Application of Yarrowia lipolytica in postharvest disease control of asparagus and use of storage and preservation
A technology of Yarrowia lipolytica, storage and fresh-keeping is applied in the field of postharvest disease biological control of vegetables, which can solve the problems of unseen postharvest diseases of asparagus, endanger human health, and pathogenic bacteria resistance, etc., so as to improve body immunity and reduce toxicity. , the effect of inhibiting vitality
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Embodiment 1
[0032] Screening for the concentration of Yarrowia lipolytica WSL-01 used to control the postharvest disease of asparagus;
[0033] 1. Test plan;
[0034]The tested asparagus had uniform thickness, consistent color, and no mechanical damage or pest damage on the surface. First rinse the soil on the surface of the asparagus with clean water, then soak it in 3% sodium hypochlorite solution for 5 minutes, rinse it with sterile water, and dry it at room temperature. Make a wound with a hole diameter of 3 mm and a depth of 2 mm on the tip and base of the asparagus respectively; add 20 μL of the following treatment solution to different wounds: (1) 1×10 6 cells / mL of Yarrowia lipolytica WSL-01 suspension; (2) 1×10 7 cells / mL of Yarrowia lipolytica WSL-01 suspension; (3) 1×10 8 cells / mL of Yarrowia lipolytica WSL-01 suspension; (4) 1×10 9 cells / mL of Yarrowia lipolytica WSL-01 suspension; (5) sterile saline;
[0035] After standing for 2 h, inoculate 20 μL of 5×10 4 spores / mL p...
Embodiment 2
[0042] Controlling effect of Yarrowia lipolytica WSL-01 on postharvest natural rot of asparagus;
[0043] 1. Test plan;
[0044] The tested asparagus had uniform thickness, consistent color, and no mechanical damage or pest damage on the surface. Spray 1×10 evenly on the surface of unwashed and sanitized asparagus 7 cells / mL of Yarrowia lipolytica WSL-01 suspension, and asparagus sprayed with sterile saline as a control group. After natural drying, the asparagus was sealed with plastic wrap in a plastic basket, and placed in a constant temperature and humidity incubator (humidity 90%) at 20°C for storage. Each treatment was replicated three times with 16 asparagus per replicate. The whole experiment was repeated 2 times.
[0045] The formula for calculating the disease index is as follows:
[0046] Disease index = ∑ (number of diseased plants at all levels × representative value of relative series) / (total number of plants × representative value of the highest level)
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Embodiment 3
[0050] Effects of Yarrowia lipolytica WSL-01 on the content of soluble sugar, vitamin C and chlorophyll in postharvest asparagus;
[0051] 1. Test plan;
[0052] The tested asparagus had uniform thickness, consistent color, and no mechanical damage or pest damage on the surface. Spray the following treatment solution evenly on the surface of unwashed and disinfected asparagus: (1) 1×10 7 cells / mL of Yarrowia lipolytica suspension; (2) the control group was sterile saline. After natural drying, the processed asparagus was sealed in a plastic basket with a plastic wrap, and placed in a constant temperature and humidity incubator (humidity 90%) at 20°C for storage. Each treatment was replicated three times with 16 asparagus per replicate. The whole experiment was repeated 2 times.
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