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Preparation method of equol-rich biological feed additive for improving fat content in livestock muscles

A technology of biological feed and fat content, applied in the direction of animal feed, animal feed, bacteria used in food preparation, etc., can solve the problems of low conversion efficiency and lack, and achieve the effect of increasing fat content, promoting fat content, and multi-benefit

Inactive Publication Date: 2020-02-21
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of the low conversion efficiency of existing daidzein into equol and the lack of biological feed additives for increasing the fat content in muscle, and to provide a kind of equol-rich equol for improving the fat content in livestock muscle. The preparation method of the biological feed additive of horse phenol

Method used

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  • Preparation method of equol-rich biological feed additive for improving fat content in livestock muscles
  • Preparation method of equol-rich biological feed additive for improving fat content in livestock muscles
  • Preparation method of equol-rich biological feed additive for improving fat content in livestock muscles

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specific Embodiment approach 1

[0016] Embodiment one: a kind of preparation method of the equol-rich biological feed additive that is used to improve the fat content in the livestock muscle of this embodiment: one, soybean powder and alfalfa grass powder are according to mass ratio (5-9): The ratio of 3 is mixed to make fermentation medium dry powder, and a solvent is added to prepare a liquid fermentation medium with a concentration of 15-20% of the mass percentage of the fermentation medium dry powder; the solvent is a sodium butyrate aqueous solution with a concentration of 0.10-0.15mmol / L;

[0017] 2. Inoculate Bacillus subtilis into the liquid fermentation medium, ferment aerobically for 20-26 hours, then in situ heat up to 85-90°C for 0.3-1 hour sterilization, and then inoculate Bifidobacterium animalis lactic acid subspecies and Leuconostoc enterococcus Subspecies enterica, anaerobically fermented for 24-36 hours, and then sterilized at 85-90°C for 0.3-1 hour in situ to obtain a fermentation product r...

specific Embodiment approach 2

[0022] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the mesh number of soybean flour is 80-100 mesh. Others are the same as the first embodiment.

specific Embodiment approach 3

[0023] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the preparation method of alfalfa grass powder is as follows: cut the fresh grass of alfalfa into 2cm-long sections, and use a dryer at no less than 800 ° C. Dry it under the hood, reduce the water content to no more than 12%, and pulverize it with a feed mill with a mesh number of 100 mesh. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a preparation method of an equol-rich biological feed additive for increasing the fat content in livestock muscles. The invention relates to a preparation method of an equol-rich biological feed additive for increasing the fat content in livestock muscles. The invention aims to solve the problems that the conversion efficiency of converting daidzein into equol is low and biological feed additives for improving the fat content in muscles are lacked in the prior art. The method comprises the following steps: 1, mixing soybean meal and alfalfa meal to prepare a fermentation medium dry powder, and adding a solvent to prepare a liquid fermentation medium; and 2, inoculating bacillus subtilis into the liquid fermentation culture medium, performing fermentation, inoculating Bifidobacterium animalis subsp. Lactis and Leuconostoc mesenteroides subsp. Mesenteroides, performing fermentation, and adding palmitic acid and oleic acid. The conversion efficiency of the fermentation final product for converting daidzein into equol reaches 60% or above, and the fat content in muscles can be remarkably increased. The method is applied to the field of biological feed additives.

Description

technical field [0001] The invention relates to a preparation method of an equol-rich biological feed additive for increasing fat content in livestock muscle. Background technique [0002] The fat content in muscle, including intermuscular fat and intramuscular fat, has a great influence on the nutritional characteristics, state (tenderness, juiciness, etc.), flavor, taste and processing characteristics of muscle, and is an important indicator of livestock and poultry meat quality. Livestock ketone bodies rich in intermuscular fat and intramuscular fat have a higher market price, so how to increase the fat content in muscle is one of the important scientific and technological tasks in the field of animal nutrition. Commonly used livestock breeds for meat (meat pigs, beef cattle and meat sheep) are mainly lean meat types, which have the problems of low fat content in muscle and poor meat quality and flavor. Simply feeding high-fat feed will lead to fat deposition in the subc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/10A23K10/18A23K10/30A23K20/158A23K50/10A23K50/30A23K50/60
CPCA23K10/30A23K10/10A23K10/18A23K20/158A23K50/30A23K50/10A23K50/60A23V2400/321A23V2400/515
Inventor 高学军张明辉于萌萌祁昊李雪莹周成建金心
Owner YANGTZE UNIVERSITY