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Hematopoietic stem cell cryopreservation solution and hematopoietic stem cell cryopreservation method

A technology of hematopoietic stem cells and cryopreservation methods, which is applied in the field of hematopoietic stem cell cryopreservation solution and hematopoietic stem cell cryopreservation, which can solve the problems of stem cell preservation effects and preservation time differences, large differences in the use of protective agents, and difficulty in ensuring the safety of stem cells. Achieve the effects of reducing cell ice crystal damage, maintaining physiological functions and biological characteristics, and prolonging storage time

Inactive Publication Date: 2020-03-24
贵州汉氏联合生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the protective agents used for low-temperature preservation of stem cells at -80°C include dimethyl sulfoxide, CP-1, hydroxyethyl starch, RPMI1640 medium, human serum albumin, etc. The formulas and methods of use of different protective agents are quite different. Large, there are differences in the preservation effect and storage time of stem cells. The survival rate of most preserved cells is about 80%. It is difficult to guarantee the safety of stem cells. Some cryopreservation methods also have the disadvantage of complicated operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, a hematopoietic stem cell cryopreservation solution and a hematopoietic stem cell cryopreservation method, the cryopreservation solution is composed of a cryoprotectant, a medium and human serum albumin, and the cryoprotectant is composed of the following components:

[0030] Dimethyl sulfoxide, hydroxyethyl starch, normal saline, polyvinylpyrrolidone;

[0031] The culture medium is composed of the following components: radish sugar, amino acid, fetal sheep serum;

[0032] The cryoprotectant comprises the following ingredients in parts by weight: dimethyl sulfoxide 25ml, hydroxyethyl starch 5g, physiological saline 15ml, polyvinylpyrrolidone 5g;

[0033] The culture medium includes the following components in parts by weight: 1.5 g of radish sugar, 25 g of amino acids, and 20 g of fetal sheep serum.

[0034] The cryopreservation method comprises the following steps:

[0035] Step 1: Weigh the cryoprotectant, culture medium and human serum albumin of each...

Embodiment 2

[0039] Embodiment 2, a hematopoietic stem cell cryopreservation solution and a hematopoietic stem cell cryopreservation method, the cryopreservation solution is composed of a cryoprotectant, a medium and human serum albumin, and the cryoprotectant is composed of the following components:

[0040] Dimethyl sulfoxide, hydroxyethyl starch, normal saline, polyvinylpyrrolidone;

[0041] The culture medium is composed of the following components: radish sugar, amino acid, fetal sheep serum and transferrin;

[0042] The cryoprotectant comprises the following ingredients in parts by weight: dimethyl sulfoxide 25ml, hydroxyethyl starch 5g, physiological saline 15ml, polyvinylpyrrolidone 5g;

[0043] The culture medium includes the following components in parts by weight: 1.5 g of radish sugar, 25 g of amino acids, 20 g of fetal sheep serum, and 5 g of transferrin.

[0044] The cryopreservation method comprises the following steps:

[0045] Step 1: Weigh the cryoprotectant, culture me...

Embodiment 3

[0049] Embodiment 3, a hematopoietic stem cell cryopreservation solution and a hematopoietic stem cell cryopreservation method, the cryopreservation solution is composed of a cryoprotectant, a culture medium and human serum albumin, and the cryoprotectant is composed of the following components:

[0050] Dimethyl sulfoxide, hydroxyethyl starch, normal saline, polyvinylpyrrolidone;

[0051] The culture medium is composed of the following components: radish sugar, amino acid, fetal sheep serum;

[0052] The cryoprotectant comprises the following ingredients in parts by weight: dimethyl sulfoxide 20ml, hydroxyethyl starch 7g, physiological saline 15ml, polyvinylpyrrolidone 5g;

[0053] The culture medium includes the following components in parts by weight: 0.8 g of radish sugar, 20 g of amino acids, and 25 g of fetal sheep serum.

[0054] The cryopreservation method comprises the following steps:

[0055]Step 1: Weigh the cryoprotectant, culture medium and human serum albumin ...

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PUM

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Abstract

The invention discloses a hematopoietic stem cell cryopreservation solution and a hematopoietic stem cell cryopreservation method. The cryopreservation solution is composed of a cryoprotectant, a culture medium and human serum albumin, and the cryoprotectant comprises 25 ml of dimethyl sulfoxide, 5 g of hydroxyethyl starch, 15 ml of normal saline and 5 g of polyvinylpyrrolidone; and the culture medium comprises 1.5 g of carrot sugar, 25 g of amino acids and 20 g of fetal sheep serum. The cryopreservation method has the advantages of simplicity in operation, low technical requirements, timely cryopreservation, solving of the problem that the traditional hematopoietic stem cells can be cryopreserved after several days of culture and observation, and provision of reliable guarantee for storage, application and transplantation of the hematopoietic stem cells.

Description

technical field [0001] The invention belongs to the technical field of cell cryopreservation, in particular to a hematopoietic stem cell cryopreservation solution and a hematopoietic stem cell cryopreservation method. Background technique [0002] Hematopoietic stem cells are adult stem cells in the blood system. They are a heterogeneous group with the ability of long-term self-renewal and the potential to differentiate into various mature blood cells. It is a type of adult stem cell with the longest and most in-depth research history, and it has important guiding significance for the study of various stem cells, including tumor stem cells. Mature cells in the blood system have a very short lifespan. Therefore, in a person's life, hematopoietic stem cells need to replenish various mature cell components of the blood system in a timely manner according to the physiological needs of the body. At the same time, under stress conditions such as injury and inflammation, hematopoi...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 戴志华戴媛潘洪旭
Owner 贵州汉氏联合生物技术有限公司
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