Stem cell exosome composite preparation for improving premature ovarian failure and application of composite preparation

A compound preparation, the technology of premature ovarian failure, applied in the field of biology, can solve the problems of patients' psychological and physical distress, inability to achieve complete cure, repeated illness, etc., to improve the ovarian microenvironment, improve ovarian function, and achieve good curative effect. Effect

Inactive Publication Date: 2020-01-03
陕西中鸿科瑞再生医学研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For this disease, hormones are often taken orally or injected to help the ovaries recover ovulation function in a short per

Method used

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  • Stem cell exosome composite preparation for improving premature ovarian failure and application of composite preparation
  • Stem cell exosome composite preparation for improving premature ovarian failure and application of composite preparation

Examples

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Embodiment 1

[0035] Example 1: Extraction of exosomes from human endometrial stem cells

[0036] 1. Human endometrial stem cell culture: P5 generation human endometrial stem cells are selected, and the ratio is 2×10 5 / cm 2 Density inoculated at 75cm 2 Cell culture flask, and add DMEM / F12 medium containing 10% fetal bovine serum, at 37℃, 5% CO 2 Cultivate in an incubator for 2-3 days. When it grows to a density of 80%-85%, discard the original medium, and gently wash the cell surface twice with PBS phosphate buffer to remove cell surface debris and residual serum; The PBS solution was discarded, and 15 mL serum-free DMEM / F12 medium was added to each bottle to continue culturing for 72 hours.

[0037] 2. Collection of cell culture solution: After culturing endometrial stem cells in serum-free medium for about 48-72 hours, collect the cell culture solution aseptically, filter and sterilize it with a 0.22μm disposable filter, and store in aliquots at -20°C.

[0038] 3. Extraction of exosomes from th...

Embodiment 2

[0041] Example 2: Stem cell exosomes compound preparation improves premature ovarian failure in rats

[0042] 1. Preparation of experimental animals: Screen 40 2-month-old wistar female rats with normal estrus cycle, weighing 180±20g, after adaptive feeding for 7 days, weigh again, and randomly assign 10 rats as model control group , And the remaining 30 are modeled.

[0043] 2. Establishment of a rat model of premature ovarian failure: give rats a suspension of Tripterygium wilfordii polyglycoside tablets 50mg / kg daily for 14 days, take a smear of vaginal secretions with a cotton swab at 8:00 every day, and observe after Pap staining Changes in vaginal exfoliated cells. After the gavage, blood was collected from the tail vein of the rats to detect the levels of estradiol and follicle stimulating hormone in the blood and compare them with the modeled control group. When the continuous estrus interval was observed for 10 consecutive days, the serum estradiol level was significantl...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a stem cell exosome composite preparation for improving premature ovarian failure and an application of the composite preparation. The stem cell exosome composite preparation provided by the invention is formed by compounding human endometrial stem cell exosomes and human umbilical cord mesenchymal stem cells, human endometrial stem cells are selected as a source of the stem cell exosomes, the exosomes are collected after culture, and the exosomes and a certain amount of the highly-active human umbilical cordmesenchymal stem cells are mixed for use to maximize the function of the exosomes and the stem cells, so that the ovarian physiological function can be effectively improved, and ovarian decline can bedelayed. The invention also provides the application of the above stem cell exosome composite preparation in treating the premature ovarian failure and improving hormone secretion.

Description

Technical field [0001] The invention belongs to the technical field of biology, and specifically relates to a stem cell exosomal composite preparation for improving premature ovarian failure and its application. Background technique [0002] Premature ovarian failure, as the name implies, refers to the early decline of women’s ovarian function, which is specifically manifested as some symptoms of decreased ovarian function, such as menstrual cycle disorders, abnormal menstrual flow, reproductive tract inflammation, and a series of menopausal symptoms, such as loose skin and easy insomnia , Emotional anxiety, etc., which seriously affect women's normal life. Polycystic ovary syndrome, which is common in clinical practice, is a common cause of infertility in women of childbearing age. The main reason is that the weakened ovarian function leads to abnormal follicular development and hormone metabolism disorders. For this disease, oral or injecting hormones are often used to help th...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K35/48A61P15/00C12N5/0775
CPCA61K35/28A61K35/48A61P15/00C12N5/0665C12N5/0668A61K2300/00
Inventor 董玲娟明磊国王花
Owner 陕西中鸿科瑞再生医学研究院有限公司
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