A method and device for generating an ultrasonic radiation force field for engineering cartilage cultured in vitro

Abstract

The invention provides a method and a device for generating an ultrasonic radiation force field for culturing engineering cartilage in vitro. The device of the invention includes an ultrasonic transducer, a connecting frame, a lifting platform, a fixed base, a guide ring, an engineering cartilage culture device in vitro, an ultrasonic transmitting and receiving device, a signal acquisition and processing module and an oscilloscope. The method of the present invention firstly adjusts the built-in gear of the lifting platform to control so that the emitting head of the ultrasonic transducer is immersed in the culture medium of the engineering cartilage in vitro culture device. Determine the focal length of the emitter of the ultrasonic transducer in the culture medium through the signal acquisition and processing module and the oscilloscope, so that the engineered cartilage is at the focal point of the emitter of the ultrasonic transducer; determine the direction of the ultrasonic radiation force field by rotating the ultrasonic transducer; Finally start the ultrasonic transmitting and receiving device to excite the ultrasonic transducer. The ultrasonic radiation force field generated by the method and device of the invention can mechanically regulate the engineered cartilage cultured in vitro to obtain normal engineered cartilage with good biological properties.

Description

technical field [0001] The invention relates to the fields of ultrasonic regulation, articular cartilage repair and biomedical tissue engineering, and specifically relates to a method and device for generating an ultrasonic radiation force field for culturing engineered cartilage in vitro. Background technique [0002] Normal cartilage is an indispensable tissue in joint movement. However, with the development of modern technology and changes in work and living habits, more and more people tend to maintain a certain movement posture for a long time, and articular cartilage damage is very prone to occur. At the same time, articular cartilage has poor self-healing ability and cannot regenerate. After injury, it is easy to cause problems such as joint stiffness and aggravated pain, which seriously affects the quality of life of patients. The current treatment options for articular cartilage injury include joint lavage, microfracture surgery, periosteum transplantation and cart...

AI Technical Summary

Problems solved by technology

When engineering cartilage is cultured statically in vitro, it is easy to form a hollow phenomenon of engineered cartilage, and the mechanical properties of the final engineered cartilage are insufficient.

However, most existing bioreactors can only achieve constant shear force and dynamic loading pressure.

The combined method of the two forces is relatively simple, which is not conducive to the cultivation of articular cartilage with good structure and function

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