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Extraction and separation method of anthocyanin

A separation method and anthocyanin technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., to reduce the burden of processing by-products, maximize economic benefits, and increase output

Active Publication Date: 2020-01-03
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the development of a method for efficiently extracting natural anthocyanins from deep eutectic solvents, the development of effective technologies for recovering anthocyanins from DESs solvents, and the establishment of a systematic, environmentally friendly, low-cost and efficient natural pigment extraction process can not only improve the natural anthocyanins High extraction efficiency, overcome the shortcomings of low industrial extraction yield and cumbersome process, and have important significance for reducing environmental pollution and building a green chemistry and environment-friendly society

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for extracting and separating anthocyanins, comprising the following steps:

[0037](1) 1g of fresh fruit pomace is cleaned, dried, added in an ethanol solution with a volume concentration of 50%, and ground into a slurry;

[0038] (2) According to the solid-liquid ratio (g:mL) of the pomace after washing and drying to the deep eutectic solvent is 1: 20, add 20mL (8mL, 6mL, 6mL) to the above slurry in 3 times so that the molar ratio is 1 : 2 choline chloride and citric acid, a deep eutectic solvent with a water content of 30%, mixed evenly; microwave-assisted extraction, microwave power 315W, extraction temperature 35°C, extraction time 70s, ultrasonic-assisted extraction, ultrasonic power 430W , the extraction temperature is 56°C, the extraction time is 30min, centrifuged at 5000rpm for 15min, and the supernatant is collected. The obtained supernatant is the crude anthocyanin extract, and the anthocyanin extraction rate is 75%;

[0039] (3) Add 30 g of pre-sw...

Embodiment 2

[0043] A method for extracting and separating anthocyanins, comprising the following steps:

[0044] (1) 2g fresh pomace is cleaned, dried, added in the ethanol solution of volume concentration 60%, grinds into slurry;

[0045] (2) According to the solid-liquid ratio (g:mL) of the pomace after washing and drying to the deep eutectic solvent is 1: 20, add 40mL (16mL, 12mL, 12mL) to the above slurry in 3 times, and the molar ratio is 1 : 2 choline chloride and lactic acid composition, water content is 20% deep eutectic solvent, mixed evenly; microwave-assisted extraction, microwave power 290W, extraction temperature 45 ℃, extraction time 50s, ultrasonic-assisted extraction, ultrasonic power 450W, The extraction temperature was 51°C, the extraction time was 50 minutes, centrifuged at 5000 rpm for 15 minutes, and the supernatant was collected. The obtained supernatant was the crude anthocyanin extract, and the anthocyanin extraction rate was 88%;

[0046] (3) Add 40 g of pre-swel...

Embodiment 3

[0050] A method for extracting and separating anthocyanins, comprising the following steps:

[0051] (1) 2g fresh pomace is cleaned, dried, added in the ethanol solution of volume concentration 57%, grinds into slurry;

[0052] (2) According to the solid-liquid ratio (g:mL) of the pomace after washing and drying to the deep eutectic solvent is 1: 20, add 40mL (15mL, 15mL, 10mL) to the above slurry in 3 times so that the molar ratio is 1 : 1 choline chloride and oxalic acid, a deep eutectic solvent with a water content of 30%, mixed evenly; microwave-assisted extraction, microwave power 300W, extraction temperature 40°C, extraction time 60s, ultrasonic-assisted extraction, ultrasonic power 440W, The extraction temperature was 53°C, the extraction time was 400 minutes, centrifuged at 5000 rpm for 15 minutes, and the supernatant was collected. The obtained supernatant was the crude anthocyanin extract, and the anthocyanin extraction rate was 97%;

[0053] (3) Add 80 g of pre-swe...

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PUM

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Abstract

The invention discloses an extraction and separation method of anthocyanin, and belongs to the technical field of natural pigment extraction and separation. According to the method, the drying mode isimproved, the anthocyanin in pomace is extracted with a deep-crystal eutectic solvent by combining microwave extraction and ultrasonic assistance, the effective and systematic anthocyanin extraction,separation and purification process is established, and the defects of low efficiency, residual organic solvent in product and low safety of the traditional natural anthocyanin extraction method areovercome; and the new way is provided for high-valued utilization of pomace waste, the pomace is turned into wealth, the by-product treatment burden of fruit juice processing enterprises is relieved,and great economic benefits, social benefits and ecological benefits are achieved.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of natural pigments, in particular to a method for the extraction and separation of anthocyanins. Background technique [0002] Anthocyanin is a natural pigment widely present in plants, especially fruits and vegetables. It endows different tissues and organs of plants such as roots, stems, leaves, flowers, and fruits with different colors such as purple, red, and blue. Anthocyanins belong to flavonoid polyphenolic compounds, which have good antioxidant effect due to multiple phenolic hydroxyl groups in the structure. In addition, anthocyanins also have the functions of scavenging free radicals in the body, inhibiting lipid peroxidation, preventing tumors, anti-inflammation and sterilization, reducing capillary permeability, protecting gastric mucosa, and protecting eyesight, so they are widely used in food and pharmaceutical fields. At present, most anthocyanins are extracted ...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54C07D311/62
CPCC07D311/62C09B61/00C09B67/0096
Inventor 郑洁周萍刘鹏展欧仕益李好黄才欢刘付
Owner JINAN UNIVERSITY
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