Glycosylated hemoglobin immunochromatographic test strip and preparation method thereof, and kit

A technology for glycated hemoglobin and immunochromatographic test strips, applied in the field of glycated hemoglobin immunochromatographic test strips and their preparation, can solve problems such as limited detection of HbA1c, and achieve the effects of short detection time, fast release speed, and quick operation.

Inactive Publication Date: 2020-03-10
海卫特(广州)医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Based on this, it is necessary to provide a glycosylated hemoglobin immunochromatographic test strip and its preparation method and kit for the limited detection of HbA1c in dogs and cats

Method used

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  • Glycosylated hemoglobin immunochromatographic test strip and preparation method thereof, and kit
  • Glycosylated hemoglobin immunochromatographic test strip and preparation method thereof, and kit
  • Glycosylated hemoglobin immunochromatographic test strip and preparation method thereof, and kit

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preparation example Construction

[0046] The present invention also provides a method for preparing any one of the above-mentioned glycosylated hemoglobin immunochromatographic test strips, comprising the following steps:

[0047] S1. Add the sample pad treatment solution to the sample pad and dry it.

[0048] The specific steps are: spray the sample pad treatment solution on the sample pad at a speed of 2-8 μL / cm, and dry it overnight.

[0049] In one embodiment, the sample pad treatment solution is a sample pad diluent containing 0.5-2 mg / mL mouse IgG, 0.5%-1% Evans blue and 0.1-2 mg / mL RBC, and the sample pad diluent is a sample pad diluent containing 0.5%- 3% Tween-20 and 2%-5% BSA (Bovine Serum Albumin) in 0.02M PB buffer (Phosphate Buffer).

[0050] S2. Use fluorescent latex particles to label the coupling complex of the first dog and cat HbA1c monoclonal antibody and the first complex, prepare a fluorescent latex particle-coupling complex labeling solution, and mix the labeling solution and the second ...

Embodiment 1

[0074] Preparation of labeling solution

[0075] 1), preparation of activated fluorescent microsphere solution:

[0076] Cleaning: Use a pipette gun to draw 1mL of fluorescent microsphere solution (containing 10mg of fluorescent microspheres) into a centrifuge tube, centrifuge at 14000rpm for 15min, discard the supernatant, add 1mL of 0.01M Tris buffer, and mix well by ultrasonic to obtain the washed Fluorescent microsphere solution.

[0077] Activation: Add 1 mL of 5 mg / mL N-hydroxysuccinimide and 5 mg / mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide to the above washed fluorescent microsphere solution The 0.01M Tris buffer solution of hydrochloride was mixed on a rotary mixer for 20 minutes to obtain an activated fluorescent microsphere solution.

[0078] Termination of activation: Centrifuge the above-mentioned activated fluorescent microspheres at 14000 rpm for 15 min, discard the supernatant, add 1 mL of 0.1M MES buffer containing 0.1% methanol, and ultrasonically mix t...

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Abstract

The invention relates to a glycosylated hemoglobin immunochromatographic test strip and a preparation method thereof and a kit. The test strip comprises a substrate, and a sampling pad, a coating filmand an absorption pad which are arranged on the substrate, wherein the sampling pad, the coating film and the absorption pad are sequentially connected from one end of the substrate to the other endof the substrate; a marking area, a detection area and a quality control area are sequentially arranged on the coating film; the marking area is coated with a coupling compound marked by fluorescent latex particles, and the coupling compound is formed by coupling a first dog and cat HbA1c monoclonal antibody and a first complex; the detection area is coated with a second dog and cat HbA1c monoclonal antibody; the quality control area is coated with a second complex, and the first complex and the second complex can be specifically combined; and the first dog and cat HbA1c monoclonal antibody can be specifically combined with hemoglobin, and the second dog and cat HbA1c monoclonal antibody can be specifically combined with glycosylated hemoglobin. The glycosylated hemoglobin immunochromatographic test strip provided by the invention achieves quantitative detection of glycosylated hemoglobin of dogs and cats, and has the characteristics of rapidness and simplicity.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to a glycosylated hemoglobin immunochromatography test strip, a preparation method and a kit thereof. Background technique [0002] Glycosylated hemoglobin (HbA1c) is a stable compound of glucose and the N-terminal valine residue of the beta chain of hemoglobin. In the body, hemoglobin contacts with glucose to undergo non-enzymatic glycosylation. In the initial stage, an unstable Schiff base (precursor of HbA1c) is formed, and then HbA1c is formed through Amadori rearrangement. [0003] The detection of HbA1c is the most representative means of diagnosing diabetes so far (every 1% change in HbA1c is equal to 2mmol / L change in blood sugar), which can not only reflect blood sugar control, treatment compliance and other indicators, but also play an important role in the prediction, diagnosis and prognosis of diabetes is also of great value. [0004] Traditional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72G01N33/577G01N33/558G01N33/543
CPCG01N33/54313G01N33/54326G01N33/558G01N33/577G01N33/723
Inventor 梁才弗黄晨珠杨晶邓艳珍岑赞询王伟
Owner 海卫特(广州)医疗科技有限公司
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