A Novel Enzyme-Free Glucose Sensor for In Situ Detection in Fermentation Process

Abstract

The invention discloses a novel non-enzymatic glucose sensor applied to in-situ detection of fermentation process, which can detect the concentration of glucose in fermentation liquid. It includes the following contents: Au@Ni / C nanocomposites with core-shell structure are prepared by the method of high temperature oleylamine reduction, and a working electrode is prepared with this method, and a three-electrode detection system is constructed. The electrical signal is used to obtain the data corresponding to the glucose concentration, and the glucose concentration is obtained through calculation. Compared with the existing enzyme glucose biosensor, the sensor of the present invention does not need complicated immobilization technology; high temperature resistance, can be used for in-situ detection; high precision, catalytic activity is not affected by fermentation environment such as temperature and humidity; strong stability, It can be applied to the long-term fermentation process online detection environment. Compared with the existing non-enzyme glucose biosensor, the sensor of the invention works at a lower working voltage and has high selectivity; it is not affected by the adsorption of chloride ions abundantly present in the fermentation broth, and has strong resistance to poisoning. Therefore, it becomes an enzyme-free electrochemical sensor for in-situ glucose detection in fermentation process.

Description

technical field [0001] The invention belongs to the field of in-situ glucose detection in the fermentation process, and relates to a preparation method of an electrochemical sensor for glucose detection, in particular to a preparation method of an enzyme-free glucose biosensor with a gold-nickel core-shell structure. Background technique [0002] The quantitative detection of glucose plays a vital role in the chemical industry, clinical medicine, fermentation process, and food safety. Glucose sensors account for 85% of the biosensor market. During the fermentation process, glucose is the main carbon source for cell growth and product synthesis, and its concentration in the fermentation broth must be controlled. [0003] Nowadays, most fermentation factories use enzyme-based sensors to measure glucose concentration offline, or through indirect evaluation methods such as pH value and dissolved oxygen. The reason is that in order to prevent bacterial contamination during fermen...

AI Technical Summary

Problems solved by technology

However, for the detection of glucose during the fermentation process, the glucose sensor using Au electrodes is susceptible to adsorption poisoning by oxidized intermediate substances and abundant chloride ions in the test solution.

Nickel is a commonly used metal material for the preparation of enzyme-free sensors. Nickel-based sensors have much higher electrocatalytic activity, anti-poisoning and stability than other metal sensors. (vs SCE), many impurities in the fermentation broth can be catalyzed and oxidized at this high voltage to generate a current response, resulting in a decrease in the detection accuracy of the sensor

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