A method for analyzing prostate-specific antigen in serum

Abstract

Through the study of the interaction between human serum albumin (HSA) and fluorescent dye molecules, we found that HSA can make the DNA intercalation dye ethyl‑4‑[3,6‑bis(1‑methyl‑4‑vinylpyridium iodine)‑9H‑ carbazol‑9‑yl)]butanoate (EBCB) showed a marked increase in fluorescence. Based on this, the present invention discloses a prostate-specific antigen (PSA) detection reagent and kit, comprising the following steps: using the PSA nucleic acid aptamer sequence and the composition principle of triple-stranded DNA to construct a triple-strand molecular beacon (TMS) , and modified on the surface of gold nanoparticles by Au-S bonds, adding bifunctional fluorescent dye EBCB to embed molecular beacons to obtain detection probes. Fluorescence occurs rapidly when PSA is present. After adding dansylamide (DNSA), the fluorescence was further amplified. The inventive reagent and kit use endogenous HSA as a fluorescent signal amplifier, have adjustable working range, low detection limit and good detection effect. In addition, the system has good selectivity to other biomarkers in serum, does not require foreign tool enzymes or nanocatalytic systems, is cost-effective, and has original innovation and application prospects.

Description

technical field [0001] The invention belongs to the field of prostate-specific antigen analysis, and in particular relates to an analysis method for detecting prostate-specific antigen with human serum albumin as a signal amplifier. Background technique [0002] Prostate cancer is a common malignant tumor of the male genitourinary system. It is the most common malignant tumor in men in developed countries in Europe and the United States, and its mortality rate usually ranks second among male cancers. In recent years, it has become the tumor with the fastest increasing morbidity and mortality in my country, seriously threatening the health of elderly men. Prostate cancer usually has an insidious onset and slow growth, because there are no obvious symptoms in the early stage, and once clinical symptoms appear, it is often a late advanced stage. Through clinical research on the diagnosis of prostate cancer, it has been found that PSA is a specific marker of prostate cancer, so...

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Problems solved by technology

However, these methods have disadvantages, such as signal amplification process requiring tool enzymes or nanocatalytic systems, complex detection steps, high cost, and long time-consuming

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