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Anisotropic structural color hydrogel film based on reduced graphene oxide doping, preparation method and applications thereof

An anisotropic, hydrogel technology, used in biochemical equipment and methods, bone/connective tissue cells, color/spectral property measurement, etc. Stability and controllability, good biocompatibility, low cost effect

Active Publication Date: 2020-01-07
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing studies have disclosed cardiomyocyte detection methods based on inverse opal hydrogels. Although they can detect cardiomyocytes conveniently and quickly, they still need to be further improved in terms of detection sensitivity and efficiency.

Method used

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  • Anisotropic structural color hydrogel film based on reduced graphene oxide doping, preparation method and applications thereof
  • Anisotropic structural color hydrogel film based on reduced graphene oxide doping, preparation method and applications thereof
  • Anisotropic structural color hydrogel film based on reduced graphene oxide doping, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of an anisotropic structural color hydrogel film based on reduced graphene oxide-methacrylated gelatin-polyethylene glycol diacrylate

[0044] (1) Firstly, colloidal crystal arrays were locally prepared on glass flakes by self-assembly of silica particles. During solution volatilization, the silica particles form tightly packed nanostructures with repeating structural units, which allow permeation of polyethylene glycol diacrylate solutions. After that, the same method was used to obtain the reduced graphene oxide sheet structure on another glass sheet. The self-assembly method is to disperse colloidal particles with the same charge on the surface in a solvent at a certain concentration. Due to the charge interaction between the particle surfaces, as the solvent evaporates, the colloidal particles are automatically arranged into a hexagonal close-packed structure. of colloidal crystals.

[0045] (2) Next, buckle the glass sheet with the colloidal...

Embodiment 2

[0049] Example 2 The anisotropic structural color hydrogel film of reduced graphene oxide-methacrylated gelatin-polyethylene glycol diacrylate is used for the detection of cardiomyocytes:

[0050] (1) Cell culture: the extracted SD rat cardiomyocytes were planted on the surface of the anisotropic structural color hydrogel film of reduced graphene oxide-methacrylated gelatin-polyethylene glycol diacrylate, the method as follows:

[0051] Firstly, the cardiomyocyte suspension is added to the orifice plate with the anisotropic structural color hydrogel film, after culturing for 20 hours, the cardiomyocytes are attached to the surface of the material, and the material is transferred to a new orifice plate, adding The DMEM / F12 medium that inhibits the growth of fibroblasts continued to be cultured. After three days of cultivation, it was replaced with ordinary DMEM / F12 medium for long-term cultivation. During the whole culture process, fresh cell culture medium was changed every ...

Embodiment 3

[0059] Example 3 Drug screening evaluation application of anisotropic structural color hydrogel film based on reduced graphene oxide-methacrylated gelatin-polyethylene glycol diacrylate

[0060] The method is basically the same as in Example 2, except that isoproterenol is added to cardiomyocytes to observe the anisotropic structural color hydrogel of reduced graphene oxide-methacrylated gelatin-polyethylene glycol diacrylate The color change of the adhesive film was evaluated by the same method as above to evaluate the influence of isoproterenol on the beating frequency and contraction force of cardiomyocytes.

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Abstract

The invention discloses an anisotropic structural color hydrogel film based on reduced graphene oxide doping, and a preparation method thereof, and applications in detection of myocardial cells. According to the invention, the grow areas of cells are controlled by using the difference of the cell adhesion rates of different hydrogel materials, and a specific pattern is copied on the surface of thematerial to achieve the functional partition of photonic crystal sensing and cell directional growth, so that the reduced graphene oxide doped hydrogel film with the anisotropic structure color is prepared; compared with the method in the prior art, the preparation method provided by the invention is simple to operate and low in cost, and does not need a large detection instrument; and the prepared anisotropic structure color hydrogel film has advantages of good biocompatibility, stable optical characteristics, flexible adjustability and efficient sensing properties, does not depend on a large detection instrument, and can realize naked eye observation of beating frequency and contractility of myocardial cells.

Description

technical field [0001] The invention relates to an anisotropic structural color hydrogel film based on reduced graphene oxide doping, a preparation method and application thereof, and belongs to the technical field of hydrogel films. Background technique [0002] The heart is one of the most important organs of vertebrates. Its main function is to provide power for blood flow, provide sufficient blood flow for various organs and tissues, supply oxygen and various nutrients, take away metabolic waste, maintain other organs, Normal metabolism and function of tissue cells. In recent years, some non-cardiovascular drugs put into clinical use may delay cardiac repolarization, increase the risk of malignant arrhythmias, and even lead to sudden death. Cardiac safety evaluation is an important factor that must be investigated before various new drugs enter clinical trials. [0003] When evaluating the cardiac safety of drugs, in vitro and in vivo experiments must be combined. At p...

Claims

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Application Information

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IPC IPC(8): C08F299/02C12N5/077G01N21/25
CPCC08F299/026C12N5/0657G01N21/25C12N2533/74C12N2533/70C12N2533/20
Inventor 赵远锦李琳杰陈卓玥邵长敏孙灵钰
Owner SOUTHEAST UNIV
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