A pretreatment method for detecting aflatoxin in food matrix
An aflatoxin and food technology, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of using a large amount of organic solvents, high analysis costs, cumbersome and time-consuming, saving organic solvents, speeding up the reaction rate, and increasing the washing rate. The effect of de-efficiency
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Embodiment 1
[0030] The detection of aflatoxin B1 and B2 in the embodiment 1 peanut
[0031] Pretreatment method one: the pretreatment method of the present invention. Specific steps are as follows:
[0032] a) Sample extraction: Weigh 1g peanut sample, place it in 4mL alkaline potassium hydroxide aqueous solution with pH ≥ 12 after homogenization, vortex shake for 30min, centrifuge at 6000r / min for 3min to separate layers, and obtain the extract;
[0033] b) In situ derivatization-solid phase dispersion microextraction: Take 40mg of purified material (MAX extraction filler (ANPEL LaboratoryTechnologies (Shanghai) Inc.P / N: SBEQ-CA3300)), add 600μL of extraction solution, vortex shake and centrifuge to separate layers , discard the supernatant; successively wash with 600 μL of acetonitrile / water (10 / 90 (v / v)) containing 0.1% acetic acid (v / v), vortex shake and centrifuge to separate layers, and discard the supernatant ; Wash with 600 μL of acetonitrile / water (40 / 60 (v / v)), vortex shake an...
Embodiment 2
[0036] The pretreatment of the aflatoxin in embodiment 2 corn
[0037]a) Sample extraction: Weigh 3 parts of 1g blank corn matrix without aflatoxin, homogenize them respectively, and test the blank matrix with aflatoxin (G1 , B1, G2, B2) spiked treatment, respectively placed in 5mL alkaline sodium hydroxide aqueous solution with pH ≥ 12, vortex shaked for extraction for 20min, centrifuged at 4000r / min for 5min to separate the layers, and obtained the extract;
[0038] b) In situ derivatization-solid phase dispersion microextraction: take 40mg of purified material (MAX extraction filler), add 800μL extract solution, vortex shake and centrifuge to separate layers, discard the supernatant; (v / v) acetonitrile / water (15 / 85 (v / v)) for washing, vortex shaking and centrifugation for layers, discard the supernatant; 800 μL of acetonitrile / water (50 / 50 (v / v)) Wash, vortex shake and centrifuge to separate layers, discard the supernatant; finally use 800 μL of acetonitrile / trifluoroaceti...
Embodiment 3
[0043] The detection of aflatoxin B1 and B2 in the vegetable oil of embodiment 3
[0044] a) Sample extraction: Weigh 1g of peanut oil sample and place it in 6mL of alkaline calcium hydroxide aqueous solution with pH ≥ 12, extract by vortex shaking for 40min, and centrifuge at 5000r / min for 4min to separate the layers to obtain the extract;
[0045] b) In situ derivatization-solid phase dispersion microextraction: Take 50mg of purified material (MAX extraction filler), add 1200μL extract, vortex shake and centrifuge to separate layers, discard the supernatant; sequentially use 1000μL containing 0.3% acetic acid (v / v) acetonitrile / water (30 / 70 (v / v)) for washing, vortex shaking and centrifugation for layers, discard the supernatant; 1000 μL of acetonitrile / water (60 / 40 (v / v)) Wash, vortex shake and centrifuge to separate layers, and discard the supernatant; finally use 1000 μL of acetonitrile / trifluoroacetic acid (5 / 1 (v / v)), place it on a constant temperature mixer at 40°C for...
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