Primers for identifying types of rice GS5 gene and GLW7 gene and application of primers for identifying types of rice GS5 gene and GLW7 gene

A genotype and gene technology, applied in the field of primers to identify rice GS5 gene and GLW7 gene type, can solve the problems of high toxicity of gel components, cumbersome gel making process, and large fragment differences, so as to speed up the efficiency, process, and amplification Good effect, effect of reducing difficulty
CN110894542APending Publication Date: 2020-03-20YANGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YANGZHOU UNIV
Publication Date
2020-03-20

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Abstract

The invention discloses primers for identifying types of a GS5 gene and a GLW7 gene and application of the primers for identifying the types of the GS5 gene and the GLW7 gene, and belongs to the technical field of rice variety identification. According to the primers for identifying the types of the GS5 gene and the GLW7 gene and application of the primers for identifying the types of the GS5 geneand the GLW7 gene, by turning simply-repeated sequences of the rice GS5 gene and the rice GLW7 gene into digestion molecule markers, the types of the GS5 gene and the GLW7 gene are identified, and nucleotide sequences of the primers for identifying the types of the GS5 gene and the GLW7 gene are shown in SEQ ID NO.: 1-6 as shown in the description. By means of the molecule markers of the primersfor identifying the types of the GS5 gene and the GLW7 gene, the favorable variation types of the GS5 gene and the GLW7 gene can be accurately identified, the breeding screening efficiency can be improved, and a breeding screening process can be sped up; and the designed digestion molecule markers have good amplification effects, and selected enzymes are common enzymes which are low in price so that the identification cost can be greatly reduced, and the primers for identifying the types of the GS5 gene and the GLW7 gene are suitable for genotype analysis of a large number of varieties.
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Description

technical field

[0001] The invention belongs to the technical field of rice variety identification, and in particular relates to a primer for identifying rice GS5 gene and GLW7 gene type and application thereof. Background technique

[0002] Rice is an important food crop. With the development of my country's economy and the improvement of people's living standards, higher requirements are put forward for rice varieties. However, the current traditional variety improvement takes a long time, and requires planting a large number of populations and screening target traits with the aid of naked eye observation. With the improvement of rice genome information and continuous breakthroughs in functional gene research, the use of molecular marker-assisted selection for directional improvement of varieties has become a effective means. SSR marker is a relatively common molecular marker, which is characterized by a simple repetition of a nucleic acid base sequence. The marker can b...

Claims

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