Microballons/nanometer yarn composite bracket loaded with exosomes and growth factors and preparation method of microballons/nanometer yarn composite bracket

A composite scaffold and growth factor technology, applied in prosthesis, tissue regeneration, medical science, etc., can solve the problems of low drug loading and unsatisfactory solubility of anti-fibrosis drugs, and achieve good biocompatibility and good mechanical properties Performance, the effect of promoting tissue repair

Inactive Publication Date: 2020-02-07
DONGHUA UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0006] The research group's previous research on drug-loaded nano-yarns was only nano-yarns loaded with anti-fibrosis drugs, and in the experimental results, there was only a discontinuous core structure in the transmission electron microscope i

Method used

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  • Microballons/nanometer yarn composite bracket loaded with exosomes and growth factors and preparation method of microballons/nanometer yarn composite bracket
  • Microballons/nanometer yarn composite bracket loaded with exosomes and growth factors and preparation method of microballons/nanometer yarn composite bracket
  • Microballons/nanometer yarn composite bracket loaded with exosomes and growth factors and preparation method of microballons/nanometer yarn composite bracket

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[0032] Example 1

[0033] (1) Centrifuge the culture supernatant of canine adipose stem cells (ADSC, obtained from Shanghai Sixth People’s Hospital) at 250×g for 15 minutes, and then wash the pellet with PBS 3 times and perform a low speed (300×g, 10 minutes, 2,000×g, 10 minutes) continuous centrifugation to remove cell debris. Then, the supernatant was filtered through a 0.22 μm filter. The filtered supernatant was transferred to a 15mL Amicon Ultra-15 centrifugal filter and centrifuged at 4,000×g. The ultrafiltration liquid was washed 3 times with PBS, and then ultrafiltration was performed again at 4,000×g. In order to purify exosomes, transfer the ultra-filtered liquid to Ultra-Clear TM The tube was ultracentrifuged at 100,000×g for 70 minutes to pellet the exosomes, and then washed with a large amount of PBS, and ultracentrifuged again at the same speed for 70 minutes. All steps are performed at 4°C. The exocytosis was resuspended in sterile PBS and stored in a refrige...

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Abstract

The invention relates to a microballons/nanometer yarn composite bracket loaded with exosomes and growth factors and a preparation method of the microballons/nanometer yarn composite bracket. The composite bracket is prepared by that the microballons are adhered to the surface of nanometer yarn, wherein the nanometer yarn is nanometer yarn adopting a core/shell structure; and the microballons arepolylactic acid glycolic acid copolymer microballons of which the inner parts are loaded with fibroblast growth factors. The microballons/nanometer yarn composite bracket disclosed by the invention has high stretch mechanical property and excellent biocompatibility; the structure of the nanometer yarn has larger aperture; tissue repair can be promoted by the exosomes loaded by a fiber core layer,and three-dimensional infiltration of urinary tract relevant cells is facilitated; and the microballons can release fibroblast growth factors, so that the microballons/nanometer yarn composite bracketcan be applied to fields of tissue engineering urethra and the like.

Description

Technical field [0001] The invention belongs to the field of urethral stents and their preparation, in particular to a microsphere / nano yarn composite stent loaded with exosomes and growth factors and a preparation method thereof. Background technique [0002] Urethral stricture and hypospadias are common clinical diseases in urology, which seriously affect the urination function and quality of life of patients. Due to the lack of ideal urethral replacement grafts, patients’ autologous tissues are often used for urethral replacement in clinical practice. However, this method causes huge trauma to the patient’s extraction site, and urethral fistula and restenosis are prone to occur after transplantation, so urethral stricture and urethra The repair and reconstruction of hypospadias has always been a difficult problem in clinical treatment of urology. In recent years, the development of tissue engineering and regenerative medicine has provided new directions for urethral repair. ...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/24A61L27/50A61L27/54A61L27/56A61L27/58
CPCA61L27/18A61L27/24A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/258A61L2300/414A61L2430/22C08L67/04
Inventor 莫秀梅朱晶晶朱同贺吴晶磊张楷乐
Owner DONGHUA UNIV
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