Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of cell-entrapped ultrathin cavity composite microfiber material

A technology of microfibers and cells, applied in the manufacture of hollow filaments, fiber chemical characteristics, cellulose/protein conjugated artificial filaments, etc., can solve problems such as shortage of organ supply, ethical disputes and constraints of immune rejection, etc., and achieve simple operation methods The effect of reliability, high efficiency and broad application prospects

Inactive Publication Date: 2020-01-24
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the prior art, organ transplantation is an ideal method for the treatment of organ failure, but problems such as organ supply shortage, immune rejection and ethical disputes seriously restrict its clinical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of cell-entrapped ultrathin cavity composite microfiber material
  • Preparation method of cell-entrapped ultrathin cavity composite microfiber material
  • Preparation method of cell-entrapped ultrathin cavity composite microfiber material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A solution containing NaCl and sodium alginate is used as the sample fluid in advance; wherein the mass volume concentration of NaCl is 0.85% g / ml, and the mass volume concentration of sodium alginate is 1% g / ml;

[0059] Methylcellulose with a mass volume concentration of 2% g / ml is used as an inert fluid;

[0060] Prepare a chitosan solution with a mass volume concentration of 4% g / ml with a volume concentration of 2% acetic acid as a modification fluid; or mix chitosan and methylcellulose to obtain methylcellulose solutions containing different chitosan concentrations as a modifying fluid;

[0061] Contains Sucrose and CaCl 2 The solution is used as a sheath fluid; the mass volume concentration of sucrose is 3% g / ml, CaCl 2 The mass volume concentration of 1% g / ml;

[0062] Using a microfluidic chip, the ultra-clean table was irradiated with ultraviolet light for more than 2 hours in advance, and then the cavity composite microfiber material embedded with cells wa...

Embodiment 2

[0066] A solution containing NaCl and sodium alginate is used as the sample fluid in advance; wherein the mass volume concentration of NaCl is 0.85% g / ml, and the mass volume concentration of sodium alginate is 2% g / ml;

[0067] Methylcellulose with a mass volume concentration of 3% g / ml is used as an inert fluid;

[0068] Prepare a chitosan solution with a mass volume concentration of 5% g / ml with a volume concentration of 2.5% acetic acid as a modification fluid; or mix chitosan and methylcellulose to obtain methylcellulose solutions containing different chitosan concentrations as a modifying fluid;

[0069] Contains Sucrose and CaCl 2 The solution is used as a sheath fluid; the mass volume concentration of sucrose is 3% g / ml, CaCl 2 The mass volume concentration of 1% g / ml;

[0070] Using a microfluidic chip, the ultra-clean table was irradiated with ultraviolet light for more than 2 hours in advance, and then the cavity composite microfiber material embedded with cells ...

Embodiment 3

[0074] A solution containing NaCl and sodium alginate is used as the sample fluid in advance; wherein the mass volume concentration of NaCl is 0.85% g / ml, and the mass volume concentration of sodium alginate is 5% g / ml;

[0075] Methylcellulose with a mass volume concentration of 5% g / ml is used as an inert fluid;

[0076] Prepare a chitosan solution with a mass volume concentration of 10% g / ml with a volume concentration of 5% acetic acid as a modification fluid; or mix chitosan and methylcellulose to obtain methylcellulose solutions containing different chitosan concentrations as a modifying fluid;

[0077] Contains Sucrose and CaCl 2 The solution is used as a sheath fluid; the mass volume concentration of sucrose is 3% g / ml, CaCl 2 The mass volume concentration of 4% g / ml;

[0078] Using a microfluidic chip, the ultra-clean table was irradiated with ultraviolet light for more than 2 hours in advance, and then the cavity composite microfiber material embedded with cells w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method of a cell-entrapped ultrathin cavity composite microfiber material, which comprises the following steps: preparing a composite microfiber material with a coating in a cavity by using a microfluidic chip; by adding cells into the central fluid, obtaining the cavity composite microfiber material with cells embedded in the cavity; and dissolving off the outer layer material, and reserving the part with molecular interaction with the inner coating to form the ultrathin wall of the microfiber, thereby obtaining the cell-entrapped ultrathin microfiber material. The operation method is simple, reliable, high in efficiency and excellent in technical effect; the internal modification coating is uniformly, stably, simply and controllably adhered to the inner cavity, so that the convenient condition is provided for the preparation of the ultrathin microfibers; and an internal cavity structure is uniform, stable, simple and controllable, and is beneficial to embedding and culture of cells. Due to the characteristics of the ultra-thin wall, the exchange of nutrient substances can be accelerated, the proliferation of cells is promoted, and a new methodand thought are provided for tissue engineering and organ regeneration.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a preparation method of an ultrathin cavity composite microfiber material loaded with cells. Background technique [0002] In the prior art, organ transplantation is an ideal method for treating organ failure, but problems such as organ supply shortage, immune rejection, and ethical disputes seriously restrict its clinical application. Therefore, tissue engineering, as a new way to construct implantable organs, has become an important development direction for the treatment of organ failure. Tissue engineering mainly uses biocompatible scaffold materials to combine with cells to prepare transplantable engineered tissues with cell functions. The purpose of organ shortage. Therefore, in the construction process of tissue engineering, it is particularly important to simulate the microenvironment of cell growth in vivo, realize the three-dimensional culture of cells...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/38A61L27/50D01F1/08D01F8/02D01F8/18
CPCA61L27/38A61L27/44A61L27/50D01F1/08D01F8/02D01F8/18C08L5/08C08L5/04C08L1/08
Inventor 秦建华刘慧王亚清
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com