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Selective culture medium for separating Fusarium graminearum species complex from air

A technology of Fusarium graminearum and culture medium, applied in the field of microorganisms, can solve the problems of non-target fungi and bacteria contamination, difficult to distinguish, etc., and achieves the effects of high accuracy, practicality, fast cost, and easy preparation

Inactive Publication Date: 2020-03-31
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the invention provides a kind of selective medium for isolating Fusarium graminearum composite species from the air, which contains toxin, hymexazol, fosfo-aluminum, pentachlorin Benzene and rifampicin, using this medium can quickly isolate the complex species of Fusarium graminearum from the air, and can solve the problems of difficulty in distinguishing from other Fusarium and serious contamination of various non-target fungi and bacteria during the isolation process

Method used

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  • Selective culture medium for separating Fusarium graminearum species complex from air
  • Selective culture medium for separating Fusarium graminearum species complex from air
  • Selective culture medium for separating Fusarium graminearum species complex from air

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1, four kinds of fungal inhibitors and three kinds of antibiotics are to the inhibition of Fusarium graminearum one, four kinds of fungal inhibitors are to the inhibition of Fusarium graminearum complex species

[0028] Dissolve phosphonium aluminum with methanol and make 10 4mg / L, dissolve pentachloronitrobenzene in chloroform to make 2000mg / L, dissolve hymexazol and toxin in sterile water, and prepare 2000mg / L and 2.5×10 3 Two kinds of medicament mother liquors of mg / L; Among them, the drug-containing PDA plate with a series concentration of 50mg / L and 100mg / L is prepared with fosfoam; the series concentration of pentachloronitrobenzene is 10mg / L and 50mg / L Drug-containing PDA flat plate; prepare hymexazol with a series concentration of 5mg / L, 20mg / L, 50mg / L and 100mg / L drug-containing PDA flat plate; prepare the mother liquor of toxin preparation with a series concentration of 40mg / L, 60mg / L and 100mg / L drug-containing PDA plates, with the plates added wi...

Embodiment 2

[0037] Embodiment 2, three kinds of antibiotics are to the inhibitory effect of bacteria in the environment

[0038] Test sample: The atmosphere from the wheat planting field of the Institute of Plant Protection, Soil and Fertilizer, Hubei Academy of Agricultural Sciences, Hongshan District, Wuhan City in 2019.

[0039] Test method: This test is based on the results of Example 1 to detect whether the selected three concentrations of antibiotics can effectively inhibit the bacteria in the farmland atmosphere. For the detection of bacteria in the environment, it is mainly determined that the primary infection source of wheat head blight is mainly from the ascospores released from the diseased residue in the field and the diseased soil. In the field where the head blight is seriously ill, a flat plate containing 50 and 100 mg / L of rifampicin, penicillin and streptomycin sulfate is placed respectively, and the flat plate without medicine is used as a contrast, and each treatment i...

Embodiment 3

[0042] Embodiment 3, be used for isolating the PDA selective culture medium of Fusarium graminearum complex species

[0043] 1. Separation method

[0044] In 2019, the Fusarium graminearum compound species in the air was isolated from the wheat head blight resistance identification field of the Institute of Plant Protection, Soil and Fertilizer Research, Hongshan District, Wuhan City, Hubei Province. Separation is carried out as follows: select different fields in the same area for separation.

[0045] Separation step: place the selective culture medium of the present invention at 10 cm above the wheat canopy, place it in the air for about 30-60 minutes, bring it back to the laboratory and cultivate it in the dark at 25°C, and grow mycelia in 1-2 days Get the bacterium cake at the edge of the bacterium colony, place it on the common PDA medium and cultivate it, until the bacterium colony grows, obtain the Fusarium graminearum composite species ( figure 2 ). The obtained co...

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Abstract

The invention discloses a selective culture medium for separating a Fusarium graminearum species complex from the air. According to the culture medium, toxoflavin, hymexazol, aliette, quintozene and Rifampicin are added to a PDA culture medium. It is proved through experiments that when the selective culture medium is applied to separation of Fusarium graminearum species complex in the air, deep-wine characteristic colonies of Fusarium graminearum appear, and the problems can be solved that Fusarium graminearum cannot be distinguished easily from other Fusarium, and serious contamination is caused by multiple undesired fungi and bacteria during separation; and the selective culture medium has the characteristics of feasibility, high accuracy, practicality, quickness and simple preparation.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a selective culture medium for simple and rapid separation of Fusarium graminearum complex species in the air. Background technique [0002] Fusarium Head Blight (FHB) is a devastating disease of wheat and barley worldwide. This disease not only causes severe yield loss, but also leads to the contamination of grains used in the food and feed industry with mycotoxins, causing huge indirect losses. In China, Fusarium graminearum (Fusarium graminearum) and Asian Fusarium (Fusarium asiaticum) are the main pathogens causing wheat head blight, accounting for more than 90% of the isolated proportion, called Fusarium graminearum Species Complex (Fusarium graminearum Species Complex) , FGSC); the main pathogen in America and Europe is Fusarium graminearum (F. graminearum). Fusarium graminearum passes through asexual and sexual stages (Gibberrllazeae) in its life cycle. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12R1/77
CPCC12N1/02C12N1/14
Inventor 杨立军龚双军史文琦向礼波曾凡松袁斌薛敏峰陈婷婷刘美玲喻大昭
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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