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Preparation method of high-quality agarose

A kind of agarose, high-quality technology, applied in the field of biochemistry, can solve the problems of high production cost, low gel strength, poor agar applicability, etc., and achieve the effect of easy operation and implementation, good gelation and stable properties

Active Publication Date: 2020-01-17
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestically reported agarose extraction techniques mainly include dimethyl sulfoxide (DMSO) method, cetyl pyridinium chloride (CPC) method, sodium iodide method, ammonium sulfate method, chitin method, Levano method, polyethylene glycol method, etc. Diol method, DEAE-cellulose method, acetylation method, EDTA sodium salt method, chelation method, urea method, etc., except for the currently commonly used DEAE cellulose method, other methods have low yield and poor product color. Or low gel strength, high production costs, etc.
Although the DEAE cellulose method is a relatively mature process, DEAE cellulose has poor applicability to agar from different sources and is expensive, which limits its application range and large-scale production of agarose.

Method used

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  • Preparation method of high-quality agarose
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  • Preparation method of high-quality agarose

Examples

Experimental program
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Effect test

Embodiment 1

[0035] (1) Take 2g of agar gel, add 200ml of ultrapure water, swell for 2 hours, put it in boiling water and stir until dissolved, put it in a water bath at 60°C and keep it warm for more than 30 minutes;

[0036] (2) Add 50ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.2g of polyaluminum chloride, continue to stir for more than 30 minutes, and put it in a water bath at 60°C to keep warm for 30 minutes after the dissolution is completed;

[0037](3) Add 50ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.4g of polyacrylamide, continue to stir for more than 30 minutes, and put it in a water bath at 60°C for 30 minutes after the dissolution is completed;

[0038] (4), in a water bath at 60°C, add 50ml of polyaluminum chloride solution to 200ml of agar gel sample solution, and stir for 120min;

[0039] (5) In a water bath at 60°C, add 50ml of polyacrylamide solution to the solution that has been flocculated ...

Embodiment 2

[0042] (1) Take 4g of agar gel, add 400ml of ultrapure water, swell for 2 hours, put it in boiling water and stir until dissolved, put it in a water bath at 60°C and keep it warm for more than 30 minutes;

[0043] (2) Add 100ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.4g polyaluminum chloride and stir, and put it into a water bath at 60°C to keep warm for 30min after the dissolution is completed;

[0044] (3) Add 100ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.8g of polyacrylamide and stir, and after the dissolution is completed, put it in a water bath at 60°C for 30 minutes;

[0045] (4), in a water bath at 60°C, add 100ml of polyaluminum chloride solution to 400ml of agar gel sample solution, and stir for 120min;

[0046] (5) In a water bath at 60°C, add 100ml of polyacrylamide solution to the solution flocculated with polyaluminum chloride, stir for 120min, and filter while hot if flocs settle....

Embodiment 3

[0050] (1) Take 2g of agar gel, add 400ml of ultrapure water, swell for 2 hours, put it in boiling water and stir until dissolved, put it in a water bath at 50°C and keep it warm for more than 30 minutes;

[0051] (2) Add 100ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.4g of polyaluminum chloride into the mixture and stir to dissolve it, and keep it in a water bath at 50°C for 30 minutes;

[0052] (3) Add 100ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.8g of polyacrylamide and stir to dissolve it, and keep it in a water bath at 50°C for 30 minutes;

[0053] (4), in a water bath at 50°C, add 100ml of polyaluminum chloride solution to 400ml of agar gel sample solution, and stir for 120min;

[0054] (5) In a water bath at 60°C, add 100ml of polyacrylamide solution to the solution flocculated with polyaluminum chloride, stir for 120min, and the flocs will settle, then filter while hot.

[0055] (6) Wh...

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Abstract

The invention discloses a method for separating and preparing high-quality agarose from agar by a combined flocculation method. The method comprises the following steps: preparing an agar solution with a certain concentration, heating and dissolving; preparing a polyaluminium chloride solution with proper concentration and a polyacrylamide solution with proper concentration; keeping the temperature in a water bath kettle at 40-100 DEG C for a period of time; adding a certain volume of polyaluminium chloride solution into the stirred agar solution, and stirring for a certain time; adding a certain volume of polyacrylamide solution into the reaction solution, and stirring for a certain period of time; filtering, removing filter residues, and collecting filtrate; and cooling the filtrate to form gel, freezing and dehydrating the gel, washing, airing and crushing to obtain agarose. According to the preparation method, a polyaluminum chloride inorganic cationic flocculant and a polyacrylamide cationic flocculant are combined for use to remove the agaropectin and pigments in an agarose solution, so that the gel property is good, and the requirements of the fields of molecular biology andthe like on the high-quality agarose can be met.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a production process for extracting and preparing high-quality agarose from agar by a combined flocculant method, that is, a preparation method for high-quality agarose. Background technique [0002] Agar gum, also known as agar, frozen powder, agar, Chinese cabbage, agar powder, jelly, jelly, etc., is a kind of agar from the genus Egutonia, Gingeria, Geliflower, Gracilaria, Featherweed, and Porphyra. The substances extracted from red algae are widely used in food, medicine, daily chemical industry, bioengineering and many other fields. Agar gel is mainly a neutral sugar (containing different methyl groups) composed of an agarose skeleton, which transitions from a series of low-charged sulfate groups and pyruvic acid to agarose derivatives with high-charged and acidic substituents. A mixture, which is mainly composed of agarose and sulfur agar. [0003] Agarose, also known as agaros...

Claims

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Application Information

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IPC IPC(8): C08B37/12
CPCC08B37/0039
Inventor 张全斌鞠豪耿丽华岳洋王晶
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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