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Yeast hydrolysate with high free nucleotide and preparation method and application of yeast hydrolysate

A yeast hydrolyzate, nucleotide technology, applied in the application, animal feed, animal feed and other directions, can solve the problem of low free nucleotide content, increase the breeding efficiency, the preparation method is simple, and ease the use of feed nucleotides. The effect of scarcity

Pending Publication Date: 2020-03-31
ANGELYEAST CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a yeast hydrolyzate with high free nucleotides and its preparation method to solve the problem of low content of free nucleotides in yeast hydrolyzates for feeding in the market and meet the nutritional needs of young animals for nucleotides , to maximize production efficiency

Method used

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  • Yeast hydrolysate with high free nucleotide and preparation method and application of yeast hydrolysate
  • Yeast hydrolysate with high free nucleotide and preparation method and application of yeast hydrolysate
  • Yeast hydrolysate with high free nucleotide and preparation method and application of yeast hydrolysate

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preparation example Construction

[0060] The invention provides a kind of preparation method of yeast hydrolyzate, it comprises the following steps:

[0061] (1) Fermentation culture: fermenting and culturing the Saccharomyces cerevisiae strain to obtain the first yeast milk;

[0062] (2) Autolysis and enzymolysis: heat-shock treatment is performed on the yeast milk that accounts for 60-80% by weight of step (1), preferably 65-70% by weight of the first yeast milk, to perform autolysis, and then add papain Enzymolysis to obtain the second yeast milk;

[0063] (3) Nucleotidation: adding yeast RNA and nuclease to the second yeast milk obtained in step (2) for enzymolysis to obtain the third yeast milk, preferably adding yeast RNA first, and then adding nuclease for enzymolysis;

[0064] (4) Nucleotide enhancement: adding nucleotide disodium salt to the third yeast milk obtained in step (3) to obtain high free nucleotide yeast hydrolyzate.

[0065] In a preferred embodiment of the present invention, wherein, in...

Embodiment 1

[0090] Example 1 Preparation of Yeast Hydrolyzate

[0091] (1) Yeast fermentation culture

[0092] The culture solution contains carbon source, nitrogen source and phosphorus source, wherein the carbon source is 7500g of 35% molasses, the nitrogen source is 450g of ammonium sulfate, and the phosphorus source is 420g of ammonium dihydrogen phosphate. Sterilize the culture solution at 121°C for 10 minutes, feed it into the tank, inoculate yeast to carry out fermentation culture, the fermentation temperature is 29°C, the fermentation time is 14 hours, the fermentation pH is 4.5, and the pure culture liquid is fermented to obtain the first yeast milk (wherein nitrogen The mass is 144.9g, and the amount of phosphorus is 113.4g).

[0093] (2) Yeast autolysis and enzymolysis

[0094] Heat-shock the yeast milk that accounts for 60% by weight of the first yeast milk in step (1) at a temperature of 85° C. for 55 seconds, control the temperature at 45° C., add citric acid accounting fo...

Embodiment 2

[0113] (1) Yeast fermentation culture

[0114] The culture solution contains carbon source, nitrogen source and phosphorus source, wherein the carbon source is 8000g of 35% molasses, the nitrogen source is 400g of ammonium sulfate, and the phosphorus source is 450g of ammonium dihydrogen phosphate. Sterilize the culture solution at 121°C for 10 minutes, feed it into the tank, inoculate yeast to carry out fermentation culture, the fermentation temperature is 30°C, the fermentation time is 15 hours, and the fermentation pH is 5.0 to obtain the first fermented yeast milk of pure culture liquid (wherein nitrogen The mass is 138g, and the amount of phosphorus is 121.5g).

[0115] (2) Yeast autolysis and enzymolysis

[0116] Heat-shock the yeast milk that accounts for 65% by weight of the first yeast milk in step (1) at a temperature of 88°C for 60s, then control the temperature at 50°C, add citric acid accounting for 4‰ of the added weight of the dry matter of the yeast milk, and ...

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Abstract

The invention relates to the field of biological fermentation and feed, in particular to a yeast hydrolysate with high free nucleotide and a preparation method and application thereof. The yeast hydrolysate with high free nucleotide comprises protein, free nucleotide, amino acid and small peptide, wherein the content of the protein is 60-75% and the content of the free nucleotide is 35% or above based on the dry matter mass of the yeast hydrolysate, and the yeast hydrolysate is obtained through yeast milk enzymolysis, nucleotide acidification and nucleotide strengthening. The invention furtherprovides the preparation method of the yeast hydrolysate. The content of the free nucleotide in the prepared yeast hydrolysate is 35% or above, the preparation method is simple, and the obtained yeast hydrolysate is a natural product and is green and safe. The yeast hydrolysate with high free nucleotide can relieve the scarcity of a feeding nucleotide source, the breeding benefit is increased, and application prospects are broad.

Description

technical field [0001] The invention relates to the fields of biological fermentation and feed, in particular to a yeast hydrolyzate with high free nucleotides and its preparation method and application. Background technique [0002] Nucleotides are of great significance for animals to maintain, improve and repair gastrointestinal function. Supplementing exogenous nucleotides can accelerate the differentiation, growth and repair of intestinal cells, promote the maturation of the small intestine, and promote the growth of bifidobacteria and lactobacilli in the intestines. grow. Hoffmann (Hoffmann. The use of nucleotides in animal feed. Feed Mix, 2008 (15): 14-16.) research showed that after adding nucleotides to pig diets, the ratio between E. A change in direction in favor of beneficial microorganisms. Sauer et al. (Sauer, Bauer, Vahjen et al., Nucleotides modify growth of selected intestinal bacteria in vitro. Livestock Science, 2010, 133 (1): 161-163) mentioned that in v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/16A23K20/153A23K20/163A23K20/26A23K20/20
CPCA23K10/16A23K20/153A23K20/163A23K20/26A23K20/20
Inventor 陈中平俞学锋李知洪胡骏鹏廖汉江戴晋军王建林徐杰蔡大亮
Owner ANGELYEAST CO LTD
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