Microbial agent for treating black and odorous water body in complicated closed conduits and application thereof

A technology of microbial agent and black and odorous water body, which is applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, to achieve the effect of improving water quality, eliminating black and odorous water, and improving water quality

Active Publication Date: 2020-02-14
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems, the present invention provides a microbial bacterial agent for treating black and odorous water bodies in complex culverts, and uses the microbial agent in combination with a micro-nano bubble generating device loaded with biologically active substances and conducts reasonable calculations to arrange micro-nano bubbles in the culvert Method for repairing black and odorous water body in culvert with generating device

Method used

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  • Microbial agent for treating black and odorous water body in complicated closed conduits and application thereof
  • Microbial agent for treating black and odorous water body in complicated closed conduits and application thereof
  • Microbial agent for treating black and odorous water body in complicated closed conduits and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of embodiment 1 microbial bacterial agent

[0047] To make seed liquid, each strain was inoculated into a liquid medium at 37°C and cultured at 200r / min for 24 hours to obtain a seed liquid. The liquid medium was: 3g of beef extract, 10g of peptone, 5g of NaCl, 1L of distilled water, and adjusted the pH to 7.5, sterilized at 121°C for 20 minutes.

[0048] Fermentation production: inoculate various seed liquids into the fermentation medium according to the volume ratio of 5%, and the fermentation medium is 5wt% molasses, 2wt% glucose, 2.5wt% soybean meal, and the balance is pure water. The ventilation volume is 0.2vvm, the pH is 7-8, and the microbial inoculum is obtained by fermentation at a rotating speed for 7 days.

[0049] Take Bulgarian milk according to the weight ratio of Lactobacillus bulgaricus: Streptococcus faecalis: Lactobacillus plantarum: Bacillus licheniformis: Bacillus subtilis: Bacillus natto ADT: yeast = 2:2:1:1:2:2:1 Bacillus, Strept...

Embodiment 2

[0054] Preparation and application of embodiment 2 microbial bacterial agent

Embodiment approach

[0055] A certain river in Ningbo has an average width of about 6m, a water depth of 2-2.5m, and a total length of 1300m. It mainly collects domestic sewage and rainwater, with a daily flow rate of about 5000m³ (in the case of no rainfall), and the main body of the river is a culvert structure. The present invention is used to control the black and odor of this river to improve water quality. The design and implementation plan is as follows:

[0056] will be like figure 1 The micro-nano bubble generating device (the structure of the device is described in the document with application number 201920409878.9) is placed in the sewage, and the one-way valve A, the water pump 1, and the intake pipe 2 are electrically connected to the compressed air machine. The water tank 4 starts to carry out coarse filtration of sewage due to the pressure, and the coarsely filtered water is stored in the water tank 4 , the water pump 1 is turned on, and the coarsely filtered water is pumped into t...

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Abstract

The invention belongs to microbial water body restoration, and particularly relates to a microbial agent for treating a black and odorous water body in complicated closed conduits and application thereof. The microbial agent is prepared by mixing 15 to 30 weight parts of enterococcus faecalis, 5 to 10 weight parts of lactobacilli, 25 to 50 weight parts of bacilli and 5 to 10 weight parts of saccharomycetes through fermentation. The compound efficient microbial agent and micro-nano bubbles synchronously and gradually flow from branch streams to trunk streams of the closed conduits; in the process, bioactive substances, oxygen bubbles and riverway suspended matters or suspended bottom mud are sufficiently mixed; mixtures are fast adsorbed onto suspended particles; pollutants of the water body are degraded through the biological metabolism process; and the blackness and the odor of the water body are reduced, so that the quality of the water in the complicated closed conduits is effectively improved.

Description

technical field [0001] The invention belongs to the restoration of microbial water bodies, and in particular relates to a microbial bacterial agent for treating complex black and odorous water bodies in dark drains and its application. Background technique [0002] The rapid development of social economy has brought serious negative environmental problems. The world is facing various pollutants and pollution problems from various development activities. The surge in the world's population has led to an increase in the area of ​​water pollution. Monitoring discharges to natural water flow and quality demonstrates the need for different approaches to address water quality challenges from each region. The original degradation capacity of the river is far less than the amount of pollutants discharged, and it is in an overloaded state. Not only can the pollutants not be degraded in time, but the aerobic degradation of a large amount of pollutants also consumes the dissolved oxy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/225C12R1/01C12R1/25C12R1/10C12R1/125C12R1/07C02F103/06
CPCC02F3/347C02F3/348C02F2103/06C12N1/16C12N1/20C12N1/205C12R2001/07
Inventor 刘宗丽李佳霖王勇焦绪栋林福明秦松郭小雅李长振张健郭静静
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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