Latex immunoturbidimetric detection kit for quantitatively determining cat serum amyloid protein A

Abstract

The invention relates to a latex immunoturbidimetry detection kit for quantitatively determining cat serum amyloid protein A. The latex immunoturbidimetry detection kit comprises reagent beads formedby freeze-drying polystyrene latex particles sensitized by a cat serum amyloid protein A antibody, a reagent disc for detection, water cup water for diluting a sample, a cat serum amyloid protein A calibration product and a quality control product. The product can accurately and quantitatively detect the content of the cat serum amyloid protein A, can monitor the early inflammation of infectious diseases according to the content of the cat serum amyloid protein A, is beneficial to diagnosing inflammation, evaluating the activity of the cat serum amyloid protein A and monitoring the activity and treatment of the cat serum amyloid protein A, and has very high clinical value. The kit has the advantages of high specificity, high sensitivity, high precision, high accuracy, simplicity, convenience, quickness and the like.

Description

technical field [0001] The invention relates to the technical field of immune analysis, in particular to a latex immunoturbidimetric detection kit for quantitatively measuring feline serum amyloid A. Background technique [0002] Feline serum amyloid A (SAA) is a precursor of amyloid deposition, which was discovered during the study of amyloidosis, and subsequently acts as an acute phase response protein, which belongs to the heterogeneity of the apolipoprotein family protein-like. Feline serum amyloid A is synthesized in the liver by activated macrophages and fibroblasts and can be elevated in the acute phase response to stimulation with interleukin-1, interleukin-6, and tumor necrosis factor to 100 -1000 times the initial concentration. During inflammation, feline serum amyloid A binds to plasma high-density lipoprotein (HDL) and regulates HDL metabolism. In addition, feline serum amyloid A degradation products can be deposited in different organs as amyloid A (AA) fibr...

AI Technical Summary

Problems solved by technology

[0006] However, when the serum amyloid A antibody is coated on the latex, physical adsorption and chemical coupling methods can generally be used. The stability of the sensitized latex particles obtained by the physical adsorption method is worse than that of the chemical coupling method, and the antibody is easily released from the latex particles. and the latex obtained by physical adsorption may be interfered by rheumatoid factor (RF) and heterophilic antibodies. IgM and IgG type RF can directly bind to the Fc segment of the antibody coated on the latex, thereby Cause false positive or false negative test results to rise

Most of the chemical coupling methods currently used are random coupling methods, because randomly coupled antibodies are randomly coupled to different parts of the antibody, which will lead to the loss of binding force of the antibody; therefore, complete random coupling will lose most of the binding force of the antibody and increase Antibody dosage and production cost

At the same time, if the sensitized latex particles obtained by the above method are used in the detection kit, it will cause the defects of poor specificity, low accuracy, weak anti-interference ability or high production cost.

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