Enhanced moisturizing cosmetic composition
A technology of cosmetic composition and birch sap, applied in cosmetics, cosmetic preparations, dressing preparations, etc., can solve the problem that sodium hyaluronate is difficult to meet the needs of consumers for moisturizing products
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Embodiment 1
[0033] Example 1 : Preparation of compositions A, A1, B, B1, B2, B3, B4, C, C1, C2, D, D1, D2, E, E1, E2, F, F1, F2 and J
[0034] The specific formula of each composition is as follows:
[0035]
[0036]
[0037] Note: The data in the table represent the weight % of each component, based on the total weight of the composition, the same below
[0038] Each of the above compositions was prepared as follows:
[0039] 1. Input the fresh birch sap stock solution purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. into the low-temperature drying equipment, cool down to -65°C, vacuumize to 0.1Pa, and concentrate to 1.2 times, 4 times, and 9 times;
[0040] 2. Mix the raw materials No. 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 to obtain the corresponding composition.
Embodiment 2
[0041] Example 2 : Composition A, A1, B, B1, B2, B3, B4, C, C1, C2, D, D1, D2, E, E1, E2, F, F1, F2 to keratin transglutaminase TGM1, epidermis compact Effects of gene expression of connexins (ZO-1 and CLDN1), filaggrin FLG and aquaporin AQP3
[0042] Experimental equipment: fluorescence quantitative PCR instrument (Roche), ultra-clean workbench (Su Jing), carbon dioxide incubator (Binder), microplate reader (BIO-TEK), micro-shaker.
[0043] Experimental reagents and consumables: human primary keratinocytes, 6-well plate, keratinocyte culture medium, RNA extraction kit, reverse transcription kit, Trizol lysis solution, etc.
[0044] The steps of fibroblast-based gene expression analysis are as follows:
[0045] (1) Seeding: Seed cells in a 6-well plate at a seeding density of 5E5 / well at 37°C and 5% CO 2 Incubate overnight in an incubator;
[0046] (2) Administration: When the cell plating rate in the 6-well plate reaches about 60%, the test substances of each group are a...
Embodiment 3
[0053] Example 3 : Effects of compositions B1, B2 and C1, C2 on protein expression of keratin-transglutaminase TGM1, epidermal tight junction proteins (ZO-1 and CLDN1), filaggrin FLG and aquaporin AQP3
[0054] In this example, the effects of the above-mentioned compositions B1, B2 and C1, C2 on the expression of aging-related proteins were further tested and compared.
[0055] Experimental equipment: ultra-clean workbench (Su Jing), plate washer (BIO-RAD), microplate reader (BIO-TEK), carbon dioxide incubator (Binder)
[0056] Experimental reagents and consumables: human primary keratinocytes, 12-well plates, keratinocyte culture medium, ELISA detection kits with different indicators, etc.
[0057] The test steps are as follows:
[0058] (1) Inoculation: inoculate in a 12-well culture plate at an inoculation density of 2E5 / well, culture in a 37°C and 5% CO2 incubator, and change the medium every two days;
[0059] (2) Administration: when the cell fusion reaches more than...
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