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A kind of fish meal microbial fermentation and enzymatic hydrolysis method

A technology of microbial fermentation and fish meal, applied in fermentation, animal feed, animal feed and other directions, can solve the problem of low utilization rate of fish meal digestion, and achieve the effect of eliminating specific odor, improving quality and improving digestibility

Active Publication Date: 2022-04-08
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the digestion and utilization rate of the existing fish meal is relatively low, and the macromolecular protein in the fish meal can be hydrolyzed to produce low molecular weight peptides and free amino acids by using microbial fermentation technology, which improves the digestibility and biological activity of the product. Therefore, a The technology that can improve and increase the utilization rate and conversion rate of fishmeal protein is of great significance to improve the digestibility of feed and promote the growth of livestock and poultry

Method used

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  • A kind of fish meal microbial fermentation and enzymatic hydrolysis method
  • A kind of fish meal microbial fermentation and enzymatic hydrolysis method
  • A kind of fish meal microbial fermentation and enzymatic hydrolysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: process optimization of Bacillus subtilis fermented fish meal

[0047] 1. Optimization of solid-liquid ratio

[0048]After activation of Bacillus subtilis, 10% of the inoculum was inserted into the fishmeal medium with a solid-liquid ratio of 1:24, 1:20, 1:16, 1:12, and 1:8, and the initial pH of the fishmeal medium was adjusted to 6 at 30°C. , cultured at 200rpm, and samples were taken every 24h to determine the degree of hydrolysis, and two parallels were set up in each group. The fermentation broth was centrifuged (8000r / min, 10min) and the supernatant was taken to measure amino nitrogen (formaldehyde titration) and soluble protein (biuret method). Calculation of degree of hydrolysis (DH):

[0049] DH=free amino acid content in hydrolyzate at different times / total amino nitrogen and total protein content in fishmeal medium*100%

[0050] The results are attached figure 1 As shown, the degree of hydrolysis of the solid-liquid ratio 1:24 is the highest...

Embodiment 2

[0060] Embodiment 2: process optimization of bacillus licheniformis fermented fish meal

[0061] 1. Optimization of solid-liquid ratio

[0062] After the activation of Bacillus licheniformis, 5% inoculum was inserted into the fishmeal medium with a solid-liquid ratio of 1:20, 1:16, 1:12, 1:8, and 1:4, and the initial pH of the fishmeal medium was adjusted to 6. Cultivate at 30°C and 200 rpm, take samples every 24 hours to measure the degree of hydrolysis, and set two parallels for each group. The fermentation broth was centrifuged (8000r / min, 10min) and the supernatant was taken to measure amino nitrogen (formaldehyde titration) and soluble protein (biuret method). Calculation of degree of hydrolysis (DH):

[0063] DH=free amino acid content in hydrolyzate at different times / total amino nitrogen and total protein content in fishmeal medium*100%

[0064] The results are attached Figure 5 As shown, the degree of hydrolysis of the solid-liquid ratio 1:12 group reached the hi...

Embodiment 3

[0074] Embodiment 3: Enzymolysis and mixed fermentation after Bacillus subtilis fermentation

[0075] After activation of Bacillus subtilis, 10% of the inoculum was inserted into the fish meal medium with a solid-liquid ratio of 1:12, and divided into 5 groups. Groups 1-4 were cultured at 30°C and 200rpm for three days, then added papain, neutral Protease, alkaline protease and flavor protease 1000U / mL were reacted at 50°C and 200rpm in a water bath shaker, and samples were taken every hour to determine the degree of hydrolysis; the fifth group was sterilized after three days of fermentation, and 10% Bacillus licheniformis was added to ferment, 24h samples were taken to determine the degree of hydrolysis. There are two parallels for each group. The calculation method of the degree of hydrolysis (DH) refers to the aforementioned "optimization of solid-liquid ratio".

[0076] After testing, the degree of hydrolysis reached 40.68% on the second day of fermentation by the two ki...

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Abstract

The invention belongs to the technical field of feed and discloses a method for microbial fermentation and enzymolysis of fish meal. The microbial fermentation and enzymolysis method of fish meal of the present invention comprises the following steps: (1) inoculating Bacillus subtilis / Bacillus licheniformis into the culture medium, and activating and culturing at 25-35°C for 36-60h as seed liquid; (2) inoculating fish meal Weigh and add water to the culture bottle, and adjust the initial pH to 5-7, place it as a fish meal medium after sterilization; (3) insert the activated Bacillus subtilis / Bacillus licheniformis seed liquid into the fish meal medium , cultivated at 25-35° C., with a stirring rate of 100-300 r / min; (4) after culturing in step (3) for 66-78 hours, adding papain, flavor protease, neutral protease or alkaline protease for enzymatic hydrolysis. In the method, fermentation is performed first, and then enzymolysis is carried out, and a fish meal with high hydrolysis rate, favorable digestion and high biological activity is obtained through process optimization and strain and enzyme screening.

Description

technical field [0001] The invention relates to the technical field of feed, in particular to a method for microbial fermentation and enzymolysis of fish meal. Background technique [0002] my country's feed industry has a long history. In recent years, the increasing social demand, the rapid development of food and animal husbandry industries, and the people's strong demand for high-quality animal products have continuously stimulated the demand for high-quality protein feed. As the main nutrient in aquatic feed, protein is a key factor affecting feed cost. Most aquatic animals, especially carnivorous fish, require a higher protein content in the feed (40%-50%). Fishmeal is a high-protein feed material that uses one or more kinds of fish as raw materials, after degreasing, dehydrating, and crushing. Because it is rich in protein, omega-3 unsaturated fatty acids, and low in carbohydrates, it has good palatability and High digestion and absorption rate and other characteris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A23K10/12A23K10/14A23L5/20
CPCA23K10/12A23K10/14A23L5/20A23L5/25A23L5/28
Inventor 毛相朝孙建安孟莹
Owner OCEAN UNIV OF CHINA
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