A kind of preparation method of high-quality agarose

An agarose, high-quality technology, applied in the field of biochemistry, can solve the problems of low gel strength, high production cost, poor applicability of agar, and achieve the effects of good gel properties, easy operation and implementation, and stable properties.

Active Publication Date: 2021-07-30
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestically reported agarose extraction techniques mainly include dimethyl sulfoxide (DMSO) method, cetyl pyridinium chloride (CPC) method, sodium iodide method, ammonium sulfate method, chitin method, Levano method, polyethylene glycol method, etc. Diol method, DEAE-cellulose method, acetylation method, EDTA sodium salt method, chelation method, urea method, etc., except for the currently commonly used DEAE cellulose method, other methods have low yield and poor product color. Or low gel strength, high production costs, etc.
Although the DEAE cellulose method is a relatively mature process, DEAE cellulose has poor applicability to agar from different sources and is expensive, which limits its application range and large-scale production of agarose.

Method used

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  • A kind of preparation method of high-quality agarose
  • A kind of preparation method of high-quality agarose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Take 2g of agar gel, add 200ml of ultrapure water, swell for 2 hours, put it in boiling water and stir until dissolved, put it in a water bath at 60°C and keep it warm for more than 30 minutes;

[0036] (2) Add 50ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.2g of polyaluminum chloride, continue to stir for more than 30 minutes, and put it in a water bath at 60°C for 30 minutes after the dissolution is completed;

[0037](3) Add 50ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.4g of polyacrylamide, continue to stir for more than 30 minutes, and put it in a water bath at 60°C for 30 minutes after the dissolution is completed;

[0038] (4), in a water bath at 60°C, add 50ml of polyaluminum chloride solution to 200ml of agar gel sample solution, and stir for 120min;

[0039] (5) In a water bath at 60°C, add 50ml of polyacrylamide solution to the solution that has been flocculated with polyalum...

Embodiment 2

[0042] (1) Take 4g of agar gel, add 400ml of ultrapure water, swell for 2 hours, put it in boiling water and stir until dissolved, put it in a water bath at 60°C and keep it warm for more than 30 minutes;

[0043] (2) Add 100ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.4g polyaluminum chloride and stir, and put it into a water bath at 60°C to keep warm for 30min after the dissolution is completed;

[0044] (3) Add 100ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.8g of polyacrylamide and stir, and after the dissolution is completed, put it in a water bath at 60°C for 30 minutes;

[0045] (4), in a water bath at 60°C, add 100ml of polyaluminum chloride solution to 400ml of agar gel sample solution, and stir for 120min;

[0046] (5) In a water bath at 60°C, add 100ml of polyacrylamide solution to the solution flocculated with polyaluminum chloride, stir for 120min, and filter while hot if flocs settle....

Embodiment 3

[0050] (1) Take 2g of agar gel, add 400ml of ultrapure water, swell for 2 hours, put it in boiling water and stir until dissolved, put it in a water bath at 50°C and keep it warm for more than 30 minutes;

[0051] (2) Add 100ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.4g of polyaluminum chloride into the mixture, stir and dissolve, and keep warm in a water bath at 50°C for 30 minutes;

[0052] (3) Add 100ml of ultrapure water into the beaker, and turn on the magnetic stirrer. Then add 0.8g of polyacrylamide and stir to dissolve it, and keep it in a water bath at 50°C for 30 minutes;

[0053] (4), in a water bath at 50°C, add 100ml of polyaluminum chloride solution to 400ml of agar gel sample solution, and stir for 120min;

[0054] (5) In a water bath at 60°C, add 100ml of polyacrylamide solution to the solution flocculated with polyaluminum chloride, stir for 120min, and the flocs will settle, then filter while hot.

[0055] (6) When ...

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Abstract

The invention discloses a method for separating and preparing high-quality agarose from agar in combination with a flocculation method. The method comprises the following steps: preparing an agar solution of a certain concentration, heating and dissolving; preparing a solution of polyaluminum chloride and polyacrylamide with a suitable concentration; keeping warm in a water bath at 40-100°C for a period of time; Add a certain volume of polyaluminum chloride solution and stir for a certain period of time; add a certain volume of polyacrylamide solution and stir for a certain period of time in the above reaction solution; filter, remove the filter residue, and collect the filtrate; The gel was freeze-dehydrated, washed, dried, and crushed to obtain agarose. The preparation method used in the present invention is to remove sulfur agar and pigment in the agarose solution through the combined use of polyaluminum chloride inorganic cationic flocculant and polyacrylamide cationic flocculant, which has good gel performance and can reach the fields of molecular biology and the like. Requirements for high-quality agarose.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a production process for extracting and preparing high-quality agarose from agar by a combined flocculant method, that is, a preparation method for high-quality agarose. Background technique [0002] Agar gum, also known as agar, frozen powder, agar, Chinese cabbage, agar powder, jelly, jelly, etc., is a kind of agar from the genus Egutonia, Gingeria, Geliflower, Gracilaria, Featherweed, and Porphyra. The substances extracted from red algae are widely used in food, medicine, daily chemical industry, bioengineering and many other fields. Agar gel is mainly a neutral sugar (containing different methyl groups) composed of an agarose skeleton, which transitions from a series of low-charged sulfate groups and pyruvic acid to agarose derivatives with high-charged and acidic substituents. A mixture, which is mainly composed of agarose and sulfur agar. [0003] Agarose, also known as agaros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/12
CPCC08B37/0039
Inventor 张全斌鞠豪耿丽华岳洋王晶
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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