conditions in the production process and storage and transportation process are relatively extensive, which will lead to a large amount of oxidative damage or deterioration, which not only causes cost loss, but also brings hidden dangers to feed safety

Method used

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  • Saccharomyces cerevisiae for feeds, and preparation method and application of saccharomyces cerevisiae
  • Saccharomyces cerevisiae for feeds, and preparation method and application of saccharomyces cerevisiae
  • Saccharomyces cerevisiae for feeds, and preparation method and application of saccharomyces cerevisiae

Examples

Experimental program
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Effect test

Embodiment 1

[0056] After the fermentation is finished, adjust the pH of the fermentation broth to 6 with citric acid, and adjust the temperature to 50° C., based on the total volume of the fermentation broth, add 0.3% enzyme preparation, and act for 2.5 hours under stirring;

[0057] The enzyme preparation is β-1,3-glucanase, β-1,6-glucanase, β-1,4-glucanase, and the mass ratio of cellulase is 5:1:0.5: 3.5;

[0058] After the enzymolysis reaction is finished, spray drying is used to obtain dry cells.

Embodiment 2

[0060] After the fermentation is finished, adjust the pH of the fermentation broth to 6 with citric acid, and adjust the temperature to 50° C., based on the total volume of the fermentation broth, add 0.3% enzyme preparation, and act for 3 hours under stirring;

[0061] The enzyme preparation is β-1,3-glucanase, β-1,6-glucanase, β-1,4-glucanase, and the mass ratio of cellulase is 3:1:1: 5;

[0062] After the enzymolysis reaction is finished, spray drying is used to obtain dry cells.

Embodiment 3

[0064] After the fermentation is finished, adjust the pH of the fermentation broth to 6 with citric acid, and adjust the temperature to 50° C., based on the total volume of the fermentation broth, add 0.3% enzyme preparation, and act for 5 hours under stirring;

[0065] The enzyme preparation is β-1,3-glucanase, β-1,6-glucanase, β-1,4-glucanase, and the mass ratio of cellulase is 1:1:3: 6;

[0066] After the enzymolysis reaction is finished, spray drying is used to obtain dry cells.

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Abstract

The invention relates to the field of feed preparation, in particular to saccharomyces cerevisiae for feeds, and a preparation method and application of the saccharomyces cerevisiae. The cell membraneof the saccharomyces cerevisiae is complete, and the cell wall of the saccharomyces cerevisiae is partially broken. The preparation method comprises the steps of after fermentation of the saccharomyces cerevisiae is completed, by using the total volume of fermentation liquid as a reference, adding 0.01-1% of an enzyme preparation, and performing action for 1-8h, wherein the enzyme preparation isselected from one or a mixture of beta-1, 3-glucanase, beta-1, 6-glucanase, beta-1, 4-glucanase, alpha-1, 6-glycosidase, cellulases, alkaline protease, chitinases and glusulase. The invention providesthe saccharomyces cerevisiae which can be used for half wall-breaking of the feeds, in accordance with the saccharomyces cerevisiae, a specific enzymolysis wall-breaking method is provided, destroying of intracellular effective substances can be effectively prevented, besides, after thalli enter animal bodies, effective components can be released out from holes, and quick absorption by animals can be facilitated.

Description

technical field [0001] The invention relates to the field of feed preparation, in particular to a brewer's yeast that can be used for feed and its preparation method and application. Background technique [0002] Saccharomyces cerevisiae (Saccharomyces cerevisiae) is a single-celled microorganism, belonging to the fungus, with strong acid resistance, and is a facultative anaerobic bacteria. Active dry yeast, yeast culture, yeast extract, yeast cell wall, etc. are all feed additives allowed in the "Catalogue of Feed Additives" (2008) issued by the Ministry of Agriculture of my country. Yeast products can effectively improve the balance of the digestive tract flora of livestock and poultry, enhance the body's immunity, and provide rich nutrients, so as to achieve multiple functions such as preventing and curing digestive tract diseases and promoting growth. It is a kind of green feed additive and has extensive research and application in the feed industry. [0003] In this f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23K10/16A23K10/14A23K50/30A23K50/10A23K50/75C12R1/865
CPCA23K10/14A23K10/16A23K50/10A23K50/30A23K50/75C12N1/16
Inventor 汪志明陆姝欢熊淑婷余超李翔宇
Owner CABIO BIOTECH WUHAN CO LTD