A kind of preparation method of core-shell structure micro-nano fiber

A technology of micro-nanofiber and shell structure, applied in the direction of chemical characteristics of fibers, textile and papermaking, conjugated synthetic polymer rayon, etc., can solve the problem of low mechanical strength and modulus of extracellular matrix electrospun fibers, limited application and other problems to achieve the effect of enhancing cytocompatibility and biological activity and facilitating adhesion

Active Publication Date: 2021-10-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical strength and modulus of electrospun fibers of extracellular matrix are low, which limits their application in tissue engineering.

Method used

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  • A kind of preparation method of core-shell structure micro-nano fiber
  • A kind of preparation method of core-shell structure micro-nano fiber

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preparation example Construction

[0022] The preparation method of the core-shell structure micro-nanofiber of the present invention relates to the preparation method of the polymer synthetic material / acellular matrix core-shell structure micro-nanofiber, which needs coaxial electrospinning technology, so the preparation method of the present invention needs to use coaxial needle.

[0023] In addition, the materials required by the present invention include: animal-derived decellularized matrix, polymer synthetic materials, fluorine-containing polar reagents, and the like.

[0024] The preparation method of the present invention is as follows:

[0025] (1) Preparation of acellular matrix powder.

[0026] Take fresh animal tissues and organs (animal species can be pigs, cattle, sheep and other poultry, tissues and organs include spinal cord, peripheral nerves, small intestinal mucosa, skin, etc.), cut into 1×1×1mm 3 ~2×2×2mm 3 Cubes were soaked in PBS solution containing 1% penicillin / streptomycin to sterili...

Embodiment 1

[0037] Example 1 Preparation of PCL / PDSM core-shell structure micro-nanofibers

[0038] (1) Preparation of acellular matrix powder from porcine upper layer skin.

[0039] Take fresh pig-derived upper skin, cut into 2×2×2mm 3 Cube, soaked in PBS solution containing 1% penicillin / streptomycin and sterilized overnight; the next day, it was washed 3 times in deionized water, each time for 5 minutes, and then soaked in 3% tritonX-100 for 12 hours; the tissue was taken out and deionized Wash with water for 3 times, each time for 15 minutes, and then put into 4% sodium deoxycholate solution for 24 hours to prepare the decellularized matrix (DCM) of animal tissue.

[0040] Wash the acellular matrix (DCM) of animal tissue prepared above with deionized water for 15 min×3 times, and freeze-dry in vacuum for 2-3 days; degrease the freeze-dried DCM with dichloromethane / ethanol (2:1) After soaking for 12 hours×2 times, wash with deionized water for 15 minutes×3 times, vacuum filter overni...

Embodiment 2

[0055] Example 2 Preparation of PCL / DSCM core-shell structure micro-nanofibers

[0056] (1) Preparation of porcine spinal cord acellular matrix powder.

[0057] Take fresh porcine spinal cord tissue, cut into 1×1×1mm 3 Cube, soaked in PBS solution containing 1% penicillin / streptomycin and sterilized overnight; the next day, it was washed 3 times in deionized water, each time for 5 minutes, and then soaked in 3% tritonX-100 for 12 hours; the tissue was taken out and deionized Wash with water for 3 times, each time for 15 minutes, and then put into 4% sodium deoxycholate solution for 24 hours to prepare the decellularized matrix (DCM) of animal tissue.

[0058] Wash the acellular matrix (DCM) of animal tissue prepared above with deionized water for 15 min×3 times, and freeze-dry in vacuum for 2-3 days; degrease the freeze-dried DCM with dichloromethane / ethanol (2:1) After soaking for 12 hours×2 times, wash with deionized water for 15 minutes×3 times, vacuum filter overnight, a...

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Abstract

The preparation method of core-shell structure micro-nanofibers of the present invention relates to the preparation method of macromolecule synthetic material / decellularized matrix core-shell structure micro-nanofibers, which requires coaxial electrospinning technology, so the preparation method of the present invention needs to use coaxial needle. In addition, the materials required by the present invention include: animal-derived decellularized matrix, polymer synthetic materials, fluorine-containing polar solvents, and the like. A method for preparing micro-nano fibers with a core-shell structure of the present invention, comprising: step 1, preparing animal-derived acellular matrix powder; step 2, dissolving the acellular matrix powder and polymer synthetic material in step 1 separately, Obtain the electrospinning solution of the acellular matrix and the polymer synthetic material; step 3, use a coaxial needle to perform electrospinning on the acellular matrix and the electrospinning solution of the polymer synthetic material in step 2 to obtain the core-shell structure micronano fiber.

Description

technical field [0001] The invention relates to a preparation method of a core-shell structure micro-nano fiber. Background technique [0002] Electrospinning technology is a simple and fast preparation technology for micro-nano fibers, while coaxial electrospinning is a new method developed on the basis of traditional electrospinning technology. Traditional electrospinning is usually used to prepare micro-nano fibers of a single material. The disadvantages of the obtained fibers are relatively single components and mechanical properties, lack of functionality and specificity of the surface, and difficulty in controlling the degradation rate. Coaxial electrospinning technology uses coaxial electrospinning needles and two channels with controllable solution flow rates to continuously prepare micro-nano fibers with a core-shell structure. The resulting fibers are characterized by many components, strong comprehensive performance and wide application range. Guang et al. This ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F8/18D01F8/14
CPCD01F8/14D01F8/18
Inventor 全大萍邓荣力李锐吴嘉辉张弛陈诗浩杜钊夷白莹
Owner SUN YAT SEN UNIV
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