Method for determining glucosamine hydrochloride in healthcare foods

Abstract

The invention discloses a method for determining glucosamine hydrochloride in healthcare foods, and belongs to the technical field of determination of glucosamine hydrochloride. The method comprises the following steps of preparing a phenyl isothiocyanate methanol solution, a buffer solution and a glacial acetic acid aqueous solution; preparing a reference solution and a sample solution; derivingthe reference solution and the sample solution, wherein the reference solution and the sample solution are derived; performing determination, wherein chromatographic conditions include a chromatographic column of C18, 4.6*250 mm and 5 [mu]m, the detection wavelength being 254 nm, the sample injection volume being 10 [mu]L, the flow rate being 1.0 mL / min and the column temperature being the room temperature; setting a gradient elution program; and injecting the derived reference solution and sample solution into a liquid chromatograph, performing online analysis according to the chromatographicconditions, recording the chromatographic peak area and performing quantification by an external standard method. The method has the advantages of strong specificity to the glucosamine hydrochloridein healthcare products, good linearity and good reproducibility.

Description

technical field [0001] The invention belongs to the technical field of determination of glucosamine hydrochloride, in particular to a method for determination of glucosamine hydrochloride in health food. Background technique [0002] Junger Mizhiyuan Capsule is one of the health foods that can increase bone density. The main raw materials are calcium carbonate, bone collagen powder, D-glucosamine hydrochloride, casein phosphopeptide, and silicon dioxide. Glucosamine hydrochloride and calcium are the signature ingredients of this product. The scientific name of glucosamine hydrochloride is 2-amino-2 amino-deoxy-D-glucose hydrochloride, which is a stable compound of glucosamine, which is obtained by fully hydrolyzing chitin extracted from arthropod shells in hydrochloric acid solution. Its predecessor is a drug for the treatment and prevention of osteoarthritis in various joints throughout the body. By stimulating the biochemical synthesis of mucopolysaccharides and increasi...

AI Technical Summary

Problems solved by technology

[0003] The current standard determination method for amino acid glucose hydrochloride is GB / T 20365-2006 "Determination of Chondroitin Sulfate and Glucosamine Hydrochloride Content". From the perspective of the scope of application of the standard, this method is suitable for the determination of pure substances, and because glucosamine hydrochloride There is no good chromophore, and the ultraviolet absorption wavelength is only around 190nm ~ 195nm, which is at the terminal absorption, so it is easy to affect the measurement results

Health food has more complex ingredients than pharmaceuticals and contains other functional ingredients. When measured with GB / T 20365-2006 "Determination of Chondroitin Sulfate and Glucosamine Hydrochloride Content", the retention time of glucosamine hydrochloride on the C18 column is very short and there are many miscellaneous peaks , unable to meet the qualitative and quantitative requirements of the target peak

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